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The in vitro release study of orthopaedic metal implant coated with silver composite – Preliminary result

INTRODUCTION: The management of implant-infection poses a major challenge in orthopaedic field. Colonization of bacterial and biofilm adhesion on implant may lead to infection at underlying bone and surrounding tissues. Prolonged used of antimicrobial treatment with high dosage can lead to systemi...

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Main Authors: Mohd Jan, Nurul Hafiza, Zulkifly, Ahmad Hafiz, Ibrahim, Mohd Zulfadzli, Shaban, Munirah
Format: Conference or Workshop Item
Language:English
Published: 2017
Subjects:
RD701 Orthopedics
Online Access:http://irep.iium.edu.my/57851/31/57851.pdf
http://irep.iium.edu.my/57851/
http://www.apoatrauma.com/eposters.html
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spelling my.iium.irep.578512017-08-04T01:23:50Z http://irep.iium.edu.my/57851/ The in vitro release study of orthopaedic metal implant coated with silver composite – Preliminary result Mohd Jan, Nurul Hafiza Zulkifly, Ahmad Hafiz Ibrahim, Mohd Zulfadzli Shaban, Munirah RD701 Orthopedics INTRODUCTION: The management of implant-infection poses a major challenge in orthopaedic field. Colonization of bacterial and biofilm adhesion on implant may lead to infection at underlying bone and surrounding tissues. Prolonged used of antimicrobial treatment with high dosage can lead to systemic toxicity. Hence, to overcome this, alternative treatment strategy using orthopaedic metallic implant coated with silver composite as antibacterial agent have been introduced. It exhibits good bactericidal properties for biomedical application. Hence, the aim of this study is to investigate the potential of silver coated release of orthopaedic metal implant through direct current magnetron sputtering technique against biofilm-producing Staphylococcus aureus through in vitro experimental setting. METHODS: This method was employed to determine the release of silver as antibacterial agent. The coated plate was soaked with 200ml of Phosphate Buffer Saline (PBS) in shaking waterbath at 370C for silver extract preparation. A total of 5.0ml of solution in each assessment at 1 hour, 3 hours, 24 hours, 48 hours, 168 hours, 336 hours and 504 hours of time point were withdrawn for analyze. The bacterial inoculation was smeared on sterile nutrient agar media. A total of 20µl of 5.0ml of silver release solution in each assessment was loaded on the disc. A total of 20µl of Phosphate Buffer Saline (PBS) was loaded on the disk which served as control. The samples then were incubated with 370C for 24 hours for bacterial culture under aerobic condition. The sample then was continued incubated up to 168 hours with 370C under aerobic condition for assessment. The antibacterial effect was evaluated based on the diameter of inhibition zone using disk diffusion test. RESULTS: The colony was noted on the surface on nutrient agar after 24 hours of incubation. However, the inhibition zone was noted with diameter more than 20mm at 168 hours time point of silver extraction after 168 hours of incubation. The colonies number increase was noted at other silver extraction of time points. DISCUSSIONS: The use of orthopaedic implants has increase dramatically in all subspecialties in orthopaedic field. Recently, the coating of implants has engendered much interest in order to prevent adverse tissue reactions such as infection, inflammation, the foreign body response. Ewald A et al stated that silver coatings can inhibit the bacterial growth effectively. The early outcome of this study revealed positive indicator of the silver as antibacterial agent. The experiment work is still ongoing. CONCLUSION: This result suggested that orthopaedic metal implant coated with silver composite may provide antibacterial protection against medical device-related infection. 2017 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/57851/31/57851.pdf Mohd Jan, Nurul Hafiza and Zulkifly, Ahmad Hafiz and Ibrahim, Mohd Zulfadzli and Shaban, Munirah (2017) The in vitro release study of orthopaedic metal implant coated with silver composite – Preliminary result. In: Asia Pacific Orthopaedic Association Trauma and Infection Meeting 2017, 31st March - 2nd April 2017, Putrajaya. (Unpublished) http://www.apoatrauma.com/eposters.html
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic RD701 Orthopedics
spellingShingle RD701 Orthopedics
Mohd Jan, Nurul Hafiza
Zulkifly, Ahmad Hafiz
Ibrahim, Mohd Zulfadzli
Shaban, Munirah
The in vitro release study of orthopaedic metal implant coated with silver composite – Preliminary result
description INTRODUCTION: The management of implant-infection poses a major challenge in orthopaedic field. Colonization of bacterial and biofilm adhesion on implant may lead to infection at underlying bone and surrounding tissues. Prolonged used of antimicrobial treatment with high dosage can lead to systemic toxicity. Hence, to overcome this, alternative treatment strategy using orthopaedic metallic implant coated with silver composite as antibacterial agent have been introduced. It exhibits good bactericidal properties for biomedical application. Hence, the aim of this study is to investigate the potential of silver coated release of orthopaedic metal implant through direct current magnetron sputtering technique against biofilm-producing Staphylococcus aureus through in vitro experimental setting. METHODS: This method was employed to determine the release of silver as antibacterial agent. The coated plate was soaked with 200ml of Phosphate Buffer Saline (PBS) in shaking waterbath at 370C for silver extract preparation. A total of 5.0ml of solution in each assessment at 1 hour, 3 hours, 24 hours, 48 hours, 168 hours, 336 hours and 504 hours of time point were withdrawn for analyze. The bacterial inoculation was smeared on sterile nutrient agar media. A total of 20µl of 5.0ml of silver release solution in each assessment was loaded on the disc. A total of 20µl of Phosphate Buffer Saline (PBS) was loaded on the disk which served as control. The samples then were incubated with 370C for 24 hours for bacterial culture under aerobic condition. The sample then was continued incubated up to 168 hours with 370C under aerobic condition for assessment. The antibacterial effect was evaluated based on the diameter of inhibition zone using disk diffusion test. RESULTS: The colony was noted on the surface on nutrient agar after 24 hours of incubation. However, the inhibition zone was noted with diameter more than 20mm at 168 hours time point of silver extraction after 168 hours of incubation. The colonies number increase was noted at other silver extraction of time points. DISCUSSIONS: The use of orthopaedic implants has increase dramatically in all subspecialties in orthopaedic field. Recently, the coating of implants has engendered much interest in order to prevent adverse tissue reactions such as infection, inflammation, the foreign body response. Ewald A et al stated that silver coatings can inhibit the bacterial growth effectively. The early outcome of this study revealed positive indicator of the silver as antibacterial agent. The experiment work is still ongoing. CONCLUSION: This result suggested that orthopaedic metal implant coated with silver composite may provide antibacterial protection against medical device-related infection.
format Conference or Workshop Item
author Mohd Jan, Nurul Hafiza
Zulkifly, Ahmad Hafiz
Ibrahim, Mohd Zulfadzli
Shaban, Munirah
author_facet Mohd Jan, Nurul Hafiza
Zulkifly, Ahmad Hafiz
Ibrahim, Mohd Zulfadzli
Shaban, Munirah
author_sort Mohd Jan, Nurul Hafiza
title The in vitro release study of orthopaedic metal implant coated with silver composite – Preliminary result
title_short The in vitro release study of orthopaedic metal implant coated with silver composite – Preliminary result
title_full The in vitro release study of orthopaedic metal implant coated with silver composite – Preliminary result
title_fullStr The in vitro release study of orthopaedic metal implant coated with silver composite – Preliminary result
title_full_unstemmed The in vitro release study of orthopaedic metal implant coated with silver composite – Preliminary result
title_sort in vitro release study of orthopaedic metal implant coated with silver composite – preliminary result
publishDate 2017
url http://irep.iium.edu.my/57851/31/57851.pdf
http://irep.iium.edu.my/57851/
http://www.apoatrauma.com/eposters.html
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score 13.251813

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