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Histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system

Meniscus plays fundamental roles in the knee mechanisms and functions but injuries happen in meniscus have poor healing ability that requires great interventions. Tissue engineered scaffolds serve as one of the interventions to regenerate the required tissue to treat early degenerative joint disease...

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Main Authors: Mohd. Yusof, Fatihah, Sha'ban, Munirah, Azhim, Azran
Format: Conference or Workshop Item
Language:English
English
English
Published: Institute of Electrical and Electronics Engineers Inc. 2019
Subjects:
R Medicine (General)
Online Access:http://irep.iium.edu.my/72620/1/72620_Histological%20and%20biochemical%20evaluations.pdf
http://irep.iium.edu.my/72620/2/72620_Histological%20and%20biochemical%20evaluations_SCOPUS.pdf
http://irep.iium.edu.my/72620/3/72620_Histological%20and%20biochemical%20evaluations_WOS.pdf
http://irep.iium.edu.my/72620/
https://ieeexplore.ieee.org/document/8626689
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spelling my.iium.irep.726202019-11-24T11:17:43Z http://irep.iium.edu.my/72620/ Histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system Mohd. Yusof, Fatihah Sha'ban, Munirah Azhim, Azran R Medicine (General) Meniscus plays fundamental roles in the knee mechanisms and functions but injuries happen in meniscus have poor healing ability that requires great interventions. Tissue engineered scaffolds serve as one of the interventions to regenerate the required tissue to treat early degenerative joint disease. The purpose of this research is to examine the effectiveness of sonication treatment system in complete cellular components removal with preserved extracellular matrix (ECM) compositions in meniscus tissues through histological and biochemical evaluations. Meniscus tissues were decellularized using sonication treatment system for 10 hours treatment time and continued with extensive washing process. Histological evaluations were based on van Gieson and Picrosirius red staining that portrayed complete cellular components removal and preserved collagen networks distribution within sonicated scaffolds respectively. Biochemical evaluations showed markedly reduction in the residual DNA content for sonicated scaffolds while maintain in collagen content. Lastly, agarose gel electrophoresis showed no visible DNA bands for sonicated scaffolds. Therefore, the results concluded that the prepared bioscaffolds by sonication treatment system managed to reduce the immunogenicity of scaffolds by removing most of the cellular components and successfully retained the properties of the extracellular matrix. Thus, it is a suitable decellularization method to be used in tissue engineering applications. Institute of Electrical and Electronics Engineers Inc. 2019-01-24 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/72620/1/72620_Histological%20and%20biochemical%20evaluations.pdf application/pdf en http://irep.iium.edu.my/72620/2/72620_Histological%20and%20biochemical%20evaluations_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/72620/3/72620_Histological%20and%20biochemical%20evaluations_WOS.pdf Mohd. Yusof, Fatihah and Sha'ban, Munirah and Azhim, Azran (2019) Histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system. In: 2018 IEEE EMBS Conference on Biomedical Engineering and Sciences )IECBES 2018), 3rd-6th December 2018, Kuching, Sarawak. https://ieeexplore.ieee.org/document/8626689 10.1109/IECBES.2018.8626689
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
English
topic R Medicine (General)
spellingShingle R Medicine (General)
Mohd. Yusof, Fatihah
Sha'ban, Munirah
Azhim, Azran
Histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system
description Meniscus plays fundamental roles in the knee mechanisms and functions but injuries happen in meniscus have poor healing ability that requires great interventions. Tissue engineered scaffolds serve as one of the interventions to regenerate the required tissue to treat early degenerative joint disease. The purpose of this research is to examine the effectiveness of sonication treatment system in complete cellular components removal with preserved extracellular matrix (ECM) compositions in meniscus tissues through histological and biochemical evaluations. Meniscus tissues were decellularized using sonication treatment system for 10 hours treatment time and continued with extensive washing process. Histological evaluations were based on van Gieson and Picrosirius red staining that portrayed complete cellular components removal and preserved collagen networks distribution within sonicated scaffolds respectively. Biochemical evaluations showed markedly reduction in the residual DNA content for sonicated scaffolds while maintain in collagen content. Lastly, agarose gel electrophoresis showed no visible DNA bands for sonicated scaffolds. Therefore, the results concluded that the prepared bioscaffolds by sonication treatment system managed to reduce the immunogenicity of scaffolds by removing most of the cellular components and successfully retained the properties of the extracellular matrix. Thus, it is a suitable decellularization method to be used in tissue engineering applications.
format Conference or Workshop Item
author Mohd. Yusof, Fatihah
Sha'ban, Munirah
Azhim, Azran
author_facet Mohd. Yusof, Fatihah
Sha'ban, Munirah
Azhim, Azran
author_sort Mohd. Yusof, Fatihah
title Histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system
title_short Histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system
title_full Histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system
title_fullStr Histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system
title_full_unstemmed Histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system
title_sort histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system
publisher Institute of Electrical and Electronics Engineers Inc.
publishDate 2019
url http://irep.iium.edu.my/72620/1/72620_Histological%20and%20biochemical%20evaluations.pdf
http://irep.iium.edu.my/72620/2/72620_Histological%20and%20biochemical%20evaluations_SCOPUS.pdf
http://irep.iium.edu.my/72620/3/72620_Histological%20and%20biochemical%20evaluations_WOS.pdf
http://irep.iium.edu.my/72620/
https://ieeexplore.ieee.org/document/8626689
_version_ 1651865919986597888
score 13.211869

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