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Autologous serum supplement favours in vitro regenerative paracrine factors synthesis

Objectives: Foetal bovine serum (FBS) is often the serum supplement of choice for in vitro human cell culture. This study compares the effect of FBS and autologous human serum (AuHS) supplement in human peripheral blood mononuclear cell (PBMC) culture to prepare secretome. Materials and methods: The...

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Main Authors: Haque, Nazmul, Abu Kasim, Noor Hayaty, Abu Kassim, Noor Lide, Rahman, Mohammad Tariqur
Format: Article
Language:English
English
English
Published: Wiley-Blackwell 2017
Subjects:
RC Internal medicine
RE Ophthalmology
RK Dentistry
Online Access:http://irep.iium.edu.my/59216/1/59216_Autologous%20serum%20supplement%20favours_article.pdf
http://irep.iium.edu.my/59216/2/59216_Autologous%20serum%20supplement%20favours_scopus.pdf
http://irep.iium.edu.my/59216/13/59216_Autologous%20serum%20supplement%20favours_ABSTRACT.pdf
http://irep.iium.edu.my/59216/
http://onlinelibrary.wiley.com/doi/10.1111/cpr.12354/abstract
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spelling my.iium.irep.59216 http://irep.iium.edu.my/59216/ Autologous serum supplement favours in vitro regenerative paracrine factors synthesis Haque, Nazmul Abu Kasim, Noor Hayaty Abu Kassim, Noor Lide Rahman, Mohammad Tariqur RC Internal medicine RE Ophthalmology RK Dentistry Objectives: Foetal bovine serum (FBS) is often the serum supplement of choice for in vitro human cell culture. This study compares the effect of FBS and autologous human serum (AuHS) supplement in human peripheral blood mononuclear cell (PBMC) culture to prepare secretome. Materials and methods: The PBMC (n = 7) were cultured either in RPMI-1640 containing L-glutamine and 50 units/ml Penicillin-Streptomycin (BM) or in BM with either AuHS or FBS. Viability, proliferation and differentiation of PBMC were evaluated. Paracrine factors present in the secretomes (n = 6) were analysed using ProcartaPlex Human Cytokine panel (17 plex). Ingenuity Pathway Analysis (IPA) was performed to predict activation or inhibition of biological functions related to tissue regeneration. Results: The viability of PBMC that were cultured with FBS supplement was significantly reduced at 96 h compared to those at 0 and 24 h (P <.05). While the reduction of the viability of PBMC that were cultured with AuHS supplement was not significantly different compared to those at 0 and 24 h. The FBS secretomes prepared at 24 h was found to contain significantly higher amount of EGF (P <.05) compared to that in AuHS or BM secretome. The AuHS secretomes contained significantly higher amount of HGF at 24 (P <.05) and 96 h (P <.01), and VEGF-A at 24 h (P <.05) compared to those in the FBS secretomes. SDF-1 was not detected in the FBS secretomes prepared at either 24 or 96 hours. Double immunocytochemical staining revealed a marked increase in co-localization of SDF-1 and its receptor in PBMC that were cultured with AuHS supplement compared to that cultured with FBS supplement. Conclusion: In secretome preparation, AuHS supplement favours synthesis of paracrine factors that are needed for regenerative therapy. Wiley-Blackwell 2017-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/59216/1/59216_Autologous%20serum%20supplement%20favours_article.pdf application/pdf en http://irep.iium.edu.my/59216/2/59216_Autologous%20serum%20supplement%20favours_scopus.pdf application/pdf en http://irep.iium.edu.my/59216/13/59216_Autologous%20serum%20supplement%20favours_ABSTRACT.pdf Haque, Nazmul and Abu Kasim, Noor Hayaty and Abu Kassim, Noor Lide and Rahman, Mohammad Tariqur (2017) Autologous serum supplement favours in vitro regenerative paracrine factors synthesis. Cell Proliferation, 50 (4). e12354-1. ISSN 0960-7722 http://onlinelibrary.wiley.com/doi/10.1111/cpr.12354/abstract 10.1111/cpr.12354
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
English
topic RC Internal medicine
RE Ophthalmology
RK Dentistry
spellingShingle RC Internal medicine
RE Ophthalmology
RK Dentistry
Haque, Nazmul
Abu Kasim, Noor Hayaty
Abu Kassim, Noor Lide
Rahman, Mohammad Tariqur
Autologous serum supplement favours in vitro regenerative paracrine factors synthesis
description Objectives: Foetal bovine serum (FBS) is often the serum supplement of choice for in vitro human cell culture. This study compares the effect of FBS and autologous human serum (AuHS) supplement in human peripheral blood mononuclear cell (PBMC) culture to prepare secretome. Materials and methods: The PBMC (n = 7) were cultured either in RPMI-1640 containing L-glutamine and 50 units/ml Penicillin-Streptomycin (BM) or in BM with either AuHS or FBS. Viability, proliferation and differentiation of PBMC were evaluated. Paracrine factors present in the secretomes (n = 6) were analysed using ProcartaPlex Human Cytokine panel (17 plex). Ingenuity Pathway Analysis (IPA) was performed to predict activation or inhibition of biological functions related to tissue regeneration. Results: The viability of PBMC that were cultured with FBS supplement was significantly reduced at 96 h compared to those at 0 and 24 h (P <.05). While the reduction of the viability of PBMC that were cultured with AuHS supplement was not significantly different compared to those at 0 and 24 h. The FBS secretomes prepared at 24 h was found to contain significantly higher amount of EGF (P <.05) compared to that in AuHS or BM secretome. The AuHS secretomes contained significantly higher amount of HGF at 24 (P <.05) and 96 h (P <.01), and VEGF-A at 24 h (P <.05) compared to those in the FBS secretomes. SDF-1 was not detected in the FBS secretomes prepared at either 24 or 96 hours. Double immunocytochemical staining revealed a marked increase in co-localization of SDF-1 and its receptor in PBMC that were cultured with AuHS supplement compared to that cultured with FBS supplement. Conclusion: In secretome preparation, AuHS supplement favours synthesis of paracrine factors that are needed for regenerative therapy.
format Article
author Haque, Nazmul
Abu Kasim, Noor Hayaty
Abu Kassim, Noor Lide
Rahman, Mohammad Tariqur
author_facet Haque, Nazmul
Abu Kasim, Noor Hayaty
Abu Kassim, Noor Lide
Rahman, Mohammad Tariqur
author_sort Haque, Nazmul
title Autologous serum supplement favours in vitro regenerative paracrine factors synthesis
title_short Autologous serum supplement favours in vitro regenerative paracrine factors synthesis
title_full Autologous serum supplement favours in vitro regenerative paracrine factors synthesis
title_fullStr Autologous serum supplement favours in vitro regenerative paracrine factors synthesis
title_full_unstemmed Autologous serum supplement favours in vitro regenerative paracrine factors synthesis
title_sort autologous serum supplement favours in vitro regenerative paracrine factors synthesis
publisher Wiley-Blackwell
publishDate 2017
url http://irep.iium.edu.my/59216/1/59216_Autologous%20serum%20supplement%20favours_article.pdf
http://irep.iium.edu.my/59216/2/59216_Autologous%20serum%20supplement%20favours_scopus.pdf
http://irep.iium.edu.my/59216/13/59216_Autologous%20serum%20supplement%20favours_ABSTRACT.pdf
http://irep.iium.edu.my/59216/
http://onlinelibrary.wiley.com/doi/10.1111/cpr.12354/abstract
_version_ 1643616955634024448
score 13.211869

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