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Ultra-structural effects of different low-level lasers on normal cultured human melanocytes: An in vitro comparative study

The aim of this study was to investigate the effects of the different types of low-level laser therapy (LLLT) on the ultra-structure and number of melanosomes in normal cultured human melanocytes. Specific effects of various types of LLLT on the ultra-structure of melanosomes have not yet been repor...

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Main Authors:	AlGhamdi, Khalid M., Kumar, Ashok, Al-ghamdi, Attieh A, Al-Rikab, Ammar C., Mubarek, Mohammed, Ashour, Abdelkader Elbadawy Abbas
Format:	Article
Language:	English
Published:	Springer Nature 2016
Subjects:	RL Dermatology
Online Access:	http://irep.iium.edu.my/64276/7/64276%20Ultra-structural%20effects%20of%20different%20low-level%20lasers.pdf http://irep.iium.edu.my/64276/ https://link.springer.com/article/10.1007/s10103-016-2057-x
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spelling	my.iium.irep.64276 http://irep.iium.edu.my/64276/ Ultra-structural effects of different low-level lasers on normal cultured human melanocytes: An in vitro comparative study AlGhamdi, Khalid M. Kumar, Ashok Al-ghamdi, Attieh A Al-Rikab, Ammar C. Mubarek, Mohammed Ashour, Abdelkader Elbadawy Abbas RL Dermatology The aim of this study was to investigate the effects of the different types of low-level laser therapy (LLLT) on the ultra-structure and number of melanosomes in normal cultured human melanocytes. Specific effects of various types of LLLT on the ultra-structure of melanosomes have not yet been reported. Melanocytes were exposed to LLLT at an energy level of 2.0 J/cm2, using a blue (457 nm), red (635 nm), or ultraviolet (UV) (355 nm) laser. After 72 h of irradiation, the melanocytes were fixed in 2.5 % glutaraldehyde (pH 7.2) phosphate buffer for 8 h and analyzed by transmission electron microscopy. Four developmental stages (I to IV) of melanosomes were observed, and their numbers were counted manually. The percentage of stages I, II, III, and IV melanosomes was 12.8, 14.2, 22.6, and 50.3 %, respectively, in the control (sham light). However, the melanosome percentages were 41.2, 5.4, 8.2, and 24.2 % in stages I, II, III, and IV, respectively, in the blue laser-treated group; 58.4, 6.1, 9.3, and 26.2 % for stages I, II, III, and IV, respectively, in the red laser-treated group; and 31.3, 11.1, 16.5, and 41.1 % for stages I, II, III, and IV, respectively, in the UV laser-treated group. The present data show that the amount of stage I is significantly higher (P < 0.0001) in the LLLT-treated cells compared to the control, which indicates significant stimulation of melanogenesis. The red laser was more effective than the other lasers. Moreover, the effects of LLLT on the ultra-structure of melanosomes need to be studied in a larger number of subject groups Springer Nature 2016-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/64276/7/64276%20Ultra-structural%20effects%20of%20different%20low-level%20lasers.pdf AlGhamdi, Khalid M. and Kumar, Ashok and Al-ghamdi, Attieh A and Al-Rikab, Ammar C. and Mubarek, Mohammed and Ashour, Abdelkader Elbadawy Abbas (2016) Ultra-structural effects of different low-level lasers on normal cultured human melanocytes: An in vitro comparative study. Lasers in Medical Science, 31 (9). pp. 1819-1825. ISSN 0268-8921 https://link.springer.com/article/10.1007/s10103-016-2057-x 10.1007/s10103-016-2057-x
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English
topic	RL Dermatology
spellingShingle	RL Dermatology AlGhamdi, Khalid M. Kumar, Ashok Al-ghamdi, Attieh A Al-Rikab, Ammar C. Mubarek, Mohammed Ashour, Abdelkader Elbadawy Abbas Ultra-structural effects of different low-level lasers on normal cultured human melanocytes: An in vitro comparative study
description	The aim of this study was to investigate the effects of the different types of low-level laser therapy (LLLT) on the ultra-structure and number of melanosomes in normal cultured human melanocytes. Specific effects of various types of LLLT on the ultra-structure of melanosomes have not yet been reported. Melanocytes were exposed to LLLT at an energy level of 2.0 J/cm2, using a blue (457 nm), red (635 nm), or ultraviolet (UV) (355 nm) laser. After 72 h of irradiation, the melanocytes were fixed in 2.5 % glutaraldehyde (pH 7.2) phosphate buffer for 8 h and analyzed by transmission electron microscopy. Four developmental stages (I to IV) of melanosomes were observed, and their numbers were counted manually. The percentage of stages I, II, III, and IV melanosomes was 12.8, 14.2, 22.6, and 50.3 %, respectively, in the control (sham light). However, the melanosome percentages were 41.2, 5.4, 8.2, and 24.2 % in stages I, II, III, and IV, respectively, in the blue laser-treated group; 58.4, 6.1, 9.3, and 26.2 % for stages I, II, III, and IV, respectively, in the red laser-treated group; and 31.3, 11.1, 16.5, and 41.1 % for stages I, II, III, and IV, respectively, in the UV laser-treated group. The present data show that the amount of stage I is significantly higher (P < 0.0001) in the LLLT-treated cells compared to the control, which indicates significant stimulation of melanogenesis. The red laser was more effective than the other lasers. Moreover, the effects of LLLT on the ultra-structure of melanosomes need to be studied in a larger number of subject groups
format	Article
author	AlGhamdi, Khalid M. Kumar, Ashok Al-ghamdi, Attieh A Al-Rikab, Ammar C. Mubarek, Mohammed Ashour, Abdelkader Elbadawy Abbas
author_facet	AlGhamdi, Khalid M. Kumar, Ashok Al-ghamdi, Attieh A Al-Rikab, Ammar C. Mubarek, Mohammed Ashour, Abdelkader Elbadawy Abbas
author_sort	AlGhamdi, Khalid M.
title	Ultra-structural effects of different low-level lasers on normal cultured human melanocytes: An in vitro comparative study
title_short	Ultra-structural effects of different low-level lasers on normal cultured human melanocytes: An in vitro comparative study
title_full	Ultra-structural effects of different low-level lasers on normal cultured human melanocytes: An in vitro comparative study
title_fullStr	Ultra-structural effects of different low-level lasers on normal cultured human melanocytes: An in vitro comparative study
title_full_unstemmed	Ultra-structural effects of different low-level lasers on normal cultured human melanocytes: An in vitro comparative study
title_sort	ultra-structural effects of different low-level lasers on normal cultured human melanocytes: an in vitro comparative study
publisher	Springer Nature
publishDate	2016
url	http://irep.iium.edu.my/64276/7/64276%20Ultra-structural%20effects%20of%20different%20low-level%20lasers.pdf http://irep.iium.edu.my/64276/ https://link.springer.com/article/10.1007/s10103-016-2057-x
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score	13.211869

Ultra-structural effects of different low-level lasers on normal cultured human melanocytes: An in vitro comparative study

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