Staff View: Stem cells-endothelial differentiation in 3D in vitro model

Stem cells-endothelial differentiation in 3D in vitro model

lntroduction: Multipotent stem cells are characterized by its self renewal ability and potential to differentiate into many types of cell lineages. This study is focusing on angiogenesis, where the differentiation target is to turn the dental stem cells into endothelial-like cells. Thus, a 3D human...

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Main Authors:	Md Hashim, Siti Nurnasihah, Yusof, Muhammad Fuad Hilmi, Zahari, Wafa’, Chandra, Hamshawagini, Ahmad Amin Noordin, Khairul Bariah, Kannan, Thirumulu Ponnuraj, Sheikh Abdul Hamid, Suzina, Mokhtar@Makhtar, Khairani Idah, Ahmad, Azlina
Format:	Conference or Workshop Item
Language:	English
Published:	2018
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/66590/1/Abstract%20ICMHS2018-Siti%20Nurnasihah.pdf http://irep.iium.edu.my/66590/
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spelling	my.iium.irep.665902018-10-15T07:08:44Z http://irep.iium.edu.my/66590/ Stem cells-endothelial differentiation in 3D in vitro model Md Hashim, Siti Nurnasihah Yusof, Muhammad Fuad Hilmi Zahari, Wafa’ Chandra, Hamshawagini Ahmad Amin Noordin, Khairul Bariah Kannan, Thirumulu Ponnuraj Sheikh Abdul Hamid, Suzina Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina RK Dentistry lntroduction: Multipotent stem cells are characterized by its self renewal ability and potential to differentiate into many types of cell lineages. This study is focusing on angiogenesis, where the differentiation target is to turn the dental stem cells into endothelial-like cells. Thus, a 3D human amniotic membrane (HAM)-based in vitro model mimicking angiogeneic microenvironment is proposed to further understand the endothelial differentiation process. The 3D human amniotic membrane (HAM)-based scaffold is made up of stem cells from human exfoliated deciduous teeth (SHED) cultured on HAM with the addition of vascular endothelial growth factor (VEGF). Objectives: To determine the capability of SHED to undergo endothelial-like differentiation on a 3D HAM-based model. Methodology: SHED was cultured in a complete medium of alpha-minimum essential medium (a-MEM). De-epithelialised glycerol-preserved HAM was used as a scaffold while VEGF was added to induce angiogenesis. Cells were cultured in 3 groups, namely, SHED treated with VEGF (SV), SHED cultured on HAM (SA) and SHED cultured on HAM treated with VEGF (SAV). The endothelial differentiation was evaluated by scanning electron microscope (SEM), haematoxylin and eosin (H&E) and one-step RT-PCR Results: The result of SEM showed that SHED had successfully differentiated into endothelial-like cells. Through H&E staining, SHED was found forming a monolayer structure on the stromal side of HAM from day 1 until 14 but infiltrated into the structure at day 21. Meanwhile, gene expression analysis revealed that treated SHED was able to retain its stemness along with the expression of endothelial markers. Conclusion: Our 3D HAM-based in vitro model with the addition of VEGF was able to promote SHED-endothelial differentiation. 2018-09-26 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/66590/1/Abstract%20ICMHS2018-Siti%20Nurnasihah.pdf Md Hashim, Siti Nurnasihah and Yusof, Muhammad Fuad Hilmi and Zahari, Wafa’ and Chandra, Hamshawagini and Ahmad Amin Noordin, Khairul Bariah and Kannan, Thirumulu Ponnuraj and Sheikh Abdul Hamid, Suzina and Mokhtar@Makhtar, Khairani Idah and Ahmad, Azlina (2018) Stem cells-endothelial differentiation in 3D in vitro model. In: 2nd International Conference on Medical and Health Sciences and 23rd National Conference on Medical and Health Sciences, 26-27th September 2018, Kubang Kerian, Kelantan. (Unpublished)
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English
topic	RK Dentistry
spellingShingle	RK Dentistry Md Hashim, Siti Nurnasihah Yusof, Muhammad Fuad Hilmi Zahari, Wafa’ Chandra, Hamshawagini Ahmad Amin Noordin, Khairul Bariah Kannan, Thirumulu Ponnuraj Sheikh Abdul Hamid, Suzina Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina Stem cells-endothelial differentiation in 3D in vitro model
description	lntroduction: Multipotent stem cells are characterized by its self renewal ability and potential to differentiate into many types of cell lineages. This study is focusing on angiogenesis, where the differentiation target is to turn the dental stem cells into endothelial-like cells. Thus, a 3D human amniotic membrane (HAM)-based in vitro model mimicking angiogeneic microenvironment is proposed to further understand the endothelial differentiation process. The 3D human amniotic membrane (HAM)-based scaffold is made up of stem cells from human exfoliated deciduous teeth (SHED) cultured on HAM with the addition of vascular endothelial growth factor (VEGF). Objectives: To determine the capability of SHED to undergo endothelial-like differentiation on a 3D HAM-based model. Methodology: SHED was cultured in a complete medium of alpha-minimum essential medium (a-MEM). De-epithelialised glycerol-preserved HAM was used as a scaffold while VEGF was added to induce angiogenesis. Cells were cultured in 3 groups, namely, SHED treated with VEGF (SV), SHED cultured on HAM (SA) and SHED cultured on HAM treated with VEGF (SAV). The endothelial differentiation was evaluated by scanning electron microscope (SEM), haematoxylin and eosin (H&E) and one-step RT-PCR Results: The result of SEM showed that SHED had successfully differentiated into endothelial-like cells. Through H&E staining, SHED was found forming a monolayer structure on the stromal side of HAM from day 1 until 14 but infiltrated into the structure at day 21. Meanwhile, gene expression analysis revealed that treated SHED was able to retain its stemness along with the expression of endothelial markers. Conclusion: Our 3D HAM-based in vitro model with the addition of VEGF was able to promote SHED-endothelial differentiation.
format	Conference or Workshop Item
author	Md Hashim, Siti Nurnasihah Yusof, Muhammad Fuad Hilmi Zahari, Wafa’ Chandra, Hamshawagini Ahmad Amin Noordin, Khairul Bariah Kannan, Thirumulu Ponnuraj Sheikh Abdul Hamid, Suzina Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina
author_facet	Md Hashim, Siti Nurnasihah Yusof, Muhammad Fuad Hilmi Zahari, Wafa’ Chandra, Hamshawagini Ahmad Amin Noordin, Khairul Bariah Kannan, Thirumulu Ponnuraj Sheikh Abdul Hamid, Suzina Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina
author_sort	Md Hashim, Siti Nurnasihah
title	Stem cells-endothelial differentiation in 3D in vitro model
title_short	Stem cells-endothelial differentiation in 3D in vitro model
title_full	Stem cells-endothelial differentiation in 3D in vitro model
title_fullStr	Stem cells-endothelial differentiation in 3D in vitro model
title_full_unstemmed	Stem cells-endothelial differentiation in 3D in vitro model
title_sort	stem cells-endothelial differentiation in 3d in vitro model
publishDate	2018
url	http://irep.iium.edu.my/66590/1/Abstract%20ICMHS2018-Siti%20Nurnasihah.pdf http://irep.iium.edu.my/66590/
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score	13.211869

Stem cells-endothelial differentiation in 3D in vitro model

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