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A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPMEGCMS

Background: Volatile organic compounds (VOCs) emitted from exhaled breath from human bodies have been proven to be a useful source of information for early lung cancer diagnosis. To date, there are still arguable information on the production and origin of significant VOCs of cancer cells. Thus, t...

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Main Authors: Thriumani, Reena, Zakaria, Ammar, Hashim, Yumi Zuhanis Has-Yun, Jeffree, Amanina Iymia, Mohamed Helmy, Khaled, Kamarudin, Latifah Munirah, Omar, Mohammad Iqbal, Md Shakaff, Ali Yeon, Adom, Abdul Hamid, Persaud, Krishna C
Format: Article
Language:English
English
Published: BioMed Central Ltd. 2018
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/63611/1/Paper_Thriumani%20et%20al%202018%20%28BMC%20Cancer%29.pdf
http://irep.iium.edu.my/63611/7/63611-A%20study%20on%20volatile%20organic-SCOPUS.pdf
http://irep.iium.edu.my/63611/
https://bmccancer.biomedcentral.com/track/pdf/10.1186/s12885-018-4235-7
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spelling my.iium.irep.63611 http://irep.iium.edu.my/63611/ A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPMEGCMS Thriumani, Reena Zakaria, Ammar Hashim, Yumi Zuhanis Has-Yun Jeffree, Amanina Iymia Mohamed Helmy, Khaled Kamarudin, Latifah Munirah Omar, Mohammad Iqbal Md Shakaff, Ali Yeon Adom, Abdul Hamid Persaud, Krishna C Q Science (General) Background: Volatile organic compounds (VOCs) emitted from exhaled breath from human bodies have been proven to be a useful source of information for early lung cancer diagnosis. To date, there are still arguable information on the production and origin of significant VOCs of cancer cells. Thus, this study aims to conduct invitro experiments involving related cell lines to verify the capability of VOCs in providing information of the cells. Method: The performances of e-nose technology with different statistical methods to determine the best classifier were conducted and discussed. The gas sensor study has been complemented using solid phase micro-extractiongas chromatography mass spectrometry. For this purpose, the lung cancer cells (A549 and Calu-3) and control cell lines, breast cancer cell (MCF7) and non-cancerous lung cell (WI38VA13) were cultured in growth medium. Results: This study successfully provided a list of possible volatile organic compounds that can be specific biomarkers for lung cancer, even at the 24th hour of cell growth. Also, the Linear Discriminant Analysis-based One versus All-Support Vector Machine classifier, is able to produce high performance in distinguishing lung cancer from breast cancer cells and normal lung cells. Conclusion: The findings in this work conclude that the specific VOC released from the cancer cells can act as the odour signature and potentially to be used as non-invasive screening of lung cancer using gas array sensor devices. BioMed Central Ltd. 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/63611/1/Paper_Thriumani%20et%20al%202018%20%28BMC%20Cancer%29.pdf application/pdf en http://irep.iium.edu.my/63611/7/63611-A%20study%20on%20volatile%20organic-SCOPUS.pdf Thriumani, Reena and Zakaria, Ammar and Hashim, Yumi Zuhanis Has-Yun and Jeffree, Amanina Iymia and Mohamed Helmy, Khaled and Kamarudin, Latifah Munirah and Omar, Mohammad Iqbal and Md Shakaff, Ali Yeon and Adom, Abdul Hamid and Persaud, Krishna C (2018) A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPMEGCMS. BMC Cancer, 18 (1). pp. 1-17. ISSN 1471-2407 https://bmccancer.biomedcentral.com/track/pdf/10.1186/s12885-018-4235-7 10.1186/s12885-018-4235-7
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic Q Science (General)
spellingShingle Q Science (General)
Thriumani, Reena
Zakaria, Ammar
Hashim, Yumi Zuhanis Has-Yun
Jeffree, Amanina Iymia
Mohamed Helmy, Khaled
Kamarudin, Latifah Munirah
Omar, Mohammad Iqbal
Md Shakaff, Ali Yeon
Adom, Abdul Hamid
Persaud, Krishna C
A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPMEGCMS
description Background: Volatile organic compounds (VOCs) emitted from exhaled breath from human bodies have been proven to be a useful source of information for early lung cancer diagnosis. To date, there are still arguable information on the production and origin of significant VOCs of cancer cells. Thus, this study aims to conduct invitro experiments involving related cell lines to verify the capability of VOCs in providing information of the cells. Method: The performances of e-nose technology with different statistical methods to determine the best classifier were conducted and discussed. The gas sensor study has been complemented using solid phase micro-extractiongas chromatography mass spectrometry. For this purpose, the lung cancer cells (A549 and Calu-3) and control cell lines, breast cancer cell (MCF7) and non-cancerous lung cell (WI38VA13) were cultured in growth medium. Results: This study successfully provided a list of possible volatile organic compounds that can be specific biomarkers for lung cancer, even at the 24th hour of cell growth. Also, the Linear Discriminant Analysis-based One versus All-Support Vector Machine classifier, is able to produce high performance in distinguishing lung cancer from breast cancer cells and normal lung cells. Conclusion: The findings in this work conclude that the specific VOC released from the cancer cells can act as the odour signature and potentially to be used as non-invasive screening of lung cancer using gas array sensor devices.
format Article
author Thriumani, Reena
Zakaria, Ammar
Hashim, Yumi Zuhanis Has-Yun
Jeffree, Amanina Iymia
Mohamed Helmy, Khaled
Kamarudin, Latifah Munirah
Omar, Mohammad Iqbal
Md Shakaff, Ali Yeon
Adom, Abdul Hamid
Persaud, Krishna C
author_facet Thriumani, Reena
Zakaria, Ammar
Hashim, Yumi Zuhanis Has-Yun
Jeffree, Amanina Iymia
Mohamed Helmy, Khaled
Kamarudin, Latifah Munirah
Omar, Mohammad Iqbal
Md Shakaff, Ali Yeon
Adom, Abdul Hamid
Persaud, Krishna C
author_sort Thriumani, Reena
title A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPMEGCMS
title_short A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPMEGCMS
title_full A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPMEGCMS
title_fullStr A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPMEGCMS
title_full_unstemmed A study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and SPMEGCMS
title_sort study on volatile organic compounds emitted by in-vitro lung cancer cultured cells using gas sensor array and spmegcms
publisher BioMed Central Ltd.
publishDate 2018
url http://irep.iium.edu.my/63611/1/Paper_Thriumani%20et%20al%202018%20%28BMC%20Cancer%29.pdf
http://irep.iium.edu.my/63611/7/63611-A%20study%20on%20volatile%20organic-SCOPUS.pdf
http://irep.iium.edu.my/63611/
https://bmccancer.biomedcentral.com/track/pdf/10.1186/s12885-018-4235-7
_version_ 1643617141976465408
score 13.211869

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