Staff View: Spermatogonial stem cells protein marker identification from in vitro differentiation of nonobstructive azoospermia (NOA) testes biopsies cells: An approach in line with Maqasid Shariah to maintain the heredity.

Spermatogonial stem cells protein marker identification from in vitro differentiation of nonobstructive azoospermia (NOA) testes biopsies cells: An approach in line with Maqasid Shariah to maintain the heredity.

Azoospermia is present in 15% of infertile cases and it is a major concern due to inability to produce sperm. Most of IVF (in-vitro fertilization) clinics abroad has been using sperm donation via sperm bank facilities as a solution for infertile couple to have their own offspring. In Islam, it is...

Full description

Saved in:

Bibliographic Details
Main Authors:	Abdul Wahab, Azantee Yazmie, Ramli, Roszaman, Ahmad Arifin, Syamsul, Mat Yusof, Afzan, Abdul Razak, Redzuan Nul Hakim, Abdullah, Afif Raihan, Muhammad, Husin, Md Isa, Muhammad Lokman
Format:	Article
Language:	English English
Published:	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2018
Subjects:	RG Gynecology and obstetrics RT Nursing
Online Access:	http://irep.iium.edu.my/69832/1/69832_Spermatogonial%20stem%20cells%20protein%20marker%20identification%20from%20in%20vitro_article.pdf http://irep.iium.edu.my/69832/7/69832%20SCOPUS.pdf http://irep.iium.edu.my/69832/ http://iiumedic.net/imjm/v1/download/volume_17_special_issue_2/Pages-from-2WCII-SI2-149.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.iium.irep.69832
record_format	dspace
spelling	my.iium.irep.698322020-08-07T00:18:30Z http://irep.iium.edu.my/69832/ Spermatogonial stem cells protein marker identification from in vitro differentiation of nonobstructive azoospermia (NOA) testes biopsies cells: An approach in line with Maqasid Shariah to maintain the heredity. Abdul Wahab, Azantee Yazmie Ramli, Roszaman Ahmad Arifin, Syamsul Mat Yusof, Afzan Abdul Razak, Redzuan Nul Hakim Abdullah, Afif Raihan Muhammad, Husin Md Isa, Muhammad Lokman RG Gynecology and obstetrics RT Nursing Azoospermia is present in 15% of infertile cases and it is a major concern due to inability to produce sperm. Most of IVF (in-vitro fertilization) clinics abroad has been using sperm donation via sperm bank facilities as a solution for infertile couple to have their own offspring. In Islam, it is forbidden to use sample from male other than their spouse. It is according to maqasid syari’ah to ensure the heredity of the human being. Based on the latest technology, one approach of stem cell differentiation process had been established to produce mature cells from primitive or immature cells (stem cells). This technology is in line with the concept of maqasid syari’ah since we are using the cells from one person. We try to adopt this technology to study the potential of testicular cells from non-obstructive azoospermic (NOA) patient to undergo in vitro spermatogenesis. Samples were cultured in modified human embryonic stem cells (HESC) media with specific growth factors; basic fibroblast growth factor (bFGF) and leukemia inhibitory factor (LIF). Protein expressions were analyzed by immunofluorescent staining on day 49 and 90 of culture. Results show spermatogonial stem cell-like cells (SSC-like cells) colonies formed after 14 to 21 days, the cells were expanding successfully and were stable for 49 days duration. Then SSCs differentiated into later stage of spermatogenesis on day 90. Four specifics SSCs protein markers were identified on day 49; ITGA1, ITGB1, CD9 and GFRA1 whereas SCP3 and TP1 proteins were expressed on day 90. This in vitro spermatogenesis suggests a possible approach for future for Muslim NOA patients in order to have their own children. Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2018-10-18 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69832/1/69832_Spermatogonial%20stem%20cells%20protein%20marker%20identification%20from%20in%20vitro_article.pdf application/pdf en http://irep.iium.edu.my/69832/7/69832%20SCOPUS.pdf Abdul Wahab, Azantee Yazmie and Ramli, Roszaman and Ahmad Arifin, Syamsul and Mat Yusof, Afzan and Abdul Razak, Redzuan Nul Hakim and Abdullah, Afif Raihan and Muhammad, Husin and Md Isa, Muhammad Lokman (2018) Spermatogonial stem cells protein marker identification from in vitro differentiation of nonobstructive azoospermia (NOA) testes biopsies cells: An approach in line with Maqasid Shariah to maintain the heredity. The International Medical Journal Malaysia, 17 (Special Issue 2). pp. 149-156. E-ISSN 1823-4631 http://iiumedic.net/imjm/v1/download/volume_17_special_issue_2/Pages-from-2WCII-SI2-149.pdf
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English English
topic	RG Gynecology and obstetrics RT Nursing
spellingShingle	RG Gynecology and obstetrics RT Nursing Abdul Wahab, Azantee Yazmie Ramli, Roszaman Ahmad Arifin, Syamsul Mat Yusof, Afzan Abdul Razak, Redzuan Nul Hakim Abdullah, Afif Raihan Muhammad, Husin Md Isa, Muhammad Lokman Spermatogonial stem cells protein marker identification from in vitro differentiation of nonobstructive azoospermia (NOA) testes biopsies cells: An approach in line with Maqasid Shariah to maintain the heredity.
description	Azoospermia is present in 15% of infertile cases and it is a major concern due to inability to produce sperm. Most of IVF (in-vitro fertilization) clinics abroad has been using sperm donation via sperm bank facilities as a solution for infertile couple to have their own offspring. In Islam, it is forbidden to use sample from male other than their spouse. It is according to maqasid syari’ah to ensure the heredity of the human being. Based on the latest technology, one approach of stem cell differentiation process had been established to produce mature cells from primitive or immature cells (stem cells). This technology is in line with the concept of maqasid syari’ah since we are using the cells from one person. We try to adopt this technology to study the potential of testicular cells from non-obstructive azoospermic (NOA) patient to undergo in vitro spermatogenesis. Samples were cultured in modified human embryonic stem cells (HESC) media with specific growth factors; basic fibroblast growth factor (bFGF) and leukemia inhibitory factor (LIF). Protein expressions were analyzed by immunofluorescent staining on day 49 and 90 of culture. Results show spermatogonial stem cell-like cells (SSC-like cells) colonies formed after 14 to 21 days, the cells were expanding successfully and were stable for 49 days duration. Then SSCs differentiated into later stage of spermatogenesis on day 90. Four specifics SSCs protein markers were identified on day 49; ITGA1, ITGB1, CD9 and GFRA1 whereas SCP3 and TP1 proteins were expressed on day 90. This in vitro spermatogenesis suggests a possible approach for future for Muslim NOA patients in order to have their own children.
format	Article
author	Abdul Wahab, Azantee Yazmie Ramli, Roszaman Ahmad Arifin, Syamsul Mat Yusof, Afzan Abdul Razak, Redzuan Nul Hakim Abdullah, Afif Raihan Muhammad, Husin Md Isa, Muhammad Lokman
author_facet	Abdul Wahab, Azantee Yazmie Ramli, Roszaman Ahmad Arifin, Syamsul Mat Yusof, Afzan Abdul Razak, Redzuan Nul Hakim Abdullah, Afif Raihan Muhammad, Husin Md Isa, Muhammad Lokman
author_sort	Abdul Wahab, Azantee Yazmie
title	Spermatogonial stem cells protein marker identification from in vitro differentiation of nonobstructive azoospermia (NOA) testes biopsies cells: An approach in line with Maqasid Shariah to maintain the heredity.
title_short	Spermatogonial stem cells protein marker identification from in vitro differentiation of nonobstructive azoospermia (NOA) testes biopsies cells: An approach in line with Maqasid Shariah to maintain the heredity.
title_full	Spermatogonial stem cells protein marker identification from in vitro differentiation of nonobstructive azoospermia (NOA) testes biopsies cells: An approach in line with Maqasid Shariah to maintain the heredity.
title_fullStr	Spermatogonial stem cells protein marker identification from in vitro differentiation of nonobstructive azoospermia (NOA) testes biopsies cells: An approach in line with Maqasid Shariah to maintain the heredity.
title_full_unstemmed	Spermatogonial stem cells protein marker identification from in vitro differentiation of nonobstructive azoospermia (NOA) testes biopsies cells: An approach in line with Maqasid Shariah to maintain the heredity.
title_sort	spermatogonial stem cells protein marker identification from in vitro differentiation of nonobstructive azoospermia (noa) testes biopsies cells: an approach in line with maqasid shariah to maintain the heredity.
publisher	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia
publishDate	2018
url	http://irep.iium.edu.my/69832/1/69832_Spermatogonial%20stem%20cells%20protein%20marker%20identification%20from%20in%20vitro_article.pdf http://irep.iium.edu.my/69832/7/69832%20SCOPUS.pdf http://irep.iium.edu.my/69832/ http://iiumedic.net/imjm/v1/download/volume_17_special_issue_2/Pages-from-2WCII-SI2-149.pdf
_version_	1675327135535857664
score	13.211869

Spermatogonial stem cells protein marker identification from in vitro differentiation of nonobstructive azoospermia (NOA) testes biopsies cells: An approach in line with Maqasid Shariah to maintain the heredity.

Similar Items