Staff View: In vitro anticancer potentials of ethyl p-methoxycinnamate (EPMC) an active ingredient of cekur (Kaempferia galanga) on human oral cancer cell lines differing in p53 status.

In vitro anticancer potentials of ethyl p-methoxycinnamate (EPMC) an active ingredient of cekur (Kaempferia galanga) on human oral cancer cell lines differing in p53 status.

Oral Squamous Cell Carcinoma (OSCC) is one of malignant tumors with poor prognosis resulting in major morbidity and mortality. Loss of p53 tumor suppressor function is a common event in numerous types of human cancer including OSCC and the status of the p53 has been known to play a role in resistanc...

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Main Authors:	Ichwan, Solachuddin J. A., Omar, Muhammad Nor, Kasmuri, Abdul Razak
Format:	Monograph
Language:	English
Published:	2018
Subjects:	RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/64441/1/Report%20EDW%20B14-102-0987-ed.pdf http://irep.iium.edu.my/64441/
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spelling	my.iium.irep.644412020-06-25T07:30:52Z http://irep.iium.edu.my/64441/ In vitro anticancer potentials of ethyl p-methoxycinnamate (EPMC) an active ingredient of cekur (Kaempferia galanga) on human oral cancer cell lines differing in p53 status. Ichwan, Solachuddin J. A. Omar, Muhammad Nor Kasmuri, Abdul Razak RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works RS Pharmacy and materia medica Oral Squamous Cell Carcinoma (OSCC) is one of malignant tumors with poor prognosis resulting in major morbidity and mortality. Loss of p53 tumor suppressor function is a common event in numerous types of human cancer including OSCC and the status of the p53 has been known to play a role in resistance against chemotherapy. Herbal remedies are known to provide lead compounds in the past and play a significant role in future in the treatment of cancer due to their minimal side effects. Kaempferia galanga is one of the herbal plant that have many medicinal properties. The plant extract has also been reported to possess in vitro anti-cancer activities on various cancer cell lines. Ethyl-p-methoxycinnamate (EPMC), a major component of Kaempferia galanga has been attributed to its medicinal benefits. We report here for the first time the anticancer potential of EPMC on oral cancer cells derived from human oral cavity, HSC-3 and Ca922 lines. MTT assay results showed that EPMC markedly induced cytotoxicity on HSC-3 (IC50 0.075 mg/mL) and Ca922 (IC50 0.085 mg/mL) cell lines. The apoptotic activity induced by EPMC was detected in both cell lines by flow cytometry and confirmed with Caspase 3/7 assay. Moreover, quantitative RT-PCR analysis revealed that EPMC-induced apoptosis in both lines elevated the mRNA levels of pro-apoptotic genes caspase-9 and p53 target-PUMA. These results suggest that EPMC may be a potential candidate for oral cancer treatment. 2018-06-26 Monograph NonPeerReviewed application/pdf en http://irep.iium.edu.my/64441/1/Report%20EDW%20B14-102-0987-ed.pdf Ichwan, Solachuddin J. A. and Omar, Muhammad Nor and Kasmuri, Abdul Razak (2018) In vitro anticancer potentials of ethyl p-methoxycinnamate (EPMC) an active ingredient of cekur (Kaempferia galanga) on human oral cancer cell lines differing in p53 status. Project Report. UNSPECIFIED. (Unpublished)
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English
topic	RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works RS Pharmacy and materia medica
spellingShingle	RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works RS Pharmacy and materia medica Ichwan, Solachuddin J. A. Omar, Muhammad Nor Kasmuri, Abdul Razak In vitro anticancer potentials of ethyl p-methoxycinnamate (EPMC) an active ingredient of cekur (Kaempferia galanga) on human oral cancer cell lines differing in p53 status.
description	Oral Squamous Cell Carcinoma (OSCC) is one of malignant tumors with poor prognosis resulting in major morbidity and mortality. Loss of p53 tumor suppressor function is a common event in numerous types of human cancer including OSCC and the status of the p53 has been known to play a role in resistance against chemotherapy. Herbal remedies are known to provide lead compounds in the past and play a significant role in future in the treatment of cancer due to their minimal side effects. Kaempferia galanga is one of the herbal plant that have many medicinal properties. The plant extract has also been reported to possess in vitro anti-cancer activities on various cancer cell lines. Ethyl-p-methoxycinnamate (EPMC), a major component of Kaempferia galanga has been attributed to its medicinal benefits. We report here for the first time the anticancer potential of EPMC on oral cancer cells derived from human oral cavity, HSC-3 and Ca922 lines. MTT assay results showed that EPMC markedly induced cytotoxicity on HSC-3 (IC50 0.075 mg/mL) and Ca922 (IC50 0.085 mg/mL) cell lines. The apoptotic activity induced by EPMC was detected in both cell lines by flow cytometry and confirmed with Caspase 3/7 assay. Moreover, quantitative RT-PCR analysis revealed that EPMC-induced apoptosis in both lines elevated the mRNA levels of pro-apoptotic genes caspase-9 and p53 target-PUMA. These results suggest that EPMC may be a potential candidate for oral cancer treatment.
format	Monograph
author	Ichwan, Solachuddin J. A. Omar, Muhammad Nor Kasmuri, Abdul Razak
author_facet	Ichwan, Solachuddin J. A. Omar, Muhammad Nor Kasmuri, Abdul Razak
author_sort	Ichwan, Solachuddin J. A.
title	In vitro anticancer potentials of ethyl p-methoxycinnamate (EPMC) an active ingredient of cekur (Kaempferia galanga) on human oral cancer cell lines differing in p53 status.
title_short	In vitro anticancer potentials of ethyl p-methoxycinnamate (EPMC) an active ingredient of cekur (Kaempferia galanga) on human oral cancer cell lines differing in p53 status.
title_full	In vitro anticancer potentials of ethyl p-methoxycinnamate (EPMC) an active ingredient of cekur (Kaempferia galanga) on human oral cancer cell lines differing in p53 status.
title_fullStr	In vitro anticancer potentials of ethyl p-methoxycinnamate (EPMC) an active ingredient of cekur (Kaempferia galanga) on human oral cancer cell lines differing in p53 status.
title_full_unstemmed	In vitro anticancer potentials of ethyl p-methoxycinnamate (EPMC) an active ingredient of cekur (Kaempferia galanga) on human oral cancer cell lines differing in p53 status.
title_sort	in vitro anticancer potentials of ethyl p-methoxycinnamate (epmc) an active ingredient of cekur (kaempferia galanga) on human oral cancer cell lines differing in p53 status.
publishDate	2018
url	http://irep.iium.edu.my/64441/1/Report%20EDW%20B14-102-0987-ed.pdf http://irep.iium.edu.my/64441/
_version_	1672610117450727424
score	13.211869

In vitro anticancer potentials of ethyl p-methoxycinnamate (EPMC) an active ingredient of cekur (Kaempferia galanga) on human oral cancer cell lines differing in p53 status.

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