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Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica

The leaves of Tetracera indica are used in traditional medicine for the treatment of diabetes mellitus. This study was aimed at investigating the phytochemical constituents present in T. indica leaves and their activity against digestive enzyme (alphaglucosidase) which may be part of the mechanism o...

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Main Authors: Muhammad Alhassan, Alhassan, Ahmed, Qamar Uddin, Sarian, Murni Nazira, Khatib, Alfi
Format: Conference or Workshop Item
Language:English
English
Published: University Putra Malaysia 2017
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/61007/1/Alhassan_IABS%202017_0061.pdf
http://irep.iium.edu.my/61007/2/IABS%202017%20PROCEEDINGS.pdf
http://irep.iium.edu.my/61007/
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spelling my.iium.irep.610072017-12-31T15:16:13Z http://irep.iium.edu.my/61007/ Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica Muhammad Alhassan, Alhassan Ahmed, Qamar Uddin Sarian, Murni Nazira Khatib, Alfi QD Chemistry The leaves of Tetracera indica are used in traditional medicine for the treatment of diabetes mellitus. This study was aimed at investigating the phytochemical constituents present in T. indica leaves and their activity against digestive enzyme (alphaglucosidase) which may be part of the mechanism of the antidiabetic effect of the plant. Crude methanolic extract from the leaves of T. indica which demonstrate significant alpha glucosidase inhibitory activity was separated and purified by column chromatography of Silica gel, ODS and Sephadex LH-20, the structures of the isolated compounds were identified by UV, 1H-NMR, 13C-NMR and MS spectroscopic techniques. Five flavonoids were isolated from the leaves of T. indica, and these compounds were identified as follows: 5,7-dihydroxy-8-methoxyflavone (wogonin), 5,7,8-trihydroxyflavone (norwogonin), quercetin, 5- hydroxy-7-methoxyflavone (tectochrysin) and 41, 3, 5, 7-tetrahydroxyflavone (kaempferol). The isolated compounds show moderate to strong activity against alphaglucosidase enzymes. Insilco studies using molecular docking show the mode of interactions of the compounds with the active site residues and their binding energies. The results of this study reveal that alphaglucosidase inhibition is one of the primary mode of action of T.indica antidiabetic effect. University Putra Malaysia 2017-08-01 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/61007/1/Alhassan_IABS%202017_0061.pdf application/pdf en http://irep.iium.edu.my/61007/2/IABS%202017%20PROCEEDINGS.pdf Muhammad Alhassan, Alhassan and Ahmed, Qamar Uddin and Sarian, Murni Nazira and Khatib, Alfi (2017) Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica. In: 2nd International Anatomical and Biomedical Scientific Conference 2017, 1-2 Aug 2017, Serdang, Selangor.
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic QD Chemistry
spellingShingle QD Chemistry
Muhammad Alhassan, Alhassan
Ahmed, Qamar Uddin
Sarian, Murni Nazira
Khatib, Alfi
Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica
description The leaves of Tetracera indica are used in traditional medicine for the treatment of diabetes mellitus. This study was aimed at investigating the phytochemical constituents present in T. indica leaves and their activity against digestive enzyme (alphaglucosidase) which may be part of the mechanism of the antidiabetic effect of the plant. Crude methanolic extract from the leaves of T. indica which demonstrate significant alpha glucosidase inhibitory activity was separated and purified by column chromatography of Silica gel, ODS and Sephadex LH-20, the structures of the isolated compounds were identified by UV, 1H-NMR, 13C-NMR and MS spectroscopic techniques. Five flavonoids were isolated from the leaves of T. indica, and these compounds were identified as follows: 5,7-dihydroxy-8-methoxyflavone (wogonin), 5,7,8-trihydroxyflavone (norwogonin), quercetin, 5- hydroxy-7-methoxyflavone (tectochrysin) and 41, 3, 5, 7-tetrahydroxyflavone (kaempferol). The isolated compounds show moderate to strong activity against alphaglucosidase enzymes. Insilco studies using molecular docking show the mode of interactions of the compounds with the active site residues and their binding energies. The results of this study reveal that alphaglucosidase inhibition is one of the primary mode of action of T.indica antidiabetic effect.
format Conference or Workshop Item
author Muhammad Alhassan, Alhassan
Ahmed, Qamar Uddin
Sarian, Murni Nazira
Khatib, Alfi
author_facet Muhammad Alhassan, Alhassan
Ahmed, Qamar Uddin
Sarian, Murni Nazira
Khatib, Alfi
author_sort Muhammad Alhassan, Alhassan
title Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica
title_short Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica
title_full Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica
title_fullStr Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica
title_full_unstemmed Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica
title_sort insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica
publisher University Putra Malaysia
publishDate 2017
url http://irep.iium.edu.my/61007/1/Alhassan_IABS%202017_0061.pdf
http://irep.iium.edu.my/61007/2/IABS%202017%20PROCEEDINGS.pdf
http://irep.iium.edu.my/61007/
_version_ 1643615912638545920
score 13.211869

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