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Graveoline from Ruta angustifolia (L.) Pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations = Graveolin daripada Ruta angustifolia (L.) Pers. dan potensi sinergistik antimikrobnya dalam gabungan erithromicin atau vancomicin

Ruta angustifolia (L.) Pers. is a Rutaceous species which contains various anthranilic acid derived alkaloids including the bioactive quinolones. This study is aimed at identifying the antimicrobial active alkaloids of R. angustifolia and evaluating their potential as synergistic enhancers in alkalo...

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Main Authors: Mohd Kamal, Laina Zarisa, Mohd Hassan, Norazian, Md Taib, Nurhaya, Khin Soe, May
Format: Article
Language:English
English
Published: Penerbit Universiti Kebangsaan Malaysia (UKM) 2018
Subjects:
RM300 Drugs and their action
RS403 Materia Medica-Pharmaceutical Chemistry
Online Access:http://irep.iium.edu.my/68144/1/68144_Graveoline%20from%20Ruta%20angustifolia%20%28L.%29%20Pers..pdf
http://irep.iium.edu.my/68144/2/68144_Graveoline%20from%20Ruta%20angustifolia%20%28L.%29%20Pers._WOS.pdf
http://irep.iium.edu.my/68144/
http://www.ukm.my/jsm/pdf_files/SM-PDF-47-10-2018/19%20Laina%20Zarisa%20Mohd%20Kamal.pdf
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id my.iium.irep.68144
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institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic RM300 Drugs and their action
RS403 Materia Medica-Pharmaceutical Chemistry
spellingShingle RM300 Drugs and their action
RS403 Materia Medica-Pharmaceutical Chemistry
Mohd Kamal, Laina Zarisa
Mohd Hassan, Norazian
Md Taib, Nurhaya
Khin Soe, May
Graveoline from Ruta angustifolia (L.) Pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations = Graveolin daripada Ruta angustifolia (L.) Pers. dan potensi sinergistik antimikrobnya dalam gabungan erithromicin atau vancomicin
description Ruta angustifolia (L.) Pers. is a Rutaceous species which contains various anthranilic acid derived alkaloids including the bioactive quinolones. This study is aimed at identifying the antimicrobial active alkaloids of R. angustifolia and evaluating their potential as synergistic enhancers in alkaloid-antibiotic combinations. Antimicrobial bioautography- guided isolation of alkaloidal fractions of R. angustifolia leaves has led to the identification of 2,3-dimethoxy-1-hydroxy- 10-methylacridone [arborinine]; and 4,7,8-trimethoxyfuro[2,3-b]quinoline [skimmianine]; together with the major active alkaloid, 1-methyl-2-[3’,4’-methylenedioxyphenyl]-4-quinolone [graveoline]. Graveoline showed Minimum Inhibitory Concentration (MIC) values ranging from 500 to 1000 μg/mL against Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212 and Escherichia coli ATCC 25922. Checkerboard assay for antimicrobial combination effects between graveoline with either erythromycin or vancomycin showed enhancement of the antimicrobial activity of both antibiotics with Fractional Inhibitory Concentration Indices (FICI) ranged from 0.37 to 1.50. Synergistic effect with FICI of 0.37 was observed for graveoline-erythromycin combination against S. aureus compared to FICI of 1.00 for ciprofloxacin-erythromycin additive effect. Graveoline was a potential candidate for antimicrobial combination agent especially against S. aureus. The result supports the idea of using plant metabolites as antimicrobial synergistic agents. ********************************************************* Ruta angustifolia (L.) Pers. adalah spesies Rutaceae yang mengandungi pelbagai alkaloid yang hasilkan daripada asid anthranilik seperti alkaloid quinolon. Kajian ini bertujuan untuk mengenal pasti alkaloid aktif daripada R. angustifolia dan potensinya sebagai penggalak kesan sinergi bersama antibiotik terpilih. Pemencilan alkaloid aktif berpandukan bioautografi antimikrob daripada pecahan ekstrak daun R. angustifolia berjaya mengenal pasti 2,3-dimetoksi-1-hidroksi-10-metilakridon [arborinin]; dan 4,7,8-trimetoksifuro[2,3-b]quinolin [skimmianin]; dan akaloid aktif utama 1-metil-2-[3’,4’-metilenedioksifenil]-4-quinolon [graveolin]. Graveolin menunjukkan Kepekatan Perencatan Minima (MIC) pada julat antara 500 dan 1000 μg/mL terhadap Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212 dan Escherichia coli ATCC 25922. Gabungan antara graveolin dan erithromicin atau vankomicin yang dinilai melalui Assay Checkerboard menunjukkan bahawa graveolin meningkatkan aktiviti kedua-dua antibiotik dengan Indeks Pecahan Kepekatan Perencatan (FICI) antara 0.37 dan 1.50. Kesan sinergi dengan nilai FICI 0.37 ditunjukkan oleh gabungan graveolin-erithromicin terhadap S. aureus berbanding kesan tambahan dengan nilai FICI 1.00 oleh gabungan ciprofloxacin-erithromicin. Graveolin mempunyai potensi sebagai agen gabungan antimikrob terutama terhadap S. aureus. Keputusan kajian ini menyokong penggunaan metabolit daripada tumbuhan sebagai agen sinergi antimikrob.
format Article
author Mohd Kamal, Laina Zarisa
Mohd Hassan, Norazian
Md Taib, Nurhaya
Khin Soe, May
author_facet Mohd Kamal, Laina Zarisa
Mohd Hassan, Norazian
Md Taib, Nurhaya
Khin Soe, May
author_sort Mohd Kamal, Laina Zarisa
title Graveoline from Ruta angustifolia (L.) Pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations = Graveolin daripada Ruta angustifolia (L.) Pers. dan potensi sinergistik antimikrobnya dalam gabungan erithromicin atau vancomicin
title_short Graveoline from Ruta angustifolia (L.) Pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations = Graveolin daripada Ruta angustifolia (L.) Pers. dan potensi sinergistik antimikrobnya dalam gabungan erithromicin atau vancomicin
title_full Graveoline from Ruta angustifolia (L.) Pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations = Graveolin daripada Ruta angustifolia (L.) Pers. dan potensi sinergistik antimikrobnya dalam gabungan erithromicin atau vancomicin
title_fullStr Graveoline from Ruta angustifolia (L.) Pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations = Graveolin daripada Ruta angustifolia (L.) Pers. dan potensi sinergistik antimikrobnya dalam gabungan erithromicin atau vancomicin
title_full_unstemmed Graveoline from Ruta angustifolia (L.) Pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations = Graveolin daripada Ruta angustifolia (L.) Pers. dan potensi sinergistik antimikrobnya dalam gabungan erithromicin atau vancomicin
title_sort graveoline from ruta angustifolia (l.) pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations = graveolin daripada ruta angustifolia (l.) pers. dan potensi sinergistik antimikrobnya dalam gabungan erithromicin atau vancomicin
publisher Penerbit Universiti Kebangsaan Malaysia (UKM)
publishDate 2018
url http://irep.iium.edu.my/68144/1/68144_Graveoline%20from%20Ruta%20angustifolia%20%28L.%29%20Pers..pdf
http://irep.iium.edu.my/68144/2/68144_Graveoline%20from%20Ruta%20angustifolia%20%28L.%29%20Pers._WOS.pdf
http://irep.iium.edu.my/68144/
http://www.ukm.my/jsm/pdf_files/SM-PDF-47-10-2018/19%20Laina%20Zarisa%20Mohd%20Kamal.pdf
_version_ 1643618348742737920
spelling my.iium.irep.681442018-12-14T09:22:39Z http://irep.iium.edu.my/68144/ Graveoline from Ruta angustifolia (L.) Pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations = Graveolin daripada Ruta angustifolia (L.) Pers. dan potensi sinergistik antimikrobnya dalam gabungan erithromicin atau vancomicin Mohd Kamal, Laina Zarisa Mohd Hassan, Norazian Md Taib, Nurhaya Khin Soe, May RM300 Drugs and their action RS403 Materia Medica-Pharmaceutical Chemistry Ruta angustifolia (L.) Pers. is a Rutaceous species which contains various anthranilic acid derived alkaloids including the bioactive quinolones. This study is aimed at identifying the antimicrobial active alkaloids of R. angustifolia and evaluating their potential as synergistic enhancers in alkaloid-antibiotic combinations. Antimicrobial bioautography- guided isolation of alkaloidal fractions of R. angustifolia leaves has led to the identification of 2,3-dimethoxy-1-hydroxy- 10-methylacridone [arborinine]; and 4,7,8-trimethoxyfuro[2,3-b]quinoline [skimmianine]; together with the major active alkaloid, 1-methyl-2-[3’,4’-methylenedioxyphenyl]-4-quinolone [graveoline]. Graveoline showed Minimum Inhibitory Concentration (MIC) values ranging from 500 to 1000 μg/mL against Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212 and Escherichia coli ATCC 25922. Checkerboard assay for antimicrobial combination effects between graveoline with either erythromycin or vancomycin showed enhancement of the antimicrobial activity of both antibiotics with Fractional Inhibitory Concentration Indices (FICI) ranged from 0.37 to 1.50. Synergistic effect with FICI of 0.37 was observed for graveoline-erythromycin combination against S. aureus compared to FICI of 1.00 for ciprofloxacin-erythromycin additive effect. Graveoline was a potential candidate for antimicrobial combination agent especially against S. aureus. The result supports the idea of using plant metabolites as antimicrobial synergistic agents. ********************************************************* Ruta angustifolia (L.) Pers. adalah spesies Rutaceae yang mengandungi pelbagai alkaloid yang hasilkan daripada asid anthranilik seperti alkaloid quinolon. Kajian ini bertujuan untuk mengenal pasti alkaloid aktif daripada R. angustifolia dan potensinya sebagai penggalak kesan sinergi bersama antibiotik terpilih. Pemencilan alkaloid aktif berpandukan bioautografi antimikrob daripada pecahan ekstrak daun R. angustifolia berjaya mengenal pasti 2,3-dimetoksi-1-hidroksi-10-metilakridon [arborinin]; dan 4,7,8-trimetoksifuro[2,3-b]quinolin [skimmianin]; dan akaloid aktif utama 1-metil-2-[3’,4’-metilenedioksifenil]-4-quinolon [graveolin]. Graveolin menunjukkan Kepekatan Perencatan Minima (MIC) pada julat antara 500 dan 1000 μg/mL terhadap Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212 dan Escherichia coli ATCC 25922. Gabungan antara graveolin dan erithromicin atau vankomicin yang dinilai melalui Assay Checkerboard menunjukkan bahawa graveolin meningkatkan aktiviti kedua-dua antibiotik dengan Indeks Pecahan Kepekatan Perencatan (FICI) antara 0.37 dan 1.50. Kesan sinergi dengan nilai FICI 0.37 ditunjukkan oleh gabungan graveolin-erithromicin terhadap S. aureus berbanding kesan tambahan dengan nilai FICI 1.00 oleh gabungan ciprofloxacin-erithromicin. Graveolin mempunyai potensi sebagai agen gabungan antimikrob terutama terhadap S. aureus. Keputusan kajian ini menyokong penggunaan metabolit daripada tumbuhan sebagai agen sinergi antimikrob. Penerbit Universiti Kebangsaan Malaysia (UKM) 2018-11-29 Article PeerReviewed application/pdf en http://irep.iium.edu.my/68144/1/68144_Graveoline%20from%20Ruta%20angustifolia%20%28L.%29%20Pers..pdf application/pdf en http://irep.iium.edu.my/68144/2/68144_Graveoline%20from%20Ruta%20angustifolia%20%28L.%29%20Pers._WOS.pdf Mohd Kamal, Laina Zarisa and Mohd Hassan, Norazian and Md Taib, Nurhaya and Khin Soe, May (2018) Graveoline from Ruta angustifolia (L.) Pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations = Graveolin daripada Ruta angustifolia (L.) Pers. dan potensi sinergistik antimikrobnya dalam gabungan erithromicin atau vancomicin. Sains Malaysiana, 47 (10). pp. 2429-2435. ISSN 0126-6039 http://www.ukm.my/jsm/pdf_files/SM-PDF-47-10-2018/19%20Laina%20Zarisa%20Mohd%20Kamal.pdf 10.17576/jsm-2018-4710-19
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