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Shedding light on lipase stability in natural deep eutectic solvents

This study presents the potential role of natural deep eutectic solvents (NADESs) in a lipase-catalyzed hydrolysis reaction as both a co-solvent in an aqueous solution and as a main solvent. Ammonium salts such as choline chloride (ChCl) were paired with different hydrogen bond donors such as gly...

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Main Authors: Elgharbawy, Amal Ahmad, Hayyan, Adeeb, Hayyan, Maan, Rashid, Shahidah Nusailah, Nor, M.R.M., Zulkifli, M.Y., Alias, Yatimah, Mirghani, Mohamed Elwathig Saeed
Format: Article
Language:English
English
Published: Croation Society of Chemical Engineers 2018
Subjects:
QD Chemistry
T Technology (General)
Online Access:http://irep.iium.edu.my/67015/1/elgharbawy%20et%20al%202018.pdf
http://irep.iium.edu.my/67015/7/67015_Shedding%20light%20on%20lipase%20stability%20in%20natural%20_scopus.pdf
http://irep.iium.edu.my/67015/
http://silverstripe.fkit.hr/cabeq/past-issues/article/1515
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spelling my.iium.irep.670152019-03-14T03:41:24Z http://irep.iium.edu.my/67015/ Shedding light on lipase stability in natural deep eutectic solvents Elgharbawy, Amal Ahmad Hayyan, Adeeb Hayyan, Maan Rashid, Shahidah Nusailah Nor, M.R.M. Zulkifli, M.Y. Alias, Yatimah Mirghani, Mohamed Elwathig Saeed QD Chemistry T Technology (General) This study presents the potential role of natural deep eutectic solvents (NADESs) in a lipase-catalyzed hydrolysis reaction as both a co-solvent in an aqueous solution and as a main solvent. Ammonium salts such as choline chloride (ChCl) were paired with different hydrogen bond donors such as glycerol and malonic acid and sugars (glucose, fructose and sucrose). The hydrolysis of p-nitrophenyl palmitate by six different lipases: lipase from porcine pancreas (PR), lipase from Candida rugosa (CR), Amano lipase PS, from Burkholderia cepacia (AM), lipase from Rhizopus niveus (RN), lipase acrylic resin from Candida antartica (ARC), lipase B Candida antartica immobilized on Immobead 150, recombinant from Aspergillus oryzae (CALB), were tested in five NADESs. The results showed that NADES3 prepared from ChCl/sucrose was the most promising solvent as it enhanced the activities of both CALB and lipase from porcine pancreas to 355 % and 345 %. The kinetics investigation confirmed the higher catalytic efficiency (kcat/Km) of lipases in the 40 % of (NADES3) and compared with the aqueous form. The trend achieved by NADES may be a promising approach for applications and further perspectives as genuinely green industrial solvents. Croation Society of Chemical Engineers 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/67015/1/elgharbawy%20et%20al%202018.pdf application/pdf en http://irep.iium.edu.my/67015/7/67015_Shedding%20light%20on%20lipase%20stability%20in%20natural%20_scopus.pdf Elgharbawy, Amal Ahmad and Hayyan, Adeeb and Hayyan, Maan and Rashid, Shahidah Nusailah and Nor, M.R.M. and Zulkifli, M.Y. and Alias, Yatimah and Mirghani, Mohamed Elwathig Saeed (2018) Shedding light on lipase stability in natural deep eutectic solvents. Chemical and Biochemical Engineering Quarterly, 32 (3). pp. 359-370. ISSN 0352-9568 http://silverstripe.fkit.hr/cabeq/past-issues/article/1515 10.15255/CABEQ.2018.1335
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic QD Chemistry
T Technology (General)
spellingShingle QD Chemistry
T Technology (General)
Elgharbawy, Amal Ahmad
Hayyan, Adeeb
Hayyan, Maan
Rashid, Shahidah Nusailah
Nor, M.R.M.
Zulkifli, M.Y.
Alias, Yatimah
Mirghani, Mohamed Elwathig Saeed
Shedding light on lipase stability in natural deep eutectic solvents
description This study presents the potential role of natural deep eutectic solvents (NADESs) in a lipase-catalyzed hydrolysis reaction as both a co-solvent in an aqueous solution and as a main solvent. Ammonium salts such as choline chloride (ChCl) were paired with different hydrogen bond donors such as glycerol and malonic acid and sugars (glucose, fructose and sucrose). The hydrolysis of p-nitrophenyl palmitate by six different lipases: lipase from porcine pancreas (PR), lipase from Candida rugosa (CR), Amano lipase PS, from Burkholderia cepacia (AM), lipase from Rhizopus niveus (RN), lipase acrylic resin from Candida antartica (ARC), lipase B Candida antartica immobilized on Immobead 150, recombinant from Aspergillus oryzae (CALB), were tested in five NADESs. The results showed that NADES3 prepared from ChCl/sucrose was the most promising solvent as it enhanced the activities of both CALB and lipase from porcine pancreas to 355 % and 345 %. The kinetics investigation confirmed the higher catalytic efficiency (kcat/Km) of lipases in the 40 % of (NADES3) and compared with the aqueous form. The trend achieved by NADES may be a promising approach for applications and further perspectives as genuinely green industrial solvents.
format Article
author Elgharbawy, Amal Ahmad
Hayyan, Adeeb
Hayyan, Maan
Rashid, Shahidah Nusailah
Nor, M.R.M.
Zulkifli, M.Y.
Alias, Yatimah
Mirghani, Mohamed Elwathig Saeed
author_facet Elgharbawy, Amal Ahmad
Hayyan, Adeeb
Hayyan, Maan
Rashid, Shahidah Nusailah
Nor, M.R.M.
Zulkifli, M.Y.
Alias, Yatimah
Mirghani, Mohamed Elwathig Saeed
author_sort Elgharbawy, Amal Ahmad
title Shedding light on lipase stability in natural deep eutectic solvents
title_short Shedding light on lipase stability in natural deep eutectic solvents
title_full Shedding light on lipase stability in natural deep eutectic solvents
title_fullStr Shedding light on lipase stability in natural deep eutectic solvents
title_full_unstemmed Shedding light on lipase stability in natural deep eutectic solvents
title_sort shedding light on lipase stability in natural deep eutectic solvents
publisher Croation Society of Chemical Engineers
publishDate 2018
url http://irep.iium.edu.my/67015/1/elgharbawy%20et%20al%202018.pdf
http://irep.iium.edu.my/67015/7/67015_Shedding%20light%20on%20lipase%20stability%20in%20natural%20_scopus.pdf
http://irep.iium.edu.my/67015/
http://silverstripe.fkit.hr/cabeq/past-issues/article/1515
_version_ 1643619369623748608
score 13.211869

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