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Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya

Introduction: Leaves of mempelas paya (Tetracera indica) (family: Dilleniaceae) is traditionally used in diabetes management in Malaysia. However, no attempt has ever been made to discover an active principle responsible for its blood glucose lowering effect. Hence, this research deals with the isol...

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Main Authors: Ahmed, Qamar Uddin, Mat So'ad, Siti Zaiton, Afrin, Mehnaz, Wan Sulaiman, Wan Mohd Azizi
Format: Conference or Workshop Item
Language:English
Published: Kulliyyah of Medicine, International Islamic University Malaysia 2016
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/61093/17/61093_Investigation%20of%20an%20antidiabetic%20potential_complete.pdf
http://irep.iium.edu.my/61093/
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spelling my.iium.irep.610932019-01-02T04:05:01Z http://irep.iium.edu.my/61093/ Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya Ahmed, Qamar Uddin Mat So'ad, Siti Zaiton Afrin, Mehnaz Wan Sulaiman, Wan Mohd Azizi QD Chemistry Introduction: Leaves of mempelas paya (Tetracera indica) (family: Dilleniaceae) is traditionally used in diabetes management in Malaysia. However, no attempt has ever been made to discover an active principle responsible for its blood glucose lowering effect. Hence, this research deals with the isolation, in vivo antidiabetic and toxicological evaluation of an active principle. Methods: Chemical investigation of methanol extract derived from the powdered leaves resulted in the isolation of a phenolic compound through repeated silica gel column chromatography. The structure was elucidated by spectroscopic (1H-NMR, 13C-NMR, MS, IR and UV) analysis. In vivo antidiabetic and toxicological studies were carried out using Sprague-Dawley rats (diabetic and normal) at three different concentrations (1, 5 and 25 mg/kg b.w.). Results: 5,7-dihydroxy-8-methoxyflavone was found to exhibit significant anti-hyperglycaemic activity (p<0.05) in diabetic rats at 5 and 25 mg/kg b.w. without showing hypoglycaemic effect in normal rats. The anti-hyperglycaemic activity was found to be comparable with glibenclamide (GLBC). In vivo toxicological study for continuous 21 days showed no abnormal activity and mortality. Histopathology of kidney, liver and pancreas of both normal and diabetic treated rats demonstrated normal and improved condition when compared to diabetic control group. There were no significant changes seen in the kidney (serum urea and creatinine) and lipid (TC, TG, LDL-C and HDL-C) functional parameters tested. Conclusions: 5,7-dihydroxy-8-methoxyflavone isolated from the leaves of mempelas paya coud be a safe antidiabetic agent. KEYWORDS: Tetracera indica, mempelas paya, 5,7-dihydroxy-8-methoxyflavone, in vivo, antidiabetic activity, toxicological study, histopathology Kulliyyah of Medicine, International Islamic University Malaysia 2016-08-04 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/61093/17/61093_Investigation%20of%20an%20antidiabetic%20potential_complete.pdf Ahmed, Qamar Uddin and Mat So'ad, Siti Zaiton and Afrin, Mehnaz and Wan Sulaiman, Wan Mohd Azizi (2016) Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya. In: Medical Research Symposium & Pacific Partnership in conjunction with Kuantan Research Day 2016, 4 August 2016, Kulliyyah of Medicine, Kuantan, Pahang. (Unpublished)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Ahmed, Qamar Uddin
Mat So'ad, Siti Zaiton
Afrin, Mehnaz
Wan Sulaiman, Wan Mohd Azizi
Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
description Introduction: Leaves of mempelas paya (Tetracera indica) (family: Dilleniaceae) is traditionally used in diabetes management in Malaysia. However, no attempt has ever been made to discover an active principle responsible for its blood glucose lowering effect. Hence, this research deals with the isolation, in vivo antidiabetic and toxicological evaluation of an active principle. Methods: Chemical investigation of methanol extract derived from the powdered leaves resulted in the isolation of a phenolic compound through repeated silica gel column chromatography. The structure was elucidated by spectroscopic (1H-NMR, 13C-NMR, MS, IR and UV) analysis. In vivo antidiabetic and toxicological studies were carried out using Sprague-Dawley rats (diabetic and normal) at three different concentrations (1, 5 and 25 mg/kg b.w.). Results: 5,7-dihydroxy-8-methoxyflavone was found to exhibit significant anti-hyperglycaemic activity (p<0.05) in diabetic rats at 5 and 25 mg/kg b.w. without showing hypoglycaemic effect in normal rats. The anti-hyperglycaemic activity was found to be comparable with glibenclamide (GLBC). In vivo toxicological study for continuous 21 days showed no abnormal activity and mortality. Histopathology of kidney, liver and pancreas of both normal and diabetic treated rats demonstrated normal and improved condition when compared to diabetic control group. There were no significant changes seen in the kidney (serum urea and creatinine) and lipid (TC, TG, LDL-C and HDL-C) functional parameters tested. Conclusions: 5,7-dihydroxy-8-methoxyflavone isolated from the leaves of mempelas paya coud be a safe antidiabetic agent. KEYWORDS: Tetracera indica, mempelas paya, 5,7-dihydroxy-8-methoxyflavone, in vivo, antidiabetic activity, toxicological study, histopathology
format Conference or Workshop Item
author Ahmed, Qamar Uddin
Mat So'ad, Siti Zaiton
Afrin, Mehnaz
Wan Sulaiman, Wan Mohd Azizi
author_facet Ahmed, Qamar Uddin
Mat So'ad, Siti Zaiton
Afrin, Mehnaz
Wan Sulaiman, Wan Mohd Azizi
author_sort Ahmed, Qamar Uddin
title Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
title_short Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
title_full Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
title_fullStr Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
title_full_unstemmed Investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
title_sort investigation of an antidiabetic potential of a phenolic compound isolated from the leaves of mempelas paya
publisher Kulliyyah of Medicine, International Islamic University Malaysia
publishDate 2016
url http://irep.iium.edu.my/61093/17/61093_Investigation%20of%20an%20antidiabetic%20potential_complete.pdf
http://irep.iium.edu.my/61093/
_version_ 1643618428537274368
score 13.211869

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