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Establishment of growth kinetics profile and measurement of Sulphated Glycosaminoglycans (sGAG) production in monolayer cultured chondrocytes following Qur’anic recitation exposure

Objectives/Research Problem: Cartilage is an avascular tissue made of one cell type only which is the chondrocyte. Due to the low mitotic property, the chondrocytes have very limited self-repair capacity. As a result, once the cartilage is injured and left untreated, degeneration changes will prec...

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Main Authors: Rosyafirah Hashim, Rosyafirah, Shaban, Munirah, Zainuddin, Zainul Ibrahim
Format: Article
Language:English
Published: Kulliyyah of Allied Health Sciences, International Islamic University Malaysia 2017
Subjects:
BP Islam. Bahaism. Theosophy, etc
Q Science (General)
Online Access:http://irep.iium.edu.my/58523/14/58523.pdf
http://irep.iium.edu.my/58523/
http://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/71
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spelling my.iium.irep.585232017-09-29T07:34:45Z http://irep.iium.edu.my/58523/ Establishment of growth kinetics profile and measurement of Sulphated Glycosaminoglycans (sGAG) production in monolayer cultured chondrocytes following Qur’anic recitation exposure Rosyafirah Hashim, Rosyafirah Shaban, Munirah Zainuddin, Zainul Ibrahim BP Islam. Bahaism. Theosophy, etc Q Science (General) Objectives/Research Problem: Cartilage is an avascular tissue made of one cell type only which is the chondrocyte. Due to the low mitotic property, the chondrocytes have very limited self-repair capacity. As a result, once the cartilage is injured and left untreated, degeneration changes will precede ageing and can be progressive. Limitation in the currently available treatments is noted. Seeking alternative ways to facilitate the cartilage repair and regeneration have become crucial. This study aims to identify the potential effects of the Qur’anic recitation, particularly Surah Al-Fatihah on the sGAG production in the monolayer cultured chondrocytes derived from rabbit articular cartilage. Materials and Method: A cellular model based on a serially cultured and expanded chondrocytes is established in vitro and divided into four groups. The first group is exposed to the recitation of Surah Al-Fatihah. The second and third groups are exposed to the recitation of Arabic poem and Western poem respectively. The fourth group is not exposed to any sound and serves as control. Any significant changes are recorded and presented as photomicrographs. Growth kinetics assessment is performed to study the cell proliferation activities within each group. After reaching 80-90% confluency, the cells are harvested and pelleted through centrifugation step. The cell pellet is subjected to sGAG assay at different passages (P0, P1, P2, and P3). Results and Discussion: The cells exposed to Surah Al-Fatihah is expected to increase the proliferation and sGAG production of the chondrocytes better than the control group as well as the cells exposed to Arabic and Western poem recitation. Conclusion: Initial findings suggest that the Qur’anic recitation promotes cells proliferation and sGAG production. The Qur’anic recitation may serve as one of the potential signalling factors in tissue engineering studies and facilitate for cartilage repair and regeneration. Kulliyyah of Allied Health Sciences, International Islamic University Malaysia 2017-09-16 Article REM application/pdf en http://irep.iium.edu.my/58523/14/58523.pdf Rosyafirah Hashim, Rosyafirah and Shaban, Munirah and Zainuddin, Zainul Ibrahim (2017) Establishment of growth kinetics profile and measurement of Sulphated Glycosaminoglycans (sGAG) production in monolayer cultured chondrocytes following Qur’anic recitation exposure. International Journal Of Allied Health Sciences, 1 (1). (Unpublished) http://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/71
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic BP Islam. Bahaism. Theosophy, etc
Q Science (General)
spellingShingle BP Islam. Bahaism. Theosophy, etc
Q Science (General)
Rosyafirah Hashim, Rosyafirah
Shaban, Munirah
Zainuddin, Zainul Ibrahim
Establishment of growth kinetics profile and measurement of Sulphated Glycosaminoglycans (sGAG) production in monolayer cultured chondrocytes following Qur’anic recitation exposure
description Objectives/Research Problem: Cartilage is an avascular tissue made of one cell type only which is the chondrocyte. Due to the low mitotic property, the chondrocytes have very limited self-repair capacity. As a result, once the cartilage is injured and left untreated, degeneration changes will precede ageing and can be progressive. Limitation in the currently available treatments is noted. Seeking alternative ways to facilitate the cartilage repair and regeneration have become crucial. This study aims to identify the potential effects of the Qur’anic recitation, particularly Surah Al-Fatihah on the sGAG production in the monolayer cultured chondrocytes derived from rabbit articular cartilage. Materials and Method: A cellular model based on a serially cultured and expanded chondrocytes is established in vitro and divided into four groups. The first group is exposed to the recitation of Surah Al-Fatihah. The second and third groups are exposed to the recitation of Arabic poem and Western poem respectively. The fourth group is not exposed to any sound and serves as control. Any significant changes are recorded and presented as photomicrographs. Growth kinetics assessment is performed to study the cell proliferation activities within each group. After reaching 80-90% confluency, the cells are harvested and pelleted through centrifugation step. The cell pellet is subjected to sGAG assay at different passages (P0, P1, P2, and P3). Results and Discussion: The cells exposed to Surah Al-Fatihah is expected to increase the proliferation and sGAG production of the chondrocytes better than the control group as well as the cells exposed to Arabic and Western poem recitation. Conclusion: Initial findings suggest that the Qur’anic recitation promotes cells proliferation and sGAG production. The Qur’anic recitation may serve as one of the potential signalling factors in tissue engineering studies and facilitate for cartilage repair and regeneration.
format Article
author Rosyafirah Hashim, Rosyafirah
Shaban, Munirah
Zainuddin, Zainul Ibrahim
author_facet Rosyafirah Hashim, Rosyafirah
Shaban, Munirah
Zainuddin, Zainul Ibrahim
author_sort Rosyafirah Hashim, Rosyafirah
title Establishment of growth kinetics profile and measurement of Sulphated Glycosaminoglycans (sGAG) production in monolayer cultured chondrocytes following Qur’anic recitation exposure
title_short Establishment of growth kinetics profile and measurement of Sulphated Glycosaminoglycans (sGAG) production in monolayer cultured chondrocytes following Qur’anic recitation exposure
title_full Establishment of growth kinetics profile and measurement of Sulphated Glycosaminoglycans (sGAG) production in monolayer cultured chondrocytes following Qur’anic recitation exposure
title_fullStr Establishment of growth kinetics profile and measurement of Sulphated Glycosaminoglycans (sGAG) production in monolayer cultured chondrocytes following Qur’anic recitation exposure
title_full_unstemmed Establishment of growth kinetics profile and measurement of Sulphated Glycosaminoglycans (sGAG) production in monolayer cultured chondrocytes following Qur’anic recitation exposure
title_sort establishment of growth kinetics profile and measurement of sulphated glycosaminoglycans (sgag) production in monolayer cultured chondrocytes following qur’anic recitation exposure
publisher Kulliyyah of Allied Health Sciences, International Islamic University Malaysia
publishDate 2017
url http://irep.iium.edu.my/58523/14/58523.pdf
http://irep.iium.edu.my/58523/
http://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/71
_version_ 1643615374552334336
score 13.211869

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