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Inappropriate expression of an NLP effector in colletotrichum orbiculare impairs infection on cucurbitaceae cultivars via plant recognition of the C-terminal region

The hemibiotrophic pathogen Colletotrichum orbiculare preferentially expresses a necrosis and ethylene-inducing peptide 1 (Nep1)-like protein named NLP1 during the switch to necrotrophy. Here, we report that the constitutive expression of NLP1 in C. orbiculare blocks pathogen infection in multiple C...

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Main Authors: Ahmad Azmi, Nur Sabrina, Ogawa, Suthitar Singkaravanit, Ikeda, Kyoko, Kitakura, Saeko, Inoue, Yoshihiro, Narusaka, Yoshihiro, Shirasu, Ken, Kaido, Masanori, Mise, Kazuyuki, Takano, Yoshitaka
Format: Article
Language:English
English
Published: American Phytopathological Society 2018
Subjects:
S Agriculture (General)
Online Access:http://irep.iium.edu.my/70722/1/70722_Inappropriate%20Expression%20of%20an%20NLP%20Effector.pdf
http://irep.iium.edu.my/70722/2/70722_Inappropriate%20Expression%20of%20an%20NLP%20Effector_SCOPUS.pdf
http://irep.iium.edu.my/70722/
https://apsjournals.apsnet.org/doi/pdf/10.1094/MPMI-04-17-0085-FI
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spelling my.iium.irep.707222019-03-03T02:12:34Z http://irep.iium.edu.my/70722/ Inappropriate expression of an NLP effector in colletotrichum orbiculare impairs infection on cucurbitaceae cultivars via plant recognition of the C-terminal region Ahmad Azmi, Nur Sabrina Ogawa, Suthitar Singkaravanit Ikeda, Kyoko Kitakura, Saeko Inoue, Yoshihiro Narusaka, Yoshihiro Shirasu, Ken Kaido, Masanori Mise, Kazuyuki Takano, Yoshitaka S Agriculture (General) The hemibiotrophic pathogen Colletotrichum orbiculare preferentially expresses a necrosis and ethylene-inducing peptide 1 (Nep1)-like protein named NLP1 during the switch to necrotrophy. Here, we report that the constitutive expression of NLP1 in C. orbiculare blocks pathogen infection in multiple Cucurbitaceae cultivars via their enhanced defense responses. NLP1 has a cytotoxic activity that induces cell death in Nicotiana benthamiana. However, C. orbiculare transgenic lines constitutively expressing a mutant NLP1 lacking the cytotoxic activity still failed to infect cucumber, indicating no clear relationship between cytotoxic activity and the NLP1-dependent enhanced defense. NLP1 also possesses the microbe-associated molecular pattern (MAMP) sequence called nlp24, recognized by Arabidopsis thaliana at its central region, similar to NLPs of other pathogens. Surprisingly, inappropriate expression of a mutant NLP1 lacking the MAMP signature is also effective for blocking pathogen infection, uncoupling the infection block from the corresponding MAMP. Notably, the deletion analyses of NLP1 suggested that the C-terminal region of NLP1 is critical to enhance defense in cucumber. The expression of mCherry fused with the C-terminal 32 amino acids of NLP1 was enough to trigger the defense of cucurbits, revealing that the C-terminal region of the NLP1 protein is recognized by cucurbits and, then, terminates C. orbiculare infection. American Phytopathological Society 2018-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/70722/1/70722_Inappropriate%20Expression%20of%20an%20NLP%20Effector.pdf application/pdf en http://irep.iium.edu.my/70722/2/70722_Inappropriate%20Expression%20of%20an%20NLP%20Effector_SCOPUS.pdf Ahmad Azmi, Nur Sabrina and Ogawa, Suthitar Singkaravanit and Ikeda, Kyoko and Kitakura, Saeko and Inoue, Yoshihiro and Narusaka, Yoshihiro and Shirasu, Ken and Kaido, Masanori and Mise, Kazuyuki and Takano, Yoshitaka (2018) Inappropriate expression of an NLP effector in colletotrichum orbiculare impairs infection on cucurbitaceae cultivars via plant recognition of the C-terminal region. Molecular Plant-Microbe Interactions, 31 (1). pp. 101-111. ISSN 0894-0282 https://apsjournals.apsnet.org/doi/pdf/10.1094/MPMI-04-17-0085-FI 10.1094/MPMI-04-17-0085-FI
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic S Agriculture (General)
spellingShingle S Agriculture (General)
Ahmad Azmi, Nur Sabrina
Ogawa, Suthitar Singkaravanit
Ikeda, Kyoko
Kitakura, Saeko
Inoue, Yoshihiro
Narusaka, Yoshihiro
Shirasu, Ken
Kaido, Masanori
Mise, Kazuyuki
Takano, Yoshitaka
Inappropriate expression of an NLP effector in colletotrichum orbiculare impairs infection on cucurbitaceae cultivars via plant recognition of the C-terminal region
description The hemibiotrophic pathogen Colletotrichum orbiculare preferentially expresses a necrosis and ethylene-inducing peptide 1 (Nep1)-like protein named NLP1 during the switch to necrotrophy. Here, we report that the constitutive expression of NLP1 in C. orbiculare blocks pathogen infection in multiple Cucurbitaceae cultivars via their enhanced defense responses. NLP1 has a cytotoxic activity that induces cell death in Nicotiana benthamiana. However, C. orbiculare transgenic lines constitutively expressing a mutant NLP1 lacking the cytotoxic activity still failed to infect cucumber, indicating no clear relationship between cytotoxic activity and the NLP1-dependent enhanced defense. NLP1 also possesses the microbe-associated molecular pattern (MAMP) sequence called nlp24, recognized by Arabidopsis thaliana at its central region, similar to NLPs of other pathogens. Surprisingly, inappropriate expression of a mutant NLP1 lacking the MAMP signature is also effective for blocking pathogen infection, uncoupling the infection block from the corresponding MAMP. Notably, the deletion analyses of NLP1 suggested that the C-terminal region of NLP1 is critical to enhance defense in cucumber. The expression of mCherry fused with the C-terminal 32 amino acids of NLP1 was enough to trigger the defense of cucurbits, revealing that the C-terminal region of the NLP1 protein is recognized by cucurbits and, then, terminates C. orbiculare infection.
format Article
author Ahmad Azmi, Nur Sabrina
Ogawa, Suthitar Singkaravanit
Ikeda, Kyoko
Kitakura, Saeko
Inoue, Yoshihiro
Narusaka, Yoshihiro
Shirasu, Ken
Kaido, Masanori
Mise, Kazuyuki
Takano, Yoshitaka
author_facet Ahmad Azmi, Nur Sabrina
Ogawa, Suthitar Singkaravanit
Ikeda, Kyoko
Kitakura, Saeko
Inoue, Yoshihiro
Narusaka, Yoshihiro
Shirasu, Ken
Kaido, Masanori
Mise, Kazuyuki
Takano, Yoshitaka
author_sort Ahmad Azmi, Nur Sabrina
title Inappropriate expression of an NLP effector in colletotrichum orbiculare impairs infection on cucurbitaceae cultivars via plant recognition of the C-terminal region
title_short Inappropriate expression of an NLP effector in colletotrichum orbiculare impairs infection on cucurbitaceae cultivars via plant recognition of the C-terminal region
title_full Inappropriate expression of an NLP effector in colletotrichum orbiculare impairs infection on cucurbitaceae cultivars via plant recognition of the C-terminal region
title_fullStr Inappropriate expression of an NLP effector in colletotrichum orbiculare impairs infection on cucurbitaceae cultivars via plant recognition of the C-terminal region
title_full_unstemmed Inappropriate expression of an NLP effector in colletotrichum orbiculare impairs infection on cucurbitaceae cultivars via plant recognition of the C-terminal region
title_sort inappropriate expression of an nlp effector in colletotrichum orbiculare impairs infection on cucurbitaceae cultivars via plant recognition of the c-terminal region
publisher American Phytopathological Society
publishDate 2018
url http://irep.iium.edu.my/70722/1/70722_Inappropriate%20Expression%20of%20an%20NLP%20Effector.pdf
http://irep.iium.edu.my/70722/2/70722_Inappropriate%20Expression%20of%20an%20NLP%20Effector_SCOPUS.pdf
http://irep.iium.edu.my/70722/
https://apsjournals.apsnet.org/doi/pdf/10.1094/MPMI-04-17-0085-FI
_version_ 1643619263904219136
score 13.222552

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