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Evaluation of in vitro antidiabetic activity of selected flavonoids: structure activity relationship based study

Flavonoids have been reported to exert antihyperglycemic effect and have potential to enhance the current therapy options against type 2 diabetes mellitus. However, the structure activity relationships (SAR) studies of flavonoids against this disease have not been thoroughly understood. In the prese...

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Main Author: Ahmed, Qamar Uddin
Format: Conference or Workshop Item
Language:English
English
English
Published: 2018
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/69000/1/Dr%20Qamar%20U.%20Ahmed_PP_IMU%2015-16%20Aug%202018.pdf
http://irep.iium.edu.my/69000/2/Certificate%20of%20Presentation%20as%20an%20Invited%20Speaker.pdf
http://irep.iium.edu.my/69000/3/programme%20booklet_9August2018.pdf
http://irep.iium.edu.my/69000/
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spelling my.iium.irep.690002018-12-28T03:58:05Z http://irep.iium.edu.my/69000/ Evaluation of in vitro antidiabetic activity of selected flavonoids: structure activity relationship based study Ahmed, Qamar Uddin Q Science (General) Flavonoids have been reported to exert antihyperglycemic effect and have potential to enhance the current therapy options against type 2 diabetes mellitus. However, the structure activity relationships (SAR) studies of flavonoids against this disease have not been thoroughly understood. In the present study, fourteen structurally related flavonoids were taken into account for in vitro antidiabetic evaluation. Cell viability of RIN-5F pancreatic cells and 3T3-L1 pre-adipocyte cells were initially tested, then, insulin secretion assay of RIN-5F as well as adipogenesis, and glucose uptake measurement of adipocyte were investigated. Subsequently, protein expression study through adipokines measurement, western blotting analysis against GLUT4 and C/EBP-α was carried out. The results from insulin secretion, adipogenesis, glucose uptake and protein expression assays revealed that the methoxy group at position C-8 is responsible for antidiabetic property of selected flavonoids via glucose uptake mechanism indicated by up regulation of GLUT4 and C/EBP-α expressions. The mechanism was seen to be enhanced by the addition of acetate group at C-5 and C-7. Further investigation is still necessary to clarify biological outcomes and activities of flavonoids, to distinguish their metabolites with special functional groups, thus meeting therapeutic and nutritional requirements. 2018-08-15 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/69000/1/Dr%20Qamar%20U.%20Ahmed_PP_IMU%2015-16%20Aug%202018.pdf application/pdf en http://irep.iium.edu.my/69000/2/Certificate%20of%20Presentation%20as%20an%20Invited%20Speaker.pdf application/pdf en http://irep.iium.edu.my/69000/3/programme%20booklet_9August2018.pdf Ahmed, Qamar Uddin (2018) Evaluation of in vitro antidiabetic activity of selected flavonoids: structure activity relationship based study. In: 6th International Postgraduate Conference on Pharmaceutical Sciences 2018, 15th-16th August 2018, International Medical University, Kuala Lumpur, Malaysia. (Unpublished)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
English
topic Q Science (General)
spellingShingle Q Science (General)
Ahmed, Qamar Uddin
Evaluation of in vitro antidiabetic activity of selected flavonoids: structure activity relationship based study
description Flavonoids have been reported to exert antihyperglycemic effect and have potential to enhance the current therapy options against type 2 diabetes mellitus. However, the structure activity relationships (SAR) studies of flavonoids against this disease have not been thoroughly understood. In the present study, fourteen structurally related flavonoids were taken into account for in vitro antidiabetic evaluation. Cell viability of RIN-5F pancreatic cells and 3T3-L1 pre-adipocyte cells were initially tested, then, insulin secretion assay of RIN-5F as well as adipogenesis, and glucose uptake measurement of adipocyte were investigated. Subsequently, protein expression study through adipokines measurement, western blotting analysis against GLUT4 and C/EBP-α was carried out. The results from insulin secretion, adipogenesis, glucose uptake and protein expression assays revealed that the methoxy group at position C-8 is responsible for antidiabetic property of selected flavonoids via glucose uptake mechanism indicated by up regulation of GLUT4 and C/EBP-α expressions. The mechanism was seen to be enhanced by the addition of acetate group at C-5 and C-7. Further investigation is still necessary to clarify biological outcomes and activities of flavonoids, to distinguish their metabolites with special functional groups, thus meeting therapeutic and nutritional requirements.
format Conference or Workshop Item
author Ahmed, Qamar Uddin
author_facet Ahmed, Qamar Uddin
author_sort Ahmed, Qamar Uddin
title Evaluation of in vitro antidiabetic activity of selected flavonoids: structure activity relationship based study
title_short Evaluation of in vitro antidiabetic activity of selected flavonoids: structure activity relationship based study
title_full Evaluation of in vitro antidiabetic activity of selected flavonoids: structure activity relationship based study
title_fullStr Evaluation of in vitro antidiabetic activity of selected flavonoids: structure activity relationship based study
title_full_unstemmed Evaluation of in vitro antidiabetic activity of selected flavonoids: structure activity relationship based study
title_sort evaluation of in vitro antidiabetic activity of selected flavonoids: structure activity relationship based study
publishDate 2018
url http://irep.iium.edu.my/69000/1/Dr%20Qamar%20U.%20Ahmed_PP_IMU%2015-16%20Aug%202018.pdf
http://irep.iium.edu.my/69000/2/Certificate%20of%20Presentation%20as%20an%20Invited%20Speaker.pdf
http://irep.iium.edu.my/69000/3/programme%20booklet_9August2018.pdf
http://irep.iium.edu.my/69000/
_version_ 1643618695410352128
score 13.211869

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