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In vitro antioxidant activities and gas-chromatography-mass spectrometry (GC-MS) analysis of antioxidant active fractions of Dialium indum var. indum L. fruit

The fruit of Dialium indum var. indum L. or locally known as ‘keranji’ from the family Fabaceae is one of the wild fruits of Malaysia. Measurement of in vitro antioxidant capacity and activity of various crude extracts and fractions of the fruit parts using total phenolic content (TPC), cupric ion r...

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Main Authors: Osman, Muhammad Faris, Mohd Hassan, Norazian, Khatib, Alfi
Format: Conference or Workshop Item
Language:English
Published: 2017
Subjects:
Q Science (General)
RS403 Materia Medica-Pharmaceutical Chemistry
Online Access:http://irep.iium.edu.my/60911/1/IMFSC2017-2.pdf
http://irep.iium.edu.my/60911/
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spelling my.iium.irep.609112018-01-09T07:15:53Z http://irep.iium.edu.my/60911/ In vitro antioxidant activities and gas-chromatography-mass spectrometry (GC-MS) analysis of antioxidant active fractions of Dialium indum var. indum L. fruit Osman, Muhammad Faris Mohd Hassan, Norazian Khatib, Alfi Q Science (General) RS403 Materia Medica-Pharmaceutical Chemistry The fruit of Dialium indum var. indum L. or locally known as ‘keranji’ from the family Fabaceae is one of the wild fruits of Malaysia. Measurement of in vitro antioxidant capacity and activity of various crude extracts and fractions of the fruit parts using total phenolic content (TPC), cupric ion reducing antioxidant capacity (CUPRAC), DPPH radical scavenging, and linoleic acid peroxidation inhibition assays revealed that exocarp dichloromethane fraction (EDF) and seed methanol fraction (SMF) were the most antioxidant active fractions. GC-MS analysis of trimethylsilyl (TMS)-derivatized EDF and SMF further revealed different area percentage of saccharides, fatty acids, amino acids, sesquiterpenes, polyols, dicarboxylic acids, and phenolics in their total ion current (TIC) chromatograms. Presence of eight known antioxidant phenolics namely vanillic acid, syringic acid, ferulic acid, isoferulic acid, vanillin, syringic aldehyde, p-hydroxybenzaldehyde, and coniferyl aldehyde were detected only in EDF while six antioxidant amino acids namely pyroglutamic acid, glutamic acid, phenylalanine, proline, serine, and threonine were detected only in SMF. Using GC-MS analysis, this study showed the distinctive classes of phytochemical constituents in the exocarp and seed of D. indum var. indum L. fruit, which collectively contributed to their good in vitro antioxidant capacities and activities. 2017 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/60911/1/IMFSC2017-2.pdf Osman, Muhammad Faris and Mohd Hassan, Norazian and Khatib, Alfi (2017) In vitro antioxidant activities and gas-chromatography-mass spectrometry (GC-MS) analysis of antioxidant active fractions of Dialium indum var. indum L. fruit. In: International Malaysian-French Scientific Conference (IMFSC 2017): Inspired by nature, delivered through science for a better world, 18-19 December 2017, Kuala Lumpur, Malaysia. (Unpublished)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic Q Science (General)
RS403 Materia Medica-Pharmaceutical Chemistry
spellingShingle Q Science (General)
RS403 Materia Medica-Pharmaceutical Chemistry
Osman, Muhammad Faris
Mohd Hassan, Norazian
Khatib, Alfi
In vitro antioxidant activities and gas-chromatography-mass spectrometry (GC-MS) analysis of antioxidant active fractions of Dialium indum var. indum L. fruit
description The fruit of Dialium indum var. indum L. or locally known as ‘keranji’ from the family Fabaceae is one of the wild fruits of Malaysia. Measurement of in vitro antioxidant capacity and activity of various crude extracts and fractions of the fruit parts using total phenolic content (TPC), cupric ion reducing antioxidant capacity (CUPRAC), DPPH radical scavenging, and linoleic acid peroxidation inhibition assays revealed that exocarp dichloromethane fraction (EDF) and seed methanol fraction (SMF) were the most antioxidant active fractions. GC-MS analysis of trimethylsilyl (TMS)-derivatized EDF and SMF further revealed different area percentage of saccharides, fatty acids, amino acids, sesquiterpenes, polyols, dicarboxylic acids, and phenolics in their total ion current (TIC) chromatograms. Presence of eight known antioxidant phenolics namely vanillic acid, syringic acid, ferulic acid, isoferulic acid, vanillin, syringic aldehyde, p-hydroxybenzaldehyde, and coniferyl aldehyde were detected only in EDF while six antioxidant amino acids namely pyroglutamic acid, glutamic acid, phenylalanine, proline, serine, and threonine were detected only in SMF. Using GC-MS analysis, this study showed the distinctive classes of phytochemical constituents in the exocarp and seed of D. indum var. indum L. fruit, which collectively contributed to their good in vitro antioxidant capacities and activities.
format Conference or Workshop Item
author Osman, Muhammad Faris
Mohd Hassan, Norazian
Khatib, Alfi
author_facet Osman, Muhammad Faris
Mohd Hassan, Norazian
Khatib, Alfi
author_sort Osman, Muhammad Faris
title In vitro antioxidant activities and gas-chromatography-mass spectrometry (GC-MS) analysis of antioxidant active fractions of Dialium indum var. indum L. fruit
title_short In vitro antioxidant activities and gas-chromatography-mass spectrometry (GC-MS) analysis of antioxidant active fractions of Dialium indum var. indum L. fruit
title_full In vitro antioxidant activities and gas-chromatography-mass spectrometry (GC-MS) analysis of antioxidant active fractions of Dialium indum var. indum L. fruit
title_fullStr In vitro antioxidant activities and gas-chromatography-mass spectrometry (GC-MS) analysis of antioxidant active fractions of Dialium indum var. indum L. fruit
title_full_unstemmed In vitro antioxidant activities and gas-chromatography-mass spectrometry (GC-MS) analysis of antioxidant active fractions of Dialium indum var. indum L. fruit
title_sort in vitro antioxidant activities and gas-chromatography-mass spectrometry (gc-ms) analysis of antioxidant active fractions of dialium indum var. indum l. fruit
publishDate 2017
url http://irep.iium.edu.my/60911/1/IMFSC2017-2.pdf
http://irep.iium.edu.my/60911/
_version_ 1643615895737597952
score 13.211869

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