Cover Image

The expression of microrna-744 (MIR-744) in nasopharyngeal carcinoma: a preliminary study

The aim of this study was to investigate the level of miR-744 expression in nasopharyngeal carcinoma (NPC) tumour tissue and to provide initial clue on its potential as biomarkers for early detection of NPC in a preliminary analysis. Total miRNAs was extracted from NPC tissue as well as normal nasop...

Full description

Saved in:
Bibliographic Details
Main Authors: Othman, Noor Syarmila, Leman, Wan Ishlah, Abdullah, Kahairi, Muhammad, Siti Aeshah @ Naznin, Kaderi, Mohd Arifin
Format: Article
Language:English
Published: IIUM Press, International Islamic University Malaysia 2018
Subjects:
RC Internal medicine
Online Access:http://irep.iium.edu.my/64881/1/64881_The%20expression%20of%20microrna-744.pdf
http://irep.iium.edu.my/64881/
http://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/113
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.iium.irep.64881
record_format dspace
spelling my.iium.irep.648812022-04-07T01:36:28Z http://irep.iium.edu.my/64881/ The expression of microrna-744 (MIR-744) in nasopharyngeal carcinoma: a preliminary study Othman, Noor Syarmila Leman, Wan Ishlah Abdullah, Kahairi Muhammad, Siti Aeshah @ Naznin Kaderi, Mohd Arifin RC Internal medicine The aim of this study was to investigate the level of miR-744 expression in nasopharyngeal carcinoma (NPC) tumour tissue and to provide initial clue on its potential as biomarkers for early detection of NPC in a preliminary analysis. Total miRNAs was extracted from NPC tissue as well as normal nasopharynx tissue taken from Hospital Tengku Ampuan Afzan (HTAA), Kuantan and converted into cDNA. The level of miR-744 expression in the cDNA was quantified using quantitative reverse transcription polymserase chain reaction (RT-qPCR) technique. The expression level of SNORD48 was measured simultaneously for each sample, which served as endogenous control. The difference in the expression of miR-744 in NPC and normal nasopharynx tissue were analysed using relative quantification, 2-ΔΔCT. In this preliminary analysis, this study found that miR-744 was upregulated in NPC as compared to normal nasopharynx tissue by 2.5 fold changes, respectively suggesting it may involve in progression of tumour. However, the finding is not significant and may not accurately reflect the overall population, due to small sample size involved in the study. Findings from the current study suggest the potential of miR-744 to serve as useful diagnostic and prognostic biomarker in NPC. IIUM Press, International Islamic University Malaysia 2018-07-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/64881/1/64881_The%20expression%20of%20microrna-744.pdf Othman, Noor Syarmila and Leman, Wan Ishlah and Abdullah, Kahairi and Muhammad, Siti Aeshah @ Naznin and Kaderi, Mohd Arifin (2018) The expression of microrna-744 (MIR-744) in nasopharyngeal carcinoma: a preliminary study. International Journal of Allied Health Sciences, 2 (1). pp. 191-203. E-ISSN 2600-8491 http://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/113
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic RC Internal medicine
spellingShingle RC Internal medicine
Othman, Noor Syarmila
Leman, Wan Ishlah
Abdullah, Kahairi
Muhammad, Siti Aeshah @ Naznin
Kaderi, Mohd Arifin
The expression of microrna-744 (MIR-744) in nasopharyngeal carcinoma: a preliminary study
description The aim of this study was to investigate the level of miR-744 expression in nasopharyngeal carcinoma (NPC) tumour tissue and to provide initial clue on its potential as biomarkers for early detection of NPC in a preliminary analysis. Total miRNAs was extracted from NPC tissue as well as normal nasopharynx tissue taken from Hospital Tengku Ampuan Afzan (HTAA), Kuantan and converted into cDNA. The level of miR-744 expression in the cDNA was quantified using quantitative reverse transcription polymserase chain reaction (RT-qPCR) technique. The expression level of SNORD48 was measured simultaneously for each sample, which served as endogenous control. The difference in the expression of miR-744 in NPC and normal nasopharynx tissue were analysed using relative quantification, 2-ΔΔCT. In this preliminary analysis, this study found that miR-744 was upregulated in NPC as compared to normal nasopharynx tissue by 2.5 fold changes, respectively suggesting it may involve in progression of tumour. However, the finding is not significant and may not accurately reflect the overall population, due to small sample size involved in the study. Findings from the current study suggest the potential of miR-744 to serve as useful diagnostic and prognostic biomarker in NPC.
format Article
author Othman, Noor Syarmila
Leman, Wan Ishlah
Abdullah, Kahairi
Muhammad, Siti Aeshah @ Naznin
Kaderi, Mohd Arifin
author_facet Othman, Noor Syarmila
Leman, Wan Ishlah
Abdullah, Kahairi
Muhammad, Siti Aeshah @ Naznin
Kaderi, Mohd Arifin
author_sort Othman, Noor Syarmila
title The expression of microrna-744 (MIR-744) in nasopharyngeal carcinoma: a preliminary study
title_short The expression of microrna-744 (MIR-744) in nasopharyngeal carcinoma: a preliminary study
title_full The expression of microrna-744 (MIR-744) in nasopharyngeal carcinoma: a preliminary study
title_fullStr The expression of microrna-744 (MIR-744) in nasopharyngeal carcinoma: a preliminary study
title_full_unstemmed The expression of microrna-744 (MIR-744) in nasopharyngeal carcinoma: a preliminary study
title_sort expression of microrna-744 (mir-744) in nasopharyngeal carcinoma: a preliminary study
publisher IIUM Press, International Islamic University Malaysia
publishDate 2018
url http://irep.iium.edu.my/64881/1/64881_The%20expression%20of%20microrna-744.pdf
http://irep.iium.edu.my/64881/
http://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/113
_version_ 1729702900935950336
score 13.211869

Similar Items