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Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor

The purpose of this study was to optimize agitation speed, microcarrier concentration and pO2 levels in bioreactor for culturing DF-1cells in DMEM media supplemented with 7% fetal bovine serum. Statistical analysis has shown that the model derived from the results was proven to be significant and pO...

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Main Authors: Mel, Maizirwan, Arifin, Mohd Azmir, Sohif, Hajar Naemah, Syed Hasan, Sharifah
Format: Article
Language:English
Published: Malaysian Medical Associations 2010
Subjects:
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/5943/1/Paper_MJM_Azmir.pdf
http://irep.iium.edu.my/5943/
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spelling my.iium.irep.59432011-11-29T07:31:35Z http://irep.iium.edu.my/5943/ Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor Mel, Maizirwan Arifin, Mohd Azmir Sohif, Hajar Naemah Syed Hasan, Sharifah TP248.13 Biotechnology The purpose of this study was to optimize agitation speed, microcarrier concentration and pO2 levels in bioreactor for culturing DF-1cells in DMEM media supplemented with 7% fetal bovine serum. Statistical analysis has shown that the model derived from the results was proven to be significant and pO2 level turned out as the most significant parameter in the experiment. As predicted by analysis, cells grew up to 1.190 x 106 cells/ml when moderate agitation speed, microcarrier concentration, and pO2 level are applied. Malaysian Medical Associations 2010 Article REM application/pdf en http://irep.iium.edu.my/5943/1/Paper_MJM_Azmir.pdf Mel, Maizirwan and Arifin, Mohd Azmir and Sohif, Hajar Naemah and Syed Hasan, Sharifah (2010) Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor. Medical Journal of Malaysia, 65 . pp. 19-21. ISSN 0300-5283
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Mel, Maizirwan
Arifin, Mohd Azmir
Sohif, Hajar Naemah
Syed Hasan, Sharifah
Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
description The purpose of this study was to optimize agitation speed, microcarrier concentration and pO2 levels in bioreactor for culturing DF-1cells in DMEM media supplemented with 7% fetal bovine serum. Statistical analysis has shown that the model derived from the results was proven to be significant and pO2 level turned out as the most significant parameter in the experiment. As predicted by analysis, cells grew up to 1.190 x 106 cells/ml when moderate agitation speed, microcarrier concentration, and pO2 level are applied.
format Article
author Mel, Maizirwan
Arifin, Mohd Azmir
Sohif, Hajar Naemah
Syed Hasan, Sharifah
author_facet Mel, Maizirwan
Arifin, Mohd Azmir
Sohif, Hajar Naemah
Syed Hasan, Sharifah
author_sort Mel, Maizirwan
title Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
title_short Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
title_full Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
title_fullStr Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
title_full_unstemmed Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
title_sort optimization of process conditions for high cell density proliferation of df-1 cells in bioreactor
publisher Malaysian Medical Associations
publishDate 2010
url http://irep.iium.edu.my/5943/1/Paper_MJM_Azmir.pdf
http://irep.iium.edu.my/5943/
_version_ 1643605645660782592
score 13.211869

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