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Screen printed electromechanical micro-total analysis system (μtas) for sensitive and rapid detection of infectious diseases

The main objective of this article is to demonstrate by performing simulation measurements of biosensor that can detect the presence of pathogens through simultaneous mass and impedance techniques. This biosensor merges two biosensing techniques namely resonant frequency measurements and electrochem...

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Main Authors: Nordin, Anis Nurashikin, Zainuddin, Ahmad Anwar, Ab Rahim, Rosminazuin, Voiculescu, Ioana R., Mak, Wing Cheung
Format: Article
Language:English
Published: Elsevier B.V. 2017
Subjects:
TK Electrical engineering. Electronics Nuclear engineering
Online Access:http://irep.iium.edu.my/57594/1/1-s2.0-S2212017317300440-main.pdf
http://irep.iium.edu.my/57594/
http://www.sciencedirect.com/science/article/pii/S2212017317300440
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id my.iium.irep.57594
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institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TK Electrical engineering. Electronics Nuclear engineering
spellingShingle TK Electrical engineering. Electronics Nuclear engineering
Nordin, Anis Nurashikin
Zainuddin, Ahmad Anwar
Ab Rahim, Rosminazuin
Voiculescu, Ioana R.
Mak, Wing Cheung
Screen printed electromechanical micro-total analysis system (μtas) for sensitive and rapid detection of infectious diseases
description The main objective of this article is to demonstrate by performing simulation measurements of biosensor that can detect the presence of pathogens through simultaneous mass and impedance techniques. This biosensor merges two biosensing techniques namely resonant frequency measurements and electrochemical impedance spectroscopy (EIS) on a single biosensor. Parallel measurements provide better sensitivities, have higher diagnostics accuracy and reduce the risk of false positives. Low cost, high resolution screen printing technology was used to fabricate the microelectromechanical array of μTAS on flexible piezoelectric substrates. The basic biosensor framework includes a substrate that highly sensitive sensor like thickness shear mode and immunosensor can be fabricated using quartz crystal lattice that integrated with electrochemical sensor [1]. The quartz crystal microbalance is a label free technique, which minimizes interference with the interaction being studied. A piezoelectric device is portable, simple and cost effective, and is suitable for real-time monitoring of biospecific interactions such as antigen-antibody, receptor ligand, and enzymes-substrate interactions with high sensitivity and specificity. For instance, the biological mixtures such as antibodies are capable of binding to terminal active functional groups (i.e., COOH, OH and NH2) of self-assembled monolayers (SAM) and immunocapture antigens such as glycoproptien or other targets[2]. The QCM can consequently detect mass changes due to these molecular interactions on the surface of the QCM. The top and bottom circular excitation electrodes with 150um diameter were modeled as gold (Au) films of 16μm thickness. A sinusoidal voltage with amplitude of 5 mV was applied across the quartz crystal. Figure 1 shows the principle of integrated biosensors which gold electrodes were printed on both sides of a thin 500um quartz layer to form the quartz crystal microbalance (QCM)-impedance device. The silver (Ag) semicircular counter electrode was modeled around the top working electrode on the same area of the quartz crystal for performing the electrochemical impedance spectroscopy (EIS) experiments for detection of bacteria (E-Coli) and the results were compared to quartz crystal microbalance measurements. Furthermore, the use of gold surface can be incorporated into the transducer compatible with the biological samples such as use of highly specific monoclonal antibodies, and incorporation of amplification step to maximize the signal detection. In general, the quartz crystal is traditionally considered to be a mass sensitive sensor that produces response which it changes its resonance frequency to different thin film samples or liquids in contact with it surface. For a straight relationship between a thin film mass of the order of nanograms, the quartz crystal response will be of of the order of Hertz according to Eq. 1, Sauerbrey Equation [3]. ρq and μq are the specific density and the shear modulus in quartz, respectively. ϝ0 is the fundamental resonance frequency in quartz, related to its thickness, nq. ∆m is the thin film mass deposited A, is the piezoelectrically active crystal area and n is the overtone number. Based on Eq. 1, it can be found that if the density of QCM changes, the resonant frequency of the device also changes, making the QCM suitable for monitoring changes in mass.
format Article
author Nordin, Anis Nurashikin
Zainuddin, Ahmad Anwar
Ab Rahim, Rosminazuin
Voiculescu, Ioana R.
Mak, Wing Cheung
author_facet Nordin, Anis Nurashikin
Zainuddin, Ahmad Anwar
Ab Rahim, Rosminazuin
Voiculescu, Ioana R.
Mak, Wing Cheung
author_sort Nordin, Anis Nurashikin
title Screen printed electromechanical micro-total analysis system (μtas) for sensitive and rapid detection of infectious diseases
title_short Screen printed electromechanical micro-total analysis system (μtas) for sensitive and rapid detection of infectious diseases
title_full Screen printed electromechanical micro-total analysis system (μtas) for sensitive and rapid detection of infectious diseases
title_fullStr Screen printed electromechanical micro-total analysis system (μtas) for sensitive and rapid detection of infectious diseases
title_full_unstemmed Screen printed electromechanical micro-total analysis system (μtas) for sensitive and rapid detection of infectious diseases
title_sort screen printed electromechanical micro-total analysis system (μtas) for sensitive and rapid detection of infectious diseases
publisher Elsevier B.V.
publishDate 2017
url http://irep.iium.edu.my/57594/1/1-s2.0-S2212017317300440-main.pdf
http://irep.iium.edu.my/57594/
http://www.sciencedirect.com/science/article/pii/S2212017317300440
_version_ 1643615179125030912
spelling my.iium.irep.575942018-01-11T06:02:45Z http://irep.iium.edu.my/57594/ Screen printed electromechanical micro-total analysis system (μtas) for sensitive and rapid detection of infectious diseases Nordin, Anis Nurashikin Zainuddin, Ahmad Anwar Ab Rahim, Rosminazuin Voiculescu, Ioana R. Mak, Wing Cheung TK Electrical engineering. Electronics Nuclear engineering The main objective of this article is to demonstrate by performing simulation measurements of biosensor that can detect the presence of pathogens through simultaneous mass and impedance techniques. This biosensor merges two biosensing techniques namely resonant frequency measurements and electrochemical impedance spectroscopy (EIS) on a single biosensor. Parallel measurements provide better sensitivities, have higher diagnostics accuracy and reduce the risk of false positives. Low cost, high resolution screen printing technology was used to fabricate the microelectromechanical array of μTAS on flexible piezoelectric substrates. The basic biosensor framework includes a substrate that highly sensitive sensor like thickness shear mode and immunosensor can be fabricated using quartz crystal lattice that integrated with electrochemical sensor [1]. The quartz crystal microbalance is a label free technique, which minimizes interference with the interaction being studied. A piezoelectric device is portable, simple and cost effective, and is suitable for real-time monitoring of biospecific interactions such as antigen-antibody, receptor ligand, and enzymes-substrate interactions with high sensitivity and specificity. For instance, the biological mixtures such as antibodies are capable of binding to terminal active functional groups (i.e., COOH, OH and NH2) of self-assembled monolayers (SAM) and immunocapture antigens such as glycoproptien or other targets[2]. The QCM can consequently detect mass changes due to these molecular interactions on the surface of the QCM. The top and bottom circular excitation electrodes with 150um diameter were modeled as gold (Au) films of 16μm thickness. A sinusoidal voltage with amplitude of 5 mV was applied across the quartz crystal. Figure 1 shows the principle of integrated biosensors which gold electrodes were printed on both sides of a thin 500um quartz layer to form the quartz crystal microbalance (QCM)-impedance device. The silver (Ag) semicircular counter electrode was modeled around the top working electrode on the same area of the quartz crystal for performing the electrochemical impedance spectroscopy (EIS) experiments for detection of bacteria (E-Coli) and the results were compared to quartz crystal microbalance measurements. Furthermore, the use of gold surface can be incorporated into the transducer compatible with the biological samples such as use of highly specific monoclonal antibodies, and incorporation of amplification step to maximize the signal detection. In general, the quartz crystal is traditionally considered to be a mass sensitive sensor that produces response which it changes its resonance frequency to different thin film samples or liquids in contact with it surface. For a straight relationship between a thin film mass of the order of nanograms, the quartz crystal response will be of of the order of Hertz according to Eq. 1, Sauerbrey Equation [3]. ρq and μq are the specific density and the shear modulus in quartz, respectively. ϝ0 is the fundamental resonance frequency in quartz, related to its thickness, nq. ∆m is the thin film mass deposited A, is the piezoelectrically active crystal area and n is the overtone number. Based on Eq. 1, it can be found that if the density of QCM changes, the resonant frequency of the device also changes, making the QCM suitable for monitoring changes in mass. Elsevier B.V. 2017 Article REM application/pdf en http://irep.iium.edu.my/57594/1/1-s2.0-S2212017317300440-main.pdf Nordin, Anis Nurashikin and Zainuddin, Ahmad Anwar and Ab Rahim, Rosminazuin and Voiculescu, Ioana R. and Mak, Wing Cheung (2017) Screen printed electromechanical micro-total analysis system (μtas) for sensitive and rapid detection of infectious diseases. Procedia Technology, 27. pp. 101-102. ISSN 2212-0173 http://www.sciencedirect.com/science/article/pii/S2212017317300440 10.1016/j.protcy.2017.04.043
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