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Optimization of signalling biomarkers in detecting male infertility

Objectives/Research Problem: To detect potential signals of fertility biomarkers from non-invasive samples in male reproductive function after X-ray and light exposure to Sprague-Dawley rats. Materials and Method: Male Sprague-Dawley rats with each group (n=6) were exposed to whole body irradiation...

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Main Authors:	Zahri, Syazana, Abdul Rahman, Suzanah, Abdul Majid, Zafri Azran, Ahmad, Nur Amalina, Abdul Samat, Nadia Hanis, Abdullah, 'Afif Raihan
Format:	Conference or Workshop Item
Language:	English English
Published:	2016
Subjects:	RG Gynecology and obstetrics RM Therapeutics. Pharmacology RM695 Physical medicine. Physical therapy
Online Access:	http://irep.iium.edu.my/56844/1/KRW%202016_Syazana.pdf http://irep.iium.edu.my/56844/2/Syazana%20Mohamad%20Zahri%20%28Poster%29.pdf http://irep.iium.edu.my/56844/
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spelling	my.iium.irep.568442017-05-17T01:01:35Z http://irep.iium.edu.my/56844/ Optimization of signalling biomarkers in detecting male infertility Zahri, Syazana Abdul Rahman, Suzanah Abdul Majid, Zafri Azran Ahmad, Nur Amalina Abdul Samat, Nadia Hanis Abdullah, 'Afif Raihan RG Gynecology and obstetrics RM Therapeutics. Pharmacology RM695 Physical medicine. Physical therapy Objectives/Research Problem: To detect potential signals of fertility biomarkers from non-invasive samples in male reproductive function after X-ray and light exposure to Sprague-Dawley rats. Materials and Method: Male Sprague-Dawley rats with each group (n=6) were exposed to whole body irradiation to high (2.5Gy), medium (0.2Gy) and low (0.05Gy) doses of X-ray. Another group were exposed to continuous light to induce stress. Positive control group were treated with cisplatin and corticosterone while negative control group was left with no intervention. Sampling of blood, saliva and urine will be done prior to exposure and 56 days after exposure. These hormones level in the samples was measured using enzyme-linked immunosorbent assay (ELISA) kit. Histological assessment of the testes and spermatozoa quality were assessed using haematoxylin and eosin (H&E) stain and spermatozoa quality such as sperm count and sperm motility were done together with acridine orange staining to assess DNA. Using the saliva and urine samples, specific signals were captured by transducers, amplified and processed to capture data that reflects the hormone levels. The signals were compared and optimized to produce sensitive tools. Results and Discussion: The expected results was that the X-ray and prolonged light exposure can affect testosterone level that showed reduced man fertility. The presence of this biomarkers in urine and saliva samples can be used to develop diagnostic device/kit. Conclusion: Development of biosensor using significant biomarkers in potentially non-invasive samples can be used in management of infertility. 2016 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/56844/1/KRW%202016_Syazana.pdf application/pdf en http://irep.iium.edu.my/56844/2/Syazana%20Mohamad%20Zahri%20%28Poster%29.pdf Zahri, Syazana and Abdul Rahman, Suzanah and Abdul Majid, Zafri Azran and Ahmad, Nur Amalina and Abdul Samat, Nadia Hanis and Abdullah, 'Afif Raihan (2016) Optimization of signalling biomarkers in detecting male infertility. In: KAHS Research Week (KRW) 2016 in conjunction with 1st Allied Health Scientific Colloquium (AHSC) 2016, 21st - 25th November 2016, Kuantan, Pahang. (Unpublished)
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English English
topic	RG Gynecology and obstetrics RM Therapeutics. Pharmacology RM695 Physical medicine. Physical therapy
spellingShingle	RG Gynecology and obstetrics RM Therapeutics. Pharmacology RM695 Physical medicine. Physical therapy Zahri, Syazana Abdul Rahman, Suzanah Abdul Majid, Zafri Azran Ahmad, Nur Amalina Abdul Samat, Nadia Hanis Abdullah, 'Afif Raihan Optimization of signalling biomarkers in detecting male infertility
description	Objectives/Research Problem: To detect potential signals of fertility biomarkers from non-invasive samples in male reproductive function after X-ray and light exposure to Sprague-Dawley rats. Materials and Method: Male Sprague-Dawley rats with each group (n=6) were exposed to whole body irradiation to high (2.5Gy), medium (0.2Gy) and low (0.05Gy) doses of X-ray. Another group were exposed to continuous light to induce stress. Positive control group were treated with cisplatin and corticosterone while negative control group was left with no intervention. Sampling of blood, saliva and urine will be done prior to exposure and 56 days after exposure. These hormones level in the samples was measured using enzyme-linked immunosorbent assay (ELISA) kit. Histological assessment of the testes and spermatozoa quality were assessed using haematoxylin and eosin (H&E) stain and spermatozoa quality such as sperm count and sperm motility were done together with acridine orange staining to assess DNA. Using the saliva and urine samples, specific signals were captured by transducers, amplified and processed to capture data that reflects the hormone levels. The signals were compared and optimized to produce sensitive tools. Results and Discussion: The expected results was that the X-ray and prolonged light exposure can affect testosterone level that showed reduced man fertility. The presence of this biomarkers in urine and saliva samples can be used to develop diagnostic device/kit. Conclusion: Development of biosensor using significant biomarkers in potentially non-invasive samples can be used in management of infertility.
format	Conference or Workshop Item
author	Zahri, Syazana Abdul Rahman, Suzanah Abdul Majid, Zafri Azran Ahmad, Nur Amalina Abdul Samat, Nadia Hanis Abdullah, 'Afif Raihan
author_facet	Zahri, Syazana Abdul Rahman, Suzanah Abdul Majid, Zafri Azran Ahmad, Nur Amalina Abdul Samat, Nadia Hanis Abdullah, 'Afif Raihan
author_sort	Zahri, Syazana
title	Optimization of signalling biomarkers in detecting male infertility
title_short	Optimization of signalling biomarkers in detecting male infertility
title_full	Optimization of signalling biomarkers in detecting male infertility
title_fullStr	Optimization of signalling biomarkers in detecting male infertility
title_full_unstemmed	Optimization of signalling biomarkers in detecting male infertility
title_sort	optimization of signalling biomarkers in detecting male infertility
publishDate	2016
url	http://irep.iium.edu.my/56844/1/KRW%202016_Syazana.pdf http://irep.iium.edu.my/56844/2/Syazana%20Mohamad%20Zahri%20%28Poster%29.pdf http://irep.iium.edu.my/56844/
_version_	1643615009420345344
score	13.211869

Optimization of signalling biomarkers in detecting male infertility

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