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Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF)

Currently, virus separation process is mainly conducted by using ultracentrifugation or sucrose gradient electrophoresis methods. Both methods however have several drawbacks wherein the ultracentrifugation method requires very high rotational speed to separate the virus while the sucrose gradient el...

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Main Authors: Mel, Maizirwan, Arifin, Mohd Azmir, M Pauzi, Sazana
Format: Conference or Workshop Item
Language:English
Published: 2009
Subjects:
TP155 Chemical engineering
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/6299/1/Recovery_Virus_by_TFF.pdf
http://irep.iium.edu.my/6299/
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spelling my.iium.irep.62992011-11-21T19:54:06Z http://irep.iium.edu.my/6299/ Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF) Mel, Maizirwan Arifin, Mohd Azmir M Pauzi, Sazana TP155 Chemical engineering TP248.13 Biotechnology Currently, virus separation process is mainly conducted by using ultracentrifugation or sucrose gradient electrophoresis methods. Both methods however have several drawbacks wherein the ultracentrifugation method requires very high rotational speed to separate the virus while the sucrose gradient electrophoresis method is very time consuming. Alternative to both methods are by using Tangential Flow Filtration (TFF). In this study, we have separated and concentrated the Newcastle Disease Virus (NDV) harvested from embryonic specific pathogen free (SPF) eggs using TFF method by utilizing microfilter membrane with pore size of 0.45μm. Hollow fiber membrane was selected as filter because of their chemical and thermal stability. The study was conducted according to the design developed using Taguchi method which consists of selected three parameters and two levels of factors. The results revealed that higher pressure input (Pin), lower pressure output (Pout), and higher virus concentration led to higher virus titer. Optimum Trans-Membrane-Pressure (TMP) value of 15 psi and virus concentration of 28% had given the maximum titer of the virus which was 512 haemagglutination assay (HA) unit. 2009-08-14 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/6299/1/Recovery_Virus_by_TFF.pdf Mel, Maizirwan and Arifin, Mohd Azmir and M Pauzi, Sazana (2009) Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF). In: 3rd International Conference on Chemical & Bioprocess Engineering , 12–14 August 2009, Kota Kinabalu, Sabah.
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP155 Chemical engineering
TP248.13 Biotechnology
spellingShingle TP155 Chemical engineering
TP248.13 Biotechnology
Mel, Maizirwan
Arifin, Mohd Azmir
M Pauzi, Sazana
Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF)
description Currently, virus separation process is mainly conducted by using ultracentrifugation or sucrose gradient electrophoresis methods. Both methods however have several drawbacks wherein the ultracentrifugation method requires very high rotational speed to separate the virus while the sucrose gradient electrophoresis method is very time consuming. Alternative to both methods are by using Tangential Flow Filtration (TFF). In this study, we have separated and concentrated the Newcastle Disease Virus (NDV) harvested from embryonic specific pathogen free (SPF) eggs using TFF method by utilizing microfilter membrane with pore size of 0.45μm. Hollow fiber membrane was selected as filter because of their chemical and thermal stability. The study was conducted according to the design developed using Taguchi method which consists of selected three parameters and two levels of factors. The results revealed that higher pressure input (Pin), lower pressure output (Pout), and higher virus concentration led to higher virus titer. Optimum Trans-Membrane-Pressure (TMP) value of 15 psi and virus concentration of 28% had given the maximum titer of the virus which was 512 haemagglutination assay (HA) unit.
format Conference or Workshop Item
author Mel, Maizirwan
Arifin, Mohd Azmir
M Pauzi, Sazana
author_facet Mel, Maizirwan
Arifin, Mohd Azmir
M Pauzi, Sazana
author_sort Mel, Maizirwan
title Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF)
title_short Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF)
title_full Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF)
title_fullStr Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF)
title_full_unstemmed Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF)
title_sort recovery of virus producing ndv vaccine by tangential flow filtration (tff)
publishDate 2009
url http://irep.iium.edu.my/6299/1/Recovery_Virus_by_TFF.pdf
http://irep.iium.edu.my/6299/
_version_ 1643605705933979648
score 13.211869

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