Staff View: Polyamines deficient diet is a complementary strategy in fighting cancer: an investigation on selected medicinal fruits

Polyamines deficient diet is a complementary strategy in fighting cancer: an investigation on selected medicinal fruits

Polyamines are one of the oldest substances known in biochemistry consist of putrescine, spermidine and spermine. They are essential components of all living cells primarily involve in cell growth and proliferation. Mammalian cells are able to synthesize polyamines but are predominantly acquire them...

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Main Authors:	Abdul Ghani, Radiah, Jamil, Elyna Fatini, Mohd. Sajungi, Nur Hikmah, Aznan, Azni Masturah
Format:	Conference or Workshop Item
Language:	English English
Published:	2017
Subjects:	RB Pathology RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/58036/2/PRESENTATION%20ASIA%20711.pdf http://irep.iium.edu.my/58036/13/58036_Polyamines%20Deficient%20Diet.pdf http://irep.iium.edu.my/58036/ http://www.utm.my/asia/files/2017/05/2017-05-05-AIMC-2017-LSO.pdf
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id	my.iium.irep.58036
record_format	dspace
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English English
topic	RB Pathology RM Therapeutics. Pharmacology
spellingShingle	RB Pathology RM Therapeutics. Pharmacology Abdul Ghani, Radiah Jamil, Elyna Fatini Mohd. Sajungi, Nur Hikmah Aznan, Azni Masturah Polyamines deficient diet is a complementary strategy in fighting cancer: an investigation on selected medicinal fruits
description	Polyamines are one of the oldest substances known in biochemistry consist of putrescine, spermidine and spermine. They are essential components of all living cells primarily involve in cell growth and proliferation. Mammalian cells are able to synthesize polyamines but are predominantly acquire them from exogenous sources such as intestinal flora and diets. However, the dietary sources provide a larger quantity of polyamines than the endogenous biosynthesis. Polyamines are tightly regulated by systematic biosynthesis and catabolism. Dysregulation of this system is highly associated with various diseases, including cancer. Therefore, polyamines deficient has become one of the strategies to inhibit carcinogenesis by targeting each biosynthetic and catabolic step in polyamines metabolism. Polyamine deficient diet (PDD) is a part of nutritional cancer therapy that recommends foods with low polyamines intake by cancer survivor and patients. In some cases, the plants might show anticancer effect but since the polyamines is high, it should not recommend for patients to avoid recurrence of cancer. Thus, this study was aimed to determine the polyamines content in selected anticancer fruits and plants in Malaysia and the effect on polyamine biosynthetic and catabolic enzymes was explored using human lung carcinoma cells (A549) as in vitro model. Methods: In this study, the selected fruits and plants are Phoenix dactylifera (ajwa dates), Beta vulgaris (beetroot), Ziziphus jujube (jujube), Vitis vinifera (raisin) and Punica granatum (pomegranate). The selected fruits and plants were initially grouped into classes based on total polyamines content using High performance Liquid Chromatography (HPLC). The ability of selected fruits to reduce A549 intracellular polyamines was quantified using the same technique. Accordingly, changes in polyamine metabolic enzymes activities; ornithine decarboxylase (ODC) and spermidine/spermine N-acetyltransferase (SSAT) were measured correspondingly using quantitative polymerase chain reactions (qPCR). Results: Results suggested polyamines content of selected fruits were ranging from low to high polyamines . Beetroot, ajwa dates, pomegranate and raisin are classified as low polyamines fruits while jujube demonstrate high content of polyamines (219.6 ± 4.43) nmol/mg. Significant decreased of total intracellular polyamine content after 24 h of treatments with selected fruits were identified when compared with untreated A549 cells (p<0.001). Ornithine decarboxylase activity showed changes upon treatment with P. dactylifera (ajwa dates), Z. jujube (jujube) and V. vinifera (raisin) while SSAT activity displayed alterations in B. vulgaris (beetroot) and Z. jujube (jujube) treated cells. Thus, we concluded that B. vulgaris (beetroot), P. dactylifera (ajwa dates) and V. vinifera (raisins) are the promising candidates for food-based chemoprevention strategy for their low polyamines and ability to reduce A549 intracellular polyamines. Conclusion and Significant of Study: The studies above strengthened the potential of polyamine reduced diet in cancer prevention. The polyamine deficient diet strategy is being documented as complementary step in chemoprevention in other countries. In United Kingdom, USA, Japan and Sweden, the database and investigation on local food and herbs has been established and being practiced. It is hope that this finding is beneficial for cancer patient, cancer survivor and even high risk of population to get cancer via genetic alteration and environmental exposure. We have investigated several local plants in Malaysia, however for this report, we focus on selected medicinal fruits as recommended by Rasulullah SAW.
format	Conference or Workshop Item
author	Abdul Ghani, Radiah Jamil, Elyna Fatini Mohd. Sajungi, Nur Hikmah Aznan, Azni Masturah
author_facet	Abdul Ghani, Radiah Jamil, Elyna Fatini Mohd. Sajungi, Nur Hikmah Aznan, Azni Masturah
author_sort	Abdul Ghani, Radiah
title	Polyamines deficient diet is a complementary strategy in fighting cancer: an investigation on selected medicinal fruits
title_short	Polyamines deficient diet is a complementary strategy in fighting cancer: an investigation on selected medicinal fruits
title_full	Polyamines deficient diet is a complementary strategy in fighting cancer: an investigation on selected medicinal fruits
title_fullStr	Polyamines deficient diet is a complementary strategy in fighting cancer: an investigation on selected medicinal fruits
title_full_unstemmed	Polyamines deficient diet is a complementary strategy in fighting cancer: an investigation on selected medicinal fruits
title_sort	polyamines deficient diet is a complementary strategy in fighting cancer: an investigation on selected medicinal fruits
publishDate	2017
url	http://irep.iium.edu.my/58036/2/PRESENTATION%20ASIA%20711.pdf http://irep.iium.edu.my/58036/13/58036_Polyamines%20Deficient%20Diet.pdf http://irep.iium.edu.my/58036/ http://www.utm.my/asia/files/2017/05/2017-05-05-AIMC-2017-LSO.pdf
_version_	1643615271467876352
spelling	my.iium.irep.580362017-08-21T01:43:25Z http://irep.iium.edu.my/58036/ Polyamines deficient diet is a complementary strategy in fighting cancer: an investigation on selected medicinal fruits Abdul Ghani, Radiah Jamil, Elyna Fatini Mohd. Sajungi, Nur Hikmah Aznan, Azni Masturah RB Pathology RM Therapeutics. Pharmacology Polyamines are one of the oldest substances known in biochemistry consist of putrescine, spermidine and spermine. They are essential components of all living cells primarily involve in cell growth and proliferation. Mammalian cells are able to synthesize polyamines but are predominantly acquire them from exogenous sources such as intestinal flora and diets. However, the dietary sources provide a larger quantity of polyamines than the endogenous biosynthesis. Polyamines are tightly regulated by systematic biosynthesis and catabolism. Dysregulation of this system is highly associated with various diseases, including cancer. Therefore, polyamines deficient has become one of the strategies to inhibit carcinogenesis by targeting each biosynthetic and catabolic step in polyamines metabolism. Polyamine deficient diet (PDD) is a part of nutritional cancer therapy that recommends foods with low polyamines intake by cancer survivor and patients. In some cases, the plants might show anticancer effect but since the polyamines is high, it should not recommend for patients to avoid recurrence of cancer. Thus, this study was aimed to determine the polyamines content in selected anticancer fruits and plants in Malaysia and the effect on polyamine biosynthetic and catabolic enzymes was explored using human lung carcinoma cells (A549) as in vitro model. Methods: In this study, the selected fruits and plants are Phoenix dactylifera (ajwa dates), Beta vulgaris (beetroot), Ziziphus jujube (jujube), Vitis vinifera (raisin) and Punica granatum (pomegranate). The selected fruits and plants were initially grouped into classes based on total polyamines content using High performance Liquid Chromatography (HPLC). The ability of selected fruits to reduce A549 intracellular polyamines was quantified using the same technique. Accordingly, changes in polyamine metabolic enzymes activities; ornithine decarboxylase (ODC) and spermidine/spermine N-acetyltransferase (SSAT) were measured correspondingly using quantitative polymerase chain reactions (qPCR). Results: Results suggested polyamines content of selected fruits were ranging from low to high polyamines . Beetroot, ajwa dates, pomegranate and raisin are classified as low polyamines fruits while jujube demonstrate high content of polyamines (219.6 ± 4.43) nmol/mg. Significant decreased of total intracellular polyamine content after 24 h of treatments with selected fruits were identified when compared with untreated A549 cells (p<0.001). Ornithine decarboxylase activity showed changes upon treatment with P. dactylifera (ajwa dates), Z. jujube (jujube) and V. vinifera (raisin) while SSAT activity displayed alterations in B. vulgaris (beetroot) and Z. jujube (jujube) treated cells. Thus, we concluded that B. vulgaris (beetroot), P. dactylifera (ajwa dates) and V. vinifera (raisins) are the promising candidates for food-based chemoprevention strategy for their low polyamines and ability to reduce A549 intracellular polyamines. Conclusion and Significant of Study: The studies above strengthened the potential of polyamine reduced diet in cancer prevention. The polyamine deficient diet strategy is being documented as complementary step in chemoprevention in other countries. In United Kingdom, USA, Japan and Sweden, the database and investigation on local food and herbs has been established and being practiced. It is hope that this finding is beneficial for cancer patient, cancer survivor and even high risk of population to get cancer via genetic alteration and environmental exposure. We have investigated several local plants in Malaysia, however for this report, we focus on selected medicinal fruits as recommended by Rasulullah SAW. 2017-05-04 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/58036/2/PRESENTATION%20ASIA%20711.pdf application/pdf en http://irep.iium.edu.my/58036/13/58036_Polyamines%20Deficient%20Diet.pdf Abdul Ghani, Radiah and Jamil, Elyna Fatini and Mohd. Sajungi, Nur Hikmah and Aznan, Azni Masturah (2017) Polyamines deficient diet is a complementary strategy in fighting cancer: an investigation on selected medicinal fruits. In: ASIA International Multidisciplinary Conference 2017, 1st-2nd May 2017, Johor Bharu, Johor. (Unpublished) http://www.utm.my/asia/files/2017/05/2017-05-05-AIMC-2017-LSO.pdf
score	13.211869

Polyamines deficient diet is a complementary strategy in fighting cancer: an investigation on selected medicinal fruits

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