Investigation on plasmid DNA separation under vacuum suction using cellulose generated and polyethersulfone ultra filters
E. coli DH5α harbouring pET28a+ plasmid vector was lysed by alkaline lysis and the clarified plasmid solutions were subjected to ultrafiltration experiments using two types of ultrafiltration membrane; i.e., cellulose generated (Ultracel) and polyethersulfome PES (Biomax). Transmembrane pressures...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English English |
| Published: |
Open science Publisher
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/57890/1/Japs_Tg_Affaro.pdf http://irep.iium.edu.my/57890/7/57890_Investigation%20on%20plasmid%20DNA%20separation_SCOPUS.pdf http://irep.iium.edu.my/57890/ http://www.japsonline.com/admin/php/uploads/2327_pdf.pdf |
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| Summary: | E. coli DH5α harbouring pET28a+ plasmid vector was lysed by alkaline lysis and the clarified plasmid solutions
were subjected to ultrafiltration experiments using two types of ultrafiltration membrane; i.e., cellulose
generated (Ultracel) and polyethersulfome PES (Biomax). Transmembrane pressures (TMP) through vacuum
suction of 0.1 to 0.7 bar were applied to the plasmid sample, and the corresponding flow rates and fluxes for
both filters were investigated. Even though, these two filters showed a slight different in the flux, a marked
different in DNA transmission were observed. DNA transmission were generally higher with cellulose generated
filters, whereby DNA transmission by Ultracel filter was at44.3, 63.9, 74.1, 55.6 and 54.5% at TMP of 0.1, 0.18,
0.39, 0.6 and 0.7 bar, respectively. Meanwhile, for PES filters the DNA transmission was at 22.3, 38.1, 39.1,
38.0 and 37.5% at TMP of 0.1, 0.3, 0.4, 0.6 and 0.7 bar, respectively. With the cellulose generated filter, DNA
transmission reached an optimum (~70%) at about 0.6 bar after which the transmission depleted at higher TMP
of 0.7 bar. Throughout all of the TMP, DNA transmissions observed were generally lower with PES filter. The
properties of the filter material could have contributed to the differences in DNA permeation |
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