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The isolation and identification of Acanthamoeba from air ventilation system samples in selected buildings in Kuantan

Acanthamoeba, a free-living amoeba (FLA) which is abundant in a wide range of the environmental conditions including water, soil and air. Since Acanthamoeba is opportunistic pathogen, some of its species are found to be responsible for serious human diseases. Cysts and trophozoites of Acanthamoeba m...

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Main Authors: Aznan, Anis Amira, Mat Yusof, Afzan, Md Isa, Muhammad Lokman, Mat Amin, Nakisah
Format: Conference or Workshop Item
Language:English
Published: 2017
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/57566/1/Conference%20Malaysian%20Symposium%202017%20a.pdf
http://irep.iium.edu.my/57566/
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spelling my.iium.irep.575662017-07-13T02:00:10Z http://irep.iium.edu.my/57566/ The isolation and identification of Acanthamoeba from air ventilation system samples in selected buildings in Kuantan Aznan, Anis Amira Mat Yusof, Afzan Md Isa, Muhammad Lokman Mat Amin, Nakisah Q Science (General) Acanthamoeba, a free-living amoeba (FLA) which is abundant in a wide range of the environmental conditions including water, soil and air. Since Acanthamoeba is opportunistic pathogen, some of its species are found to be responsible for serious human diseases. Cysts and trophozoites of Acanthamoeba may be deposited in air ventilation systems with maintaining practices. This could predispose discomfort and diseases to human health especially towards immunosuppressed individuals. Acanthamoeba was isolated from dust samples taken from air ventilation systems in selected buildings in Kuantan. Acanthamoeba was isolated from dust samples taken from air ventilation systems in selected buildings in Kuantan. By using a sterile swab, the dust samples were collected from filters of the air ventilation systems. The dust samples were cultured on the non-nutrient agar plates with inactivate Escherichia coli and incubated in 37ºC incubator. The plates were examined daily for any presence of Acanthamoeba cysts up to 14 days of incubation. The results showed that all 75 dust samples collected were negative. The microscopic examination showed that all non-nutrient agar (NNA) plates were negative for Acantamoeba from day 1 until day 14 of the incubation period. However, fungal growth was observed on the non-nutrient agar (NNA) plates after day 4 of incubation. The negative findings of this study may have been influenced by the environmental factor in Kuantan. Besides that, there are also limitations in the method employed in this study where direct swabs from air ventilation systems was were used instead of using a more specific method such as establishing proper sampling points with air quality instruments to collect air samples. Thus, further studies should consider in order to address these limitations and conditions. 2017-05-12 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/57566/1/Conference%20Malaysian%20Symposium%202017%20a.pdf Aznan, Anis Amira and Mat Yusof, Afzan and Md Isa, Muhammad Lokman and Mat Amin, Nakisah (2017) The isolation and identification of Acanthamoeba from air ventilation system samples in selected buildings in Kuantan. In: 8th Malaysian Symposium of Biomedical Science, 12th-14th May 2017, Kuantan, Pahang Darul Makmur. (Unpublished)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Aznan, Anis Amira
Mat Yusof, Afzan
Md Isa, Muhammad Lokman
Mat Amin, Nakisah
The isolation and identification of Acanthamoeba from air ventilation system samples in selected buildings in Kuantan
description Acanthamoeba, a free-living amoeba (FLA) which is abundant in a wide range of the environmental conditions including water, soil and air. Since Acanthamoeba is opportunistic pathogen, some of its species are found to be responsible for serious human diseases. Cysts and trophozoites of Acanthamoeba may be deposited in air ventilation systems with maintaining practices. This could predispose discomfort and diseases to human health especially towards immunosuppressed individuals. Acanthamoeba was isolated from dust samples taken from air ventilation systems in selected buildings in Kuantan. Acanthamoeba was isolated from dust samples taken from air ventilation systems in selected buildings in Kuantan. By using a sterile swab, the dust samples were collected from filters of the air ventilation systems. The dust samples were cultured on the non-nutrient agar plates with inactivate Escherichia coli and incubated in 37ºC incubator. The plates were examined daily for any presence of Acanthamoeba cysts up to 14 days of incubation. The results showed that all 75 dust samples collected were negative. The microscopic examination showed that all non-nutrient agar (NNA) plates were negative for Acantamoeba from day 1 until day 14 of the incubation period. However, fungal growth was observed on the non-nutrient agar (NNA) plates after day 4 of incubation. The negative findings of this study may have been influenced by the environmental factor in Kuantan. Besides that, there are also limitations in the method employed in this study where direct swabs from air ventilation systems was were used instead of using a more specific method such as establishing proper sampling points with air quality instruments to collect air samples. Thus, further studies should consider in order to address these limitations and conditions.
format Conference or Workshop Item
author Aznan, Anis Amira
Mat Yusof, Afzan
Md Isa, Muhammad Lokman
Mat Amin, Nakisah
author_facet Aznan, Anis Amira
Mat Yusof, Afzan
Md Isa, Muhammad Lokman
Mat Amin, Nakisah
author_sort Aznan, Anis Amira
title The isolation and identification of Acanthamoeba from air ventilation system samples in selected buildings in Kuantan
title_short The isolation and identification of Acanthamoeba from air ventilation system samples in selected buildings in Kuantan
title_full The isolation and identification of Acanthamoeba from air ventilation system samples in selected buildings in Kuantan
title_fullStr The isolation and identification of Acanthamoeba from air ventilation system samples in selected buildings in Kuantan
title_full_unstemmed The isolation and identification of Acanthamoeba from air ventilation system samples in selected buildings in Kuantan
title_sort isolation and identification of acanthamoeba from air ventilation system samples in selected buildings in kuantan
publishDate 2017
url http://irep.iium.edu.my/57566/1/Conference%20Malaysian%20Symposium%202017%20a.pdf
http://irep.iium.edu.my/57566/
_version_ 1643615172548362240
score 13.211869

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