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The role of poly(ADP-ribose) polymerase-1 inhibitor in carrageenan-induced lung inflammation in mice.

Increasing indication is unveiling a role for poly(ADP-ribose) polymerase (PARP)-1 in the regulation of inflammatory/immune responses. The aim of the present study was to determine the potential anti-inflammatory effects of PARP-1 inhibitor 5-aminoisoquinolinone (5-AIQ) to explore the role of PARP-1...

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Main Authors: Ahmada, Sheikh Fayaz, Zoheir, Khairy M.A., Ansari, Mushtaq Ahmad, Korashya, Hesham M., Bakheet, Saleh A., Ashour, Abdelkader Elbadawy Abbas, Al-Shabanaha, Othman A., Al-harbi, Mohammed M., Attiaa, Sabry M.
Format: Article
Language:English
English
Published: Elsevier 2015
Subjects:
RB Pathology
Online Access:http://irep.iium.edu.my/66953/1/The%20role%20of%20poly%28ADP-ribose%29%20polymerase-1%20inhibitor%20in%20carrageenan-induced%20lung%20inflammation%20in%20mice..pdf
http://irep.iium.edu.my/66953/7/66953_Scopus%20-%20Document%20details.pdf
http://irep.iium.edu.my/66953/
https://www.journals.elsevier.com/molecular-immunology
https://doi.org/10.1016/j.molimm.2014.09.009
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spelling my.iium.irep.669532019-01-06T07:43:46Z http://irep.iium.edu.my/66953/ The role of poly(ADP-ribose) polymerase-1 inhibitor in carrageenan-induced lung inflammation in mice. Ahmada, Sheikh Fayaz Zoheir, Khairy M.A. Ansari, Mushtaq Ahmad Korashya, Hesham M. Bakheet, Saleh A. Ashour, Abdelkader Elbadawy Abbas Al-Shabanaha, Othman A. Al-harbi, Mohammed M. Attiaa, Sabry M. RB Pathology Increasing indication is unveiling a role for poly(ADP-ribose) polymerase (PARP)-1 in the regulation of inflammatory/immune responses. The aim of the present study was to determine the potential anti-inflammatory effects of PARP-1 inhibitor 5-aminoisoquinolinone (5-AIQ) to explore the role of PARP-1 inhibitor in a mouse model of carrageenan-induced lung inflammation. A single dose of 5-AIQ (1.5mg/kg) was administered intraperitoneally (i.p.) 1h before λ-carrageenan (Cg) administration. We assessed the effects of 5-AIQ treatment on CD25(+), GITR(+), CD25(+)GITR(+), IL-17(+) and Foxp3(+) cells which were investigated using flowcytometry in pleural exudates and heparinized blood. We also evaluated mRNA expressions of IL-6, TNF-α, IL-1β, IL-10, CD11a, l-selectin (CD62L), ICAM-1, MCP-1, iNOS and COX-2 in the lung tissue. We further examined the effects of 5-AIQ on the key mediators of inflammation, namely COX-2, STAT-3, NF-kB p65, PARP-1, IkB-α and IL-4 protein expression in the lung tissue using western blotting. The results illustrated that the numbers of T cell subsets, IL-17(+) cytokine levels were markedly increased and Foxp3(+) production decreased in the Cg group. Furthermore, Cg-induced up-regulation of adhesion molecules, pro-inflammatory mediators and chemokine expressions. Western blot analysis revealed an increased protein expressions of COX-2, STAT-3 NF-kB p65 and PARP-1 and decreased IkB-α and IL-4 in the Cg group. PARP-1 inhibitor via 5-AIQ treatment reverses the action significantly of all the previously mentioned effects. Moreover, histological examinations revealed anti-inflammatory effects of 5-AIQ, whereas Cg-group aggravated Cg-induced inflammation. Present findings demonstrate the potent anti-inflammatory action of the PARP-1 inhibitor in acute lung injury induced by carrageenan. Elsevier 2015-02-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/66953/1/The%20role%20of%20poly%28ADP-ribose%29%20polymerase-1%20inhibitor%20in%20carrageenan-induced%20lung%20inflammation%20in%20mice..pdf application/pdf en http://irep.iium.edu.my/66953/7/66953_Scopus%20-%20Document%20details.pdf Ahmada, Sheikh Fayaz and Zoheir, Khairy M.A. and Ansari, Mushtaq Ahmad and Korashya, Hesham M. and Bakheet, Saleh A. and Ashour, Abdelkader Elbadawy Abbas and Al-Shabanaha, Othman A. and Al-harbi, Mohammed M. and Attiaa, Sabry M. (2015) The role of poly(ADP-ribose) polymerase-1 inhibitor in carrageenan-induced lung inflammation in mice. Molecular Immunology, 63 (2). pp. 394-405. ISSN 0161-5890 https://www.journals.elsevier.com/molecular-immunology https://doi.org/10.1016/j.molimm.2014.09.009
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic RB Pathology
spellingShingle RB Pathology
Ahmada, Sheikh Fayaz
Zoheir, Khairy M.A.
Ansari, Mushtaq Ahmad
Korashya, Hesham M.
Bakheet, Saleh A.
Ashour, Abdelkader Elbadawy Abbas
Al-Shabanaha, Othman A.
Al-harbi, Mohammed M.
Attiaa, Sabry M.
The role of poly(ADP-ribose) polymerase-1 inhibitor in carrageenan-induced lung inflammation in mice.
description Increasing indication is unveiling a role for poly(ADP-ribose) polymerase (PARP)-1 in the regulation of inflammatory/immune responses. The aim of the present study was to determine the potential anti-inflammatory effects of PARP-1 inhibitor 5-aminoisoquinolinone (5-AIQ) to explore the role of PARP-1 inhibitor in a mouse model of carrageenan-induced lung inflammation. A single dose of 5-AIQ (1.5mg/kg) was administered intraperitoneally (i.p.) 1h before λ-carrageenan (Cg) administration. We assessed the effects of 5-AIQ treatment on CD25(+), GITR(+), CD25(+)GITR(+), IL-17(+) and Foxp3(+) cells which were investigated using flowcytometry in pleural exudates and heparinized blood. We also evaluated mRNA expressions of IL-6, TNF-α, IL-1β, IL-10, CD11a, l-selectin (CD62L), ICAM-1, MCP-1, iNOS and COX-2 in the lung tissue. We further examined the effects of 5-AIQ on the key mediators of inflammation, namely COX-2, STAT-3, NF-kB p65, PARP-1, IkB-α and IL-4 protein expression in the lung tissue using western blotting. The results illustrated that the numbers of T cell subsets, IL-17(+) cytokine levels were markedly increased and Foxp3(+) production decreased in the Cg group. Furthermore, Cg-induced up-regulation of adhesion molecules, pro-inflammatory mediators and chemokine expressions. Western blot analysis revealed an increased protein expressions of COX-2, STAT-3 NF-kB p65 and PARP-1 and decreased IkB-α and IL-4 in the Cg group. PARP-1 inhibitor via 5-AIQ treatment reverses the action significantly of all the previously mentioned effects. Moreover, histological examinations revealed anti-inflammatory effects of 5-AIQ, whereas Cg-group aggravated Cg-induced inflammation. Present findings demonstrate the potent anti-inflammatory action of the PARP-1 inhibitor in acute lung injury induced by carrageenan.
format Article
author Ahmada, Sheikh Fayaz
Zoheir, Khairy M.A.
Ansari, Mushtaq Ahmad
Korashya, Hesham M.
Bakheet, Saleh A.
Ashour, Abdelkader Elbadawy Abbas
Al-Shabanaha, Othman A.
Al-harbi, Mohammed M.
Attiaa, Sabry M.
author_facet Ahmada, Sheikh Fayaz
Zoheir, Khairy M.A.
Ansari, Mushtaq Ahmad
Korashya, Hesham M.
Bakheet, Saleh A.
Ashour, Abdelkader Elbadawy Abbas
Al-Shabanaha, Othman A.
Al-harbi, Mohammed M.
Attiaa, Sabry M.
author_sort Ahmada, Sheikh Fayaz
title The role of poly(ADP-ribose) polymerase-1 inhibitor in carrageenan-induced lung inflammation in mice.
title_short The role of poly(ADP-ribose) polymerase-1 inhibitor in carrageenan-induced lung inflammation in mice.
title_full The role of poly(ADP-ribose) polymerase-1 inhibitor in carrageenan-induced lung inflammation in mice.
title_fullStr The role of poly(ADP-ribose) polymerase-1 inhibitor in carrageenan-induced lung inflammation in mice.
title_full_unstemmed The role of poly(ADP-ribose) polymerase-1 inhibitor in carrageenan-induced lung inflammation in mice.
title_sort role of poly(adp-ribose) polymerase-1 inhibitor in carrageenan-induced lung inflammation in mice.
publisher Elsevier
publishDate 2015
url http://irep.iium.edu.my/66953/1/The%20role%20of%20poly%28ADP-ribose%29%20polymerase-1%20inhibitor%20in%20carrageenan-induced%20lung%20inflammation%20in%20mice..pdf
http://irep.iium.edu.my/66953/7/66953_Scopus%20-%20Document%20details.pdf
http://irep.iium.edu.my/66953/
https://www.journals.elsevier.com/molecular-immunology
https://doi.org/10.1016/j.molimm.2014.09.009
_version_ 1643618508490145792
score 13.211869

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