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Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya

Introduction: Leaves of mempelas paya (Tetracera indica) (family: Dilleniaceae) is traditionally used in diabetes management in Malaysia. However, no attempt has ever been made to discover an active principle responsible for its blood glucose lowering effect. Hence, this...

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Main Authors: Ahmed, Qamar Uddin, Mat So'ad, Siti Zaiton, Afrin, Mehnaz, Wan Sulaiman, Wan Mohd. Azizi
Format: Article
Language:English
Published: International Islamic University Malaysia 2016
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/68954/1/2016_IMJM_MRS2016-ABSTRACT-BOOK-41.pdf
http://irep.iium.edu.my/68954/
http://iiumedic.net/imjm/v1/download/Volume%2015%20Supplement/MRS2016-ABSTRACT-BOOK-41.pdf
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spelling my.iium.irep.689542019-01-02T04:50:09Z http://irep.iium.edu.my/68954/ Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya Ahmed, Qamar Uddin Mat So'ad, Siti Zaiton Afrin, Mehnaz Wan Sulaiman, Wan Mohd. Azizi Q Science (General) Introduction: Leaves of mempelas paya (Tetracera indica) (family: Dilleniaceae) is traditionally used in diabetes management in Malaysia. However, no attempt has ever been made to discover an active principle responsible for its blood glucose lowering effect. Hence, this research deals with the isolation, in vivo antidiabetic and toxicological evaluation of an active principle. Methods: Chemical investigation of methanol extract derived from the powdered leaves resulted in the isolation of a phenolic compound through repeated silica gel column chromatography. The structure was elucidated by spectroscopic (1H-NMR, 13C -NMR, MS, IR and UV) analysis. In vivo antidiabetic and toxicological studies were carried out using Sprague -Dawley rats (diabetic and normal) at three different concentrations (1, 5 and 25 mg/kg b.w.). Results: 5,7-dihydroxy-8-methoxyflavone was found to exhibit significant anti-hyperglycaemic activity (p<0.05) in diabetic rats at 5 and 25 mg/kg b.w. without showing hypoglycaemic effect in normal rats. The anti-hyperglycaemic activity was found to be comparable with glibenclamide (GLBC) (p<0.05). In vivo toxicological study for continuous 21 days showed no abnormal activity and mortality. Histopathology of kidney, liver and pancreas of both normal and diabetic treated rats demonstrated normal and improved condition when compared to diabetic control group. There were no significant changes seen in the kidney (serum urea and creatinine) and lipid (TC, TG, LDL-C and HDL-C) functional parameters tested. Conclusions: 5,7-dihydroxy-8-methoxyflavone isolated from the leaves of mempelas paya coud be a safe antidiabetic agent. International Islamic University Malaysia 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/68954/1/2016_IMJM_MRS2016-ABSTRACT-BOOK-41.pdf Ahmed, Qamar Uddin and Mat So'ad, Siti Zaiton and Afrin, Mehnaz and Wan Sulaiman, Wan Mohd. Azizi (2016) Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya. International Medical Journal Malaysia, 15 (Supplement). p. 41. ISSN 1823-4631 http://iiumedic.net/imjm/v1/download/Volume%2015%20Supplement/MRS2016-ABSTRACT-BOOK-41.pdf
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Ahmed, Qamar Uddin
Mat So'ad, Siti Zaiton
Afrin, Mehnaz
Wan Sulaiman, Wan Mohd. Azizi
Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
description Introduction: Leaves of mempelas paya (Tetracera indica) (family: Dilleniaceae) is traditionally used in diabetes management in Malaysia. However, no attempt has ever been made to discover an active principle responsible for its blood glucose lowering effect. Hence, this research deals with the isolation, in vivo antidiabetic and toxicological evaluation of an active principle. Methods: Chemical investigation of methanol extract derived from the powdered leaves resulted in the isolation of a phenolic compound through repeated silica gel column chromatography. The structure was elucidated by spectroscopic (1H-NMR, 13C -NMR, MS, IR and UV) analysis. In vivo antidiabetic and toxicological studies were carried out using Sprague -Dawley rats (diabetic and normal) at three different concentrations (1, 5 and 25 mg/kg b.w.). Results: 5,7-dihydroxy-8-methoxyflavone was found to exhibit significant anti-hyperglycaemic activity (p<0.05) in diabetic rats at 5 and 25 mg/kg b.w. without showing hypoglycaemic effect in normal rats. The anti-hyperglycaemic activity was found to be comparable with glibenclamide (GLBC) (p<0.05). In vivo toxicological study for continuous 21 days showed no abnormal activity and mortality. Histopathology of kidney, liver and pancreas of both normal and diabetic treated rats demonstrated normal and improved condition when compared to diabetic control group. There were no significant changes seen in the kidney (serum urea and creatinine) and lipid (TC, TG, LDL-C and HDL-C) functional parameters tested. Conclusions: 5,7-dihydroxy-8-methoxyflavone isolated from the leaves of mempelas paya coud be a safe antidiabetic agent.
format Article
author Ahmed, Qamar Uddin
Mat So'ad, Siti Zaiton
Afrin, Mehnaz
Wan Sulaiman, Wan Mohd. Azizi
author_facet Ahmed, Qamar Uddin
Mat So'ad, Siti Zaiton
Afrin, Mehnaz
Wan Sulaiman, Wan Mohd. Azizi
author_sort Ahmed, Qamar Uddin
title Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
title_short Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
title_full Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
title_fullStr Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
title_full_unstemmed Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
title_sort investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
publisher International Islamic University Malaysia
publishDate 2016
url http://irep.iium.edu.my/68954/1/2016_IMJM_MRS2016-ABSTRACT-BOOK-41.pdf
http://irep.iium.edu.my/68954/
http://iiumedic.net/imjm/v1/download/Volume%2015%20Supplement/MRS2016-ABSTRACT-BOOK-41.pdf
_version_ 1643618687774621696
score 13.211869

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