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Modeling and development of screen-printed impedance biosensor for cytotoxicity studies of lung carcinoma cells

Electrical cell-substrate impedance sensing (ECIS) is a powerful technique to monitor real-time cell behavior. In this study, an ECIS biosensor formed using two interdigitated electrode structures (IDEs) was used to monitor cell behavior and its response to toxicants. Three different sensors with...

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Main Authors:	Mohd Mansor, Ahmad Fairuzabadi, Ibrahim, Irmanisha, Zainuddin, Ahmad Anwar, Voiculescu, Ioana, Nordin, Anis Nurashikin
Format:	Article
Language:	English English English English
Published:	Springer 2018
Subjects:	T Technology (General)
Online Access:	http://irep.iium.edu.my/63149/1/63149%20-Modeling%20and%20development%20of%20screen-printed%20impedance%20-Abstract-MyRA.pdf http://irep.iium.edu.my/63149/2/63149%20-Modeling%20and%20development%20of%20screen-printed%20impedance%20-SCOPUS.pdf http://irep.iium.edu.my/63149/13/63149_Modeling%20and%20development%20of%20screen-printed_WOS.pdf http://irep.iium.edu.my/63149/19/63149%20-Modeling%20and%20development%20of%20screen-printed%20impedance_article.pdf http://irep.iium.edu.my/63149/ https://link.springer.com/article/10.1007%2Fs11517-017-1756-1
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spelling	my.iium.irep.631492019-03-13T03:15:11Z http://irep.iium.edu.my/63149/ Modeling and development of screen-printed impedance biosensor for cytotoxicity studies of lung carcinoma cells Mohd Mansor, Ahmad Fairuzabadi Ibrahim, Irmanisha Zainuddin, Ahmad Anwar Voiculescu, Ioana Nordin, Anis Nurashikin T Technology (General) Electrical cell-substrate impedance sensing (ECIS) is a powerful technique to monitor real-time cell behavior. In this study, an ECIS biosensor formed using two interdigitated electrode structures (IDEs) was used to monitor cell behavior and its response to toxicants. Three different sensors with varied electrode spacing were first modeled using COMSOL Multiphysics and then fabricated and tested. The silver/silver chloride IDEs were fabricated using a screenprinting technique and incorporated with polydimethylsiloxane (PDMS) cell culture wells. To study the effectiveness of the biosensor, A549 lung carcinoma cells were seeded in the culture wells together with collagen as an extracellular matrix (ECM) to promote cell attachment on electrodes. A549 cells were cultured in the chambers and impedance measurements were taken at 12-h intervals for 120 h. Cell index (CI) for both designs were calculated from the impedance measurement and plotted in comparison with the growth profile of the cells in T-flasks. To verify that the ECIS biosensor can also be used to study cell response to toxicants, the A549 cells were also treated with anti-cancer drug, paclitaxel, and its responses were monitored over 5 days. Both simulation and experimental results show better sensitivity for smaller spacing between electrodes. Springer 2018-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/63149/1/63149%20-Modeling%20and%20development%20of%20screen-printed%20impedance%20-Abstract-MyRA.pdf application/pdf en http://irep.iium.edu.my/63149/2/63149%20-Modeling%20and%20development%20of%20screen-printed%20impedance%20-SCOPUS.pdf application/pdf en http://irep.iium.edu.my/63149/13/63149_Modeling%20and%20development%20of%20screen-printed_WOS.pdf application/pdf en http://irep.iium.edu.my/63149/19/63149%20-Modeling%20and%20development%20of%20screen-printed%20impedance_article.pdf Mohd Mansor, Ahmad Fairuzabadi and Ibrahim, Irmanisha and Zainuddin, Ahmad Anwar and Voiculescu, Ioana and Nordin, Anis Nurashikin (2018) Modeling and development of screen-printed impedance biosensor for cytotoxicity studies of lung carcinoma cells. Medical & Biological Engineering & Computing, 56 (1). pp. 173-181. ISSN 0140-0118 https://link.springer.com/article/10.1007%2Fs11517-017-1756-1 10.1007/s11517-017-1756-1
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English English English English
topic	T Technology (General)
spellingShingle	T Technology (General) Mohd Mansor, Ahmad Fairuzabadi Ibrahim, Irmanisha Zainuddin, Ahmad Anwar Voiculescu, Ioana Nordin, Anis Nurashikin Modeling and development of screen-printed impedance biosensor for cytotoxicity studies of lung carcinoma cells
description	Electrical cell-substrate impedance sensing (ECIS) is a powerful technique to monitor real-time cell behavior. In this study, an ECIS biosensor formed using two interdigitated electrode structures (IDEs) was used to monitor cell behavior and its response to toxicants. Three different sensors with varied electrode spacing were first modeled using COMSOL Multiphysics and then fabricated and tested. The silver/silver chloride IDEs were fabricated using a screenprinting technique and incorporated with polydimethylsiloxane (PDMS) cell culture wells. To study the effectiveness of the biosensor, A549 lung carcinoma cells were seeded in the culture wells together with collagen as an extracellular matrix (ECM) to promote cell attachment on electrodes. A549 cells were cultured in the chambers and impedance measurements were taken at 12-h intervals for 120 h. Cell index (CI) for both designs were calculated from the impedance measurement and plotted in comparison with the growth profile of the cells in T-flasks. To verify that the ECIS biosensor can also be used to study cell response to toxicants, the A549 cells were also treated with anti-cancer drug, paclitaxel, and its responses were monitored over 5 days. Both simulation and experimental results show better sensitivity for smaller spacing between electrodes.
format	Article
author	Mohd Mansor, Ahmad Fairuzabadi Ibrahim, Irmanisha Zainuddin, Ahmad Anwar Voiculescu, Ioana Nordin, Anis Nurashikin
author_facet	Mohd Mansor, Ahmad Fairuzabadi Ibrahim, Irmanisha Zainuddin, Ahmad Anwar Voiculescu, Ioana Nordin, Anis Nurashikin
author_sort	Mohd Mansor, Ahmad Fairuzabadi
title	Modeling and development of screen-printed impedance biosensor for cytotoxicity studies of lung carcinoma cells
title_short	Modeling and development of screen-printed impedance biosensor for cytotoxicity studies of lung carcinoma cells
title_full	Modeling and development of screen-printed impedance biosensor for cytotoxicity studies of lung carcinoma cells
title_fullStr	Modeling and development of screen-printed impedance biosensor for cytotoxicity studies of lung carcinoma cells
title_full_unstemmed	Modeling and development of screen-printed impedance biosensor for cytotoxicity studies of lung carcinoma cells
title_sort	modeling and development of screen-printed impedance biosensor for cytotoxicity studies of lung carcinoma cells
publisher	Springer
publishDate	2018
url	http://irep.iium.edu.my/63149/1/63149%20-Modeling%20and%20development%20of%20screen-printed%20impedance%20-Abstract-MyRA.pdf http://irep.iium.edu.my/63149/2/63149%20-Modeling%20and%20development%20of%20screen-printed%20impedance%20-SCOPUS.pdf http://irep.iium.edu.my/63149/13/63149_Modeling%20and%20development%20of%20screen-printed_WOS.pdf http://irep.iium.edu.my/63149/19/63149%20-Modeling%20and%20development%20of%20screen-printed%20impedance_article.pdf http://irep.iium.edu.my/63149/ https://link.springer.com/article/10.1007%2Fs11517-017-1756-1
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score	13.211869

Modeling and development of screen-printed impedance biosensor for cytotoxicity studies of lung carcinoma cells

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