Site-directed mutagenesis on plasmid using polymerase chain reaction
Site-directed mutagenesis (SDM) is a very useful technique to study changes in protein function that may occur as a result of the DNA manipulation. A detail procedure to employ SDM on whole plasmid using polymerase chain reaction (PCR) with successful application of the method on a pET28a plasmid ha...
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| Main Authors: | , , |
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| Format: | Book Chapter |
| Language: | English English |
| Published: |
Springer Nature
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/70243/1/70243_Site-directed%20mutagenesis%20on%20plasmid.pdf http://irep.iium.edu.my/70243/7/70243_Site-directed%20mutagenesis%20on%20plasmid_Scopus.pdf http://irep.iium.edu.my/70243/ https://www.springer.com/us/book/9789811322563 https://doi.org/10.1007/978-981-13-2257-0 |
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http://irep.iium.edu.my/70243/1/70243_Site-directed%20mutagenesis%20on%20plasmid.pdfhttp://irep.iium.edu.my/70243/7/70243_Site-directed%20mutagenesis%20on%20plasmid_Scopus.pdf
http://irep.iium.edu.my/70243/
https://www.springer.com/us/book/9789811322563
https://doi.org/10.1007/978-981-13-2257-0