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A new isolate of thermophilic and organic solvent tolerant bacteria for lipase production using basal medium of palm kernel cake

Aims: This research focused on the selection of potential strains especially bacteria that can grow effectively in palm kernel cake (PKC) and produce high amount of thermostable and solvent tolerant (TS-OST) lipase. The work involved the exploration of renewable PKC as potential fermentation medium...

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Main Authors: Mohd Zubairi, Nurul Hidayah, Alam, Md Zahangir, Salleh, Mohd Noor, Mohd. Salleh, Hamzah, Fazil, Nurul Alia
Format: Article
Language:English
English
English
Published: Universiti Sains Malaysia 2018
Subjects:
TP Chemical technology
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/69116/1/69116_A%20new%20isolate%20of%20thermophilic.pdf
http://irep.iium.edu.my/69116/2/69116_A%20new%20isolate%20of%20thermophilic_SCOPUS.pdf
http://irep.iium.edu.my/69116/3/69116_A%20new%20isolate%20of%20thermophilic_WOS.pdf
http://irep.iium.edu.my/69116/
http://mjm.usm.my/uploads/issues/1383/MJM%202018-1-72.pdf
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spelling my.iium.irep.691162018-12-31T09:02:54Z http://irep.iium.edu.my/69116/ A new isolate of thermophilic and organic solvent tolerant bacteria for lipase production using basal medium of palm kernel cake Mohd Zubairi, Nurul Hidayah Alam, Md Zahangir Salleh, Mohd Noor Mohd. Salleh, Hamzah Fazil, Nurul Alia TP Chemical technology TP248.13 Biotechnology Aims: This research focused on the selection of potential strains especially bacteria that can grow effectively in palm kernel cake (PKC) and produce high amount of thermostable and solvent tolerant (TS-OST) lipase. The work involved the exploration of renewable PKC as potential fermentation medium for discovery to novel TS-OST lipase that would have excellent tolerance and activity in presence of organic solvents with high temperatures for industrial applications. Methodology and results: Using palm kernel cake (PKC) as source of thermophilic bacteria, 53 bacterial strains were found survived at temperature 65 °C. However, after subcultured several times, only 17 strains were found as pure thermophilic strains. Preliminary screening both qualitative and quantitative was performed to all 17 potential thermophilic bacterial strains and showed that only 11 purified thermophilic strains are lipase producer. Strain PKC-P1 produced highest enzyme activity (11.13 U/g), followed by PKC-P13 and PKC-C9. The lowest enzyme activity was lipase produced byPKC-C10 (0.76U/g). Strain PKC-P1 has been classified as Gram negative bacteria and identified as Bacillus smithii strain PKC_P1. Conclusion, significance and impact of study: PKC as a by-product of oil palm industry consists of many nutrients that can give benefits towards industry and can be utilized in order to produce enzymes like lipases. From these results, it could be concluded that this lipase stable at temperature 65 °C and pH 7 and may be a potential candidate to be used in a variety of biotechnological applications. This finding revealed that a bacterial strain obtained from oil-rich environment which is PKC through isolation process has potential as a source of more economical enzyme to be applied in biotechnology industry. © 2018. Universiti Sains Malaysia 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69116/1/69116_A%20new%20isolate%20of%20thermophilic.pdf application/pdf en http://irep.iium.edu.my/69116/2/69116_A%20new%20isolate%20of%20thermophilic_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/69116/3/69116_A%20new%20isolate%20of%20thermophilic_WOS.pdf Mohd Zubairi, Nurul Hidayah and Alam, Md Zahangir and Salleh, Mohd Noor and Mohd. Salleh, Hamzah and Fazil, Nurul Alia (2018) A new isolate of thermophilic and organic solvent tolerant bacteria for lipase production using basal medium of palm kernel cake. Malaysian Journal of Microbiology, 14 (2 (Special issue)). pp. 80-87. ISSN 1823-8262 http://mjm.usm.my/uploads/issues/1383/MJM%202018-1-72.pdf
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
English
topic TP Chemical technology
TP248.13 Biotechnology
spellingShingle TP Chemical technology
TP248.13 Biotechnology
Mohd Zubairi, Nurul Hidayah
Alam, Md Zahangir
Salleh, Mohd Noor
Mohd. Salleh, Hamzah
Fazil, Nurul Alia
A new isolate of thermophilic and organic solvent tolerant bacteria for lipase production using basal medium of palm kernel cake
description Aims: This research focused on the selection of potential strains especially bacteria that can grow effectively in palm kernel cake (PKC) and produce high amount of thermostable and solvent tolerant (TS-OST) lipase. The work involved the exploration of renewable PKC as potential fermentation medium for discovery to novel TS-OST lipase that would have excellent tolerance and activity in presence of organic solvents with high temperatures for industrial applications. Methodology and results: Using palm kernel cake (PKC) as source of thermophilic bacteria, 53 bacterial strains were found survived at temperature 65 °C. However, after subcultured several times, only 17 strains were found as pure thermophilic strains. Preliminary screening both qualitative and quantitative was performed to all 17 potential thermophilic bacterial strains and showed that only 11 purified thermophilic strains are lipase producer. Strain PKC-P1 produced highest enzyme activity (11.13 U/g), followed by PKC-P13 and PKC-C9. The lowest enzyme activity was lipase produced byPKC-C10 (0.76U/g). Strain PKC-P1 has been classified as Gram negative bacteria and identified as Bacillus smithii strain PKC_P1. Conclusion, significance and impact of study: PKC as a by-product of oil palm industry consists of many nutrients that can give benefits towards industry and can be utilized in order to produce enzymes like lipases. From these results, it could be concluded that this lipase stable at temperature 65 °C and pH 7 and may be a potential candidate to be used in a variety of biotechnological applications. This finding revealed that a bacterial strain obtained from oil-rich environment which is PKC through isolation process has potential as a source of more economical enzyme to be applied in biotechnology industry. © 2018.
format Article
author Mohd Zubairi, Nurul Hidayah
Alam, Md Zahangir
Salleh, Mohd Noor
Mohd. Salleh, Hamzah
Fazil, Nurul Alia
author_facet Mohd Zubairi, Nurul Hidayah
Alam, Md Zahangir
Salleh, Mohd Noor
Mohd. Salleh, Hamzah
Fazil, Nurul Alia
author_sort Mohd Zubairi, Nurul Hidayah
title A new isolate of thermophilic and organic solvent tolerant bacteria for lipase production using basal medium of palm kernel cake
title_short A new isolate of thermophilic and organic solvent tolerant bacteria for lipase production using basal medium of palm kernel cake
title_full A new isolate of thermophilic and organic solvent tolerant bacteria for lipase production using basal medium of palm kernel cake
title_fullStr A new isolate of thermophilic and organic solvent tolerant bacteria for lipase production using basal medium of palm kernel cake
title_full_unstemmed A new isolate of thermophilic and organic solvent tolerant bacteria for lipase production using basal medium of palm kernel cake
title_sort new isolate of thermophilic and organic solvent tolerant bacteria for lipase production using basal medium of palm kernel cake
publisher Universiti Sains Malaysia
publishDate 2018
url http://irep.iium.edu.my/69116/1/69116_A%20new%20isolate%20of%20thermophilic.pdf
http://irep.iium.edu.my/69116/2/69116_A%20new%20isolate%20of%20thermophilic_SCOPUS.pdf
http://irep.iium.edu.my/69116/3/69116_A%20new%20isolate%20of%20thermophilic_WOS.pdf
http://irep.iium.edu.my/69116/
http://mjm.usm.my/uploads/issues/1383/MJM%202018-1-72.pdf
_version_ 1643618718316494848
score 13.222552

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