Antioxidant activity and phenolic profile of four Saudi Arabia date palms (Phoenix dactylifera L.) varieties
Antioxidants as free radical scavengers can combat the oxidative stress that occurs in the body to prevent chronic diseases. Therefore, antioxidant rich foods have a high demand among the healthcare consumers to maintain healthy lifestyle. One of the most potential fruit that are expected to contain...
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my.iium.irep.605532018-05-05T17:11:14Z http://irep.iium.edu.my/60553/ Antioxidant activity and phenolic profile of four Saudi Arabia date palms (Phoenix dactylifera L.) varieties Hamzah, Nurfatin Nazirah Ferdosh, Sahena Sarker, Md. Zaidul Islam Ghafoor, Kashif RS192 Materia Medica-Pharmaceutical Technology Antioxidants as free radical scavengers can combat the oxidative stress that occurs in the body to prevent chronic diseases. Therefore, antioxidant rich foods have a high demand among the healthcare consumers to maintain healthy lifestyle. One of the most potential fruit that are expected to contain high antioxidant compound is Phoenix dactylifera that have a lot of varieties. This study was intended to investigate the total phenolic content, antioxidant activity and phenolic profiles of four varieties of P. dactylifera (Khudori, Sabaka, Nabtat Ali and Soukari). The fleshes of the fruit were extracted using ethanol as the solvent using soxhlet. Total phenolic content (TPC) was measured by using Folin-Ciocalteu calorimetric method and the antioxidant activity was determined by using the 2,2,diphenyl-2-picryl-hydrazyl (DPPH) method. Some phenolic acid and flavonoids profiles of the fruit (gallic acid, p-coumaric acid, ferulic acid and quercetin) were analyzed by using High Performance Liquid Chromatography (HPLC). TPC of the fruit was in the range of 5.8 to 3.8 mg gallic acid equivalents (GAE) per 1 g of fruit with Soukari have the highest value. As for the antioxidant activity, Soukari need the lowest concentration (27.41 ± 1.68 mg/ml) to achieve half inhibition concentration (IC50 ) whereas Khudori need the highest concentration among the four. The HPLC result shows significantly higher amount of gallic acid (15.35 to 8.56 mg/g), and very small amount of ferulic acid (0.19 to 0.006 mg/g) where Soukari have the highest value for all of the compounds. This study demonstrates Soukari variety might possessed the highest antioxidant capacities among the four. Further study by using advanced and green extraction method need to be carried out to comprehend and confirm the potential of this fruit to be developed as nutraceutical and functional food. Keywords: Pheonix dactylifera, Antioxidant, Phenolic profile, HPLC. 2017-08-08 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/60553/1/ICAST2017_O-11.pdf application/pdf en http://irep.iium.edu.my/60553/7/60553_Conference%20Program.pdf Hamzah, Nurfatin Nazirah and Ferdosh, Sahena and Sarker, Md. Zaidul Islam and Ghafoor, Kashif (2017) Antioxidant activity and phenolic profile of four Saudi Arabia date palms (Phoenix dactylifera L.) varieties. In: the 6th International Conference on Advancement in Science and Technology (iCAST 2017)., 8-9 Aug 2017, Kulliyyah of Science, International Islamic University Malaysia, Kuantan Campus.Pahang, Malaysia. (Unpublished) 11 |
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RS192 Materia Medica-Pharmaceutical Technology Hamzah, Nurfatin Nazirah Ferdosh, Sahena Sarker, Md. Zaidul Islam Ghafoor, Kashif Antioxidant activity and phenolic profile of four Saudi Arabia date palms (Phoenix dactylifera L.) varieties |
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Antioxidants as free radical scavengers can combat the oxidative stress that occurs in the body to prevent chronic diseases. Therefore, antioxidant rich foods have a high demand among the healthcare consumers to maintain healthy lifestyle. One of the most potential fruit that are expected to contain high antioxidant compound is Phoenix dactylifera that have a lot of varieties. This study was intended to investigate the total phenolic content, antioxidant activity and phenolic profiles of four varieties of P. dactylifera (Khudori, Sabaka, Nabtat Ali and Soukari). The fleshes of the fruit were extracted using ethanol as the solvent using soxhlet. Total phenolic content (TPC) was measured by using Folin-Ciocalteu calorimetric method and the antioxidant
activity was determined by using the 2,2,diphenyl-2-picryl-hydrazyl (DPPH) method. Some phenolic acid and flavonoids profiles of the fruit (gallic acid, p-coumaric acid, ferulic acid and quercetin) were analyzed by using High Performance Liquid Chromatography (HPLC). TPC of
the fruit was in the range of 5.8 to 3.8 mg gallic acid equivalents (GAE) per 1 g of fruit with Soukari have the highest value. As for the antioxidant activity, Soukari need the lowest concentration (27.41 ± 1.68 mg/ml) to achieve half inhibition concentration (IC50 ) whereas
Khudori need the highest concentration among the four. The HPLC result shows significantly higher amount of gallic acid (15.35 to 8.56 mg/g), and very small amount of ferulic acid (0.19 to 0.006 mg/g) where Soukari have the highest value for all of the compounds. This study
demonstrates Soukari variety might possessed the highest antioxidant capacities among the four. Further study by using advanced and green extraction method need to be carried out to comprehend and confirm the potential of this fruit to be developed as nutraceutical and
functional food. Keywords: Pheonix dactylifera, Antioxidant, Phenolic profile, HPLC. |
format |
Conference or Workshop Item |
author |
Hamzah, Nurfatin Nazirah Ferdosh, Sahena Sarker, Md. Zaidul Islam Ghafoor, Kashif |
author_facet |
Hamzah, Nurfatin Nazirah Ferdosh, Sahena Sarker, Md. Zaidul Islam Ghafoor, Kashif |
author_sort |
Hamzah, Nurfatin Nazirah |
title |
Antioxidant activity and phenolic profile of four Saudi Arabia date palms (Phoenix dactylifera L.) varieties |
title_short |
Antioxidant activity and phenolic profile of four Saudi Arabia date palms (Phoenix dactylifera L.) varieties |
title_full |
Antioxidant activity and phenolic profile of four Saudi Arabia date palms (Phoenix dactylifera L.) varieties |
title_fullStr |
Antioxidant activity and phenolic profile of four Saudi Arabia date palms (Phoenix dactylifera L.) varieties |
title_full_unstemmed |
Antioxidant activity and phenolic profile of four Saudi Arabia date palms (Phoenix dactylifera L.) varieties |
title_sort |
antioxidant activity and phenolic profile of four saudi arabia date palms (phoenix dactylifera l.) varieties |
publishDate |
2017 |
url |
http://irep.iium.edu.my/60553/1/ICAST2017_O-11.pdf http://irep.iium.edu.my/60553/7/60553_Conference%20Program.pdf http://irep.iium.edu.my/60553/ |
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