Cover Image

Screening of parameters for solid-state fermentation of Aspergillus niger producing cellulose using sugarcane bagasse as substrate

Microfibrillated cellulose (MFC) is a promising biomaterial in bionanocomposites production because of its renewable and eco-friendly properties. Conventional methods to produce MFC such as acid hydrolysis and mechanical treatment posed significant drawbacks; large amount of acid is required and incr...

Full description

Saved in:
Bibliographic Details
Main Authors: Jimat, Dzun Noraini, Salleh, Norhidayah, Jami, Mohammed Saedi
Format: Article
Language:English
Published: IIUM Press, International Islamic University Malaysia 2018
Subjects:
TP Chemical technology
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/67996/1/67996_Screening%20of%20parameters%20for%20solid-state.pdf
http://irep.iium.edu.my/67996/
http://journals.iium.edu.my/bnrej/index.php/bnrej/article/view/21/4
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.iium.irep.67996
record_format dspace
spelling my.iium.irep.679962019-07-11T02:20:37Z http://irep.iium.edu.my/67996/ Screening of parameters for solid-state fermentation of Aspergillus niger producing cellulose using sugarcane bagasse as substrate Jimat, Dzun Noraini Salleh, Norhidayah Jami, Mohammed Saedi TP Chemical technology TP248.13 Biotechnology Microfibrillated cellulose (MFC) is a promising biomaterial in bionanocomposites production because of its renewable and eco-friendly properties. Conventional methods to produce MFC such as acid hydrolysis and mechanical treatment posed significant drawbacks; large amount of acid is required and increased defibrillation of alpha cellulose, respectively. Therefore, an appropriate method is very important in obtaining desired features of MFC such as high mechanical strength, high surface and low density properties. The utilization of agro-industry wastes such as sugarcane bagasse for MFC production could help to reduce the problem of lignocellulosic wastes. Thus, this study aimed to treat the sugarcane bagasse using fungal cellulases hydrolysis as a pretreatment method thus assisting the fibrillation of fiber cellulose via ultrasonication. The effects of solid-state fermentation of Aspergillus niger on cellulases activities and morphological changes of sugarcane bagasse (SCB) were investigated. Variables such as sugarcane bagasse particle size (400 and 600 µm), inoculum size (2% and 5%), medium pH (5 and 7), and fermentation time (5 and 15 days) were screened using 2-level factorial design (Design expert software, Stat-Ease Inc., Version 8.0). Analysis of variance (ANOVA) showed that the most significant parameter that affected the structural changes of sugarcane bagasse was its particle size. The increased glucose production indicated the increased of defibrillation of sugarcane bagasse. The use of fungal pretreatment through solid state fermentation of Aspergillus niger prior to ultrasonication was found to assist the later process in fibrillating sugarcane fibres. The results obtained from enzyme assays, optical microscope, SEM and FTIR showed the supporting evidence about the successful fibrillation cellulose of SCB. IIUM Press, International Islamic University Malaysia 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/67996/1/67996_Screening%20of%20parameters%20for%20solid-state.pdf Jimat, Dzun Noraini and Salleh, Norhidayah and Jami, Mohammed Saedi (2018) Screening of parameters for solid-state fermentation of Aspergillus niger producing cellulose using sugarcane bagasse as substrate. Biological and Natural Resources Engineering Journal, 1 (1). pp. 26-35. http://journals.iium.edu.my/bnrej/index.php/bnrej/article/view/21/4
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP Chemical technology
TP248.13 Biotechnology
spellingShingle TP Chemical technology
TP248.13 Biotechnology
Jimat, Dzun Noraini
Salleh, Norhidayah
Jami, Mohammed Saedi
Screening of parameters for solid-state fermentation of Aspergillus niger producing cellulose using sugarcane bagasse as substrate
description Microfibrillated cellulose (MFC) is a promising biomaterial in bionanocomposites production because of its renewable and eco-friendly properties. Conventional methods to produce MFC such as acid hydrolysis and mechanical treatment posed significant drawbacks; large amount of acid is required and increased defibrillation of alpha cellulose, respectively. Therefore, an appropriate method is very important in obtaining desired features of MFC such as high mechanical strength, high surface and low density properties. The utilization of agro-industry wastes such as sugarcane bagasse for MFC production could help to reduce the problem of lignocellulosic wastes. Thus, this study aimed to treat the sugarcane bagasse using fungal cellulases hydrolysis as a pretreatment method thus assisting the fibrillation of fiber cellulose via ultrasonication. The effects of solid-state fermentation of Aspergillus niger on cellulases activities and morphological changes of sugarcane bagasse (SCB) were investigated. Variables such as sugarcane bagasse particle size (400 and 600 µm), inoculum size (2% and 5%), medium pH (5 and 7), and fermentation time (5 and 15 days) were screened using 2-level factorial design (Design expert software, Stat-Ease Inc., Version 8.0). Analysis of variance (ANOVA) showed that the most significant parameter that affected the structural changes of sugarcane bagasse was its particle size. The increased glucose production indicated the increased of defibrillation of sugarcane bagasse. The use of fungal pretreatment through solid state fermentation of Aspergillus niger prior to ultrasonication was found to assist the later process in fibrillating sugarcane fibres. The results obtained from enzyme assays, optical microscope, SEM and FTIR showed the supporting evidence about the successful fibrillation cellulose of SCB.
format Article
author Jimat, Dzun Noraini
Salleh, Norhidayah
Jami, Mohammed Saedi
author_facet Jimat, Dzun Noraini
Salleh, Norhidayah
Jami, Mohammed Saedi
author_sort Jimat, Dzun Noraini
title Screening of parameters for solid-state fermentation of Aspergillus niger producing cellulose using sugarcane bagasse as substrate
title_short Screening of parameters for solid-state fermentation of Aspergillus niger producing cellulose using sugarcane bagasse as substrate
title_full Screening of parameters for solid-state fermentation of Aspergillus niger producing cellulose using sugarcane bagasse as substrate
title_fullStr Screening of parameters for solid-state fermentation of Aspergillus niger producing cellulose using sugarcane bagasse as substrate
title_full_unstemmed Screening of parameters for solid-state fermentation of Aspergillus niger producing cellulose using sugarcane bagasse as substrate
title_sort screening of parameters for solid-state fermentation of aspergillus niger producing cellulose using sugarcane bagasse as substrate
publisher IIUM Press, International Islamic University Malaysia
publishDate 2018
url http://irep.iium.edu.my/67996/1/67996_Screening%20of%20parameters%20for%20solid-state.pdf
http://irep.iium.edu.my/67996/
http://journals.iium.edu.my/bnrej/index.php/bnrej/article/view/21/4
_version_ 1643619784302002176
score 13.211869

Similar Items