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Identification of Beta Human Chorionic Gonadotrophin (bHCG) expression in frozen cleavage embryo of In Vitro Fertilization (IVF) patients: An essential approach for better outcome towards human being inheritance in line with Maqasid Syari’ah concept

Expression of beta-human chorionic gonadotropin (bHCG) mRNA in early cleavage cell stages is important in predicting embryo viability at blastocyst stage of development. Embryo viability is crucial for in vitro fertilization (IVF) treatment to be successful in terms of increment of implantation an...

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Main Authors: Md Isa, Muhammad Lokman, Mat Yusof, Afzan, Ramli, Roszaman, Ahmad Arifin, Syamsul, Emalina, Fatin, Abdul Wahab, Azantee Yazmie
Format: Article
Language:English
Published: The International Medical Journal Malaysia 2018
Subjects:
RG Gynecology and obstetrics
RT Nursing
Online Access:http://irep.iium.edu.my/69829/1/Dr%20Roszaman-%20IMJM%20-NOA.pdf
http://irep.iium.edu.my/69829/
http://iiumedic.net/imjm/v1/download/volume_17_special_issue_2/Pages-from-2WCII-SI2-165.pdf
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spelling my.iium.irep.698292019-01-24T12:13:08Z http://irep.iium.edu.my/69829/ Identification of Beta Human Chorionic Gonadotrophin (bHCG) expression in frozen cleavage embryo of In Vitro Fertilization (IVF) patients: An essential approach for better outcome towards human being inheritance in line with Maqasid Syari’ah concept Md Isa, Muhammad Lokman Mat Yusof, Afzan Ramli, Roszaman Ahmad Arifin, Syamsul Emalina, Fatin Abdul Wahab, Azantee Yazmie RG Gynecology and obstetrics RT Nursing Expression of beta-human chorionic gonadotropin (bHCG) mRNA in early cleavage cell stages is important in predicting embryo viability at blastocyst stage of development. Embryo viability is crucial for in vitro fertilization (IVF) treatment to be successful in terms of increment of implantation and pregnancy rate. In order to establish fertilization outside the human body, optimum condition mimicking the natural body environment like hormones and growth factors as well as impeccable timing must be established. Imam Muslim narrated from Hudhayfa ibn Asad that the Prophet Muhammad (SAW) said: “After the sperm-andovum drop (nut.fa) has been [in the uterus] forty-two days, Allah sends it an angel that gives it form and fashions its hearing, sight, skin, flesh, and skeleton”. Therefore the aim of this study is to find the significant association of bHCG expression with early cell stage cleavages and its relationship as predictive marker for potential embryo implantation. Our study focuses on leftover frozen embryos from eight patients consists of six pregnant patients and two non-pregnant patients. We assessed the human bHCG mRNA expression at different cell cleavage stages in these frozen embryos using reverse transcriptasepolymerase chain reaction (RT-PCR). Our results have shown three out of eight patients with five to ten cells of blastomeres were expressed with bHCG. This study indicated that bHCG was expressed on frozethawed late cleavage stage of embryos in IVF patients. The International Medical Journal Malaysia 2018-10-18 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69829/1/Dr%20Roszaman-%20IMJM%20-NOA.pdf Md Isa, Muhammad Lokman and Mat Yusof, Afzan and Ramli, Roszaman and Ahmad Arifin, Syamsul and Emalina, Fatin and Abdul Wahab, Azantee Yazmie (2018) Identification of Beta Human Chorionic Gonadotrophin (bHCG) expression in frozen cleavage embryo of In Vitro Fertilization (IVF) patients: An essential approach for better outcome towards human being inheritance in line with Maqasid Syari’ah concept. The International Medical Journal Malaysia, 17 (2). pp. 165-170. http://iiumedic.net/imjm/v1/download/volume_17_special_issue_2/Pages-from-2WCII-SI2-165.pdf
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic RG Gynecology and obstetrics
RT Nursing
spellingShingle RG Gynecology and obstetrics
RT Nursing
Md Isa, Muhammad Lokman
Mat Yusof, Afzan
Ramli, Roszaman
Ahmad Arifin, Syamsul
Emalina, Fatin
Abdul Wahab, Azantee Yazmie
Identification of Beta Human Chorionic Gonadotrophin (bHCG) expression in frozen cleavage embryo of In Vitro Fertilization (IVF) patients: An essential approach for better outcome towards human being inheritance in line with Maqasid Syari’ah concept
description Expression of beta-human chorionic gonadotropin (bHCG) mRNA in early cleavage cell stages is important in predicting embryo viability at blastocyst stage of development. Embryo viability is crucial for in vitro fertilization (IVF) treatment to be successful in terms of increment of implantation and pregnancy rate. In order to establish fertilization outside the human body, optimum condition mimicking the natural body environment like hormones and growth factors as well as impeccable timing must be established. Imam Muslim narrated from Hudhayfa ibn Asad that the Prophet Muhammad (SAW) said: “After the sperm-andovum drop (nut.fa) has been [in the uterus] forty-two days, Allah sends it an angel that gives it form and fashions its hearing, sight, skin, flesh, and skeleton”. Therefore the aim of this study is to find the significant association of bHCG expression with early cell stage cleavages and its relationship as predictive marker for potential embryo implantation. Our study focuses on leftover frozen embryos from eight patients consists of six pregnant patients and two non-pregnant patients. We assessed the human bHCG mRNA expression at different cell cleavage stages in these frozen embryos using reverse transcriptasepolymerase chain reaction (RT-PCR). Our results have shown three out of eight patients with five to ten cells of blastomeres were expressed with bHCG. This study indicated that bHCG was expressed on frozethawed late cleavage stage of embryos in IVF patients.
format Article
author Md Isa, Muhammad Lokman
Mat Yusof, Afzan
Ramli, Roszaman
Ahmad Arifin, Syamsul
Emalina, Fatin
Abdul Wahab, Azantee Yazmie
author_facet Md Isa, Muhammad Lokman
Mat Yusof, Afzan
Ramli, Roszaman
Ahmad Arifin, Syamsul
Emalina, Fatin
Abdul Wahab, Azantee Yazmie
author_sort Md Isa, Muhammad Lokman
title Identification of Beta Human Chorionic Gonadotrophin (bHCG) expression in frozen cleavage embryo of In Vitro Fertilization (IVF) patients: An essential approach for better outcome towards human being inheritance in line with Maqasid Syari’ah concept
title_short Identification of Beta Human Chorionic Gonadotrophin (bHCG) expression in frozen cleavage embryo of In Vitro Fertilization (IVF) patients: An essential approach for better outcome towards human being inheritance in line with Maqasid Syari’ah concept
title_full Identification of Beta Human Chorionic Gonadotrophin (bHCG) expression in frozen cleavage embryo of In Vitro Fertilization (IVF) patients: An essential approach for better outcome towards human being inheritance in line with Maqasid Syari’ah concept
title_fullStr Identification of Beta Human Chorionic Gonadotrophin (bHCG) expression in frozen cleavage embryo of In Vitro Fertilization (IVF) patients: An essential approach for better outcome towards human being inheritance in line with Maqasid Syari’ah concept
title_full_unstemmed Identification of Beta Human Chorionic Gonadotrophin (bHCG) expression in frozen cleavage embryo of In Vitro Fertilization (IVF) patients: An essential approach for better outcome towards human being inheritance in line with Maqasid Syari’ah concept
title_sort identification of beta human chorionic gonadotrophin (bhcg) expression in frozen cleavage embryo of in vitro fertilization (ivf) patients: an essential approach for better outcome towards human being inheritance in line with maqasid syari’ah concept
publisher The International Medical Journal Malaysia
publishDate 2018
url http://irep.iium.edu.my/69829/1/Dr%20Roszaman-%20IMJM%20-NOA.pdf
http://irep.iium.edu.my/69829/
http://iiumedic.net/imjm/v1/download/volume_17_special_issue_2/Pages-from-2WCII-SI2-165.pdf
_version_ 1643618920214560768
score 13.211869

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