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The effect of particle size, methods and time of extraction on the natural antioxidant content and radical scavenging activity of Phyllanthus niruri

Introduction: Phyllanthus niruri has high antioxidant capacity due to high phenolic and flavonoid content. This study is to determine and evaluate the effect of particle size, methods and time of extraction on its antioxidant activities. Methods: P. niruri was dried overnight in drier at 40˚C and gr...

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Main Authors: Muhamad Bunnori, Noraslinda, Darnis, Deny Susanti, Yusof, Muhamad Najib, Abd Halim, Khairul Bariyyah
Format: Conference or Workshop Item
Language:English
Published: 2016
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/56714/12/THE%20EFFECT%20OF%20PARTICLE%20SIZE_%20new.pdf
http://irep.iium.edu.my/56714/
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spelling my.iium.irep.567142017-05-03T04:07:12Z http://irep.iium.edu.my/56714/ The effect of particle size, methods and time of extraction on the natural antioxidant content and radical scavenging activity of Phyllanthus niruri Muhamad Bunnori, Noraslinda Darnis, Deny Susanti Yusof, Muhamad Najib Abd Halim, Khairul Bariyyah Q Science (General) Introduction: Phyllanthus niruri has high antioxidant capacity due to high phenolic and flavonoid content. This study is to determine and evaluate the effect of particle size, methods and time of extraction on its antioxidant activities. Methods: P. niruri was dried overnight in drier at 40˚C and grounded to different particle sizes. The extraction methods involved were soxhlet and maceration. Three types of biochemical assays were conducted: total phenolic content (TPC), total flavonoids content (TFC) and 1,1-Diphenyl-2 picrylhydrazyl (DPPH) radical scaveging activity assay. Result: The best TPC and DPPH value is recorded for the sample of less than 0.7 mm, treated by soxhlet extraction method at 8 hours extraction time with TPC valued at 4.95 ± 0.803 mg GAE/g of dried extract and IC50 value of 0.403 ± 0.03790 mg/mL respectively. The highest TFC amount is recorded for the sample with less than 0.7 mm particle size, by maceration method of extraction at 8 hours extraction time with the total flavonoid content of 56.43 ± 0.594 mg QE/g of dried extract. Conclusion: It can be concluded that there are a synergistic effect between TPC and TFC on the quality of the anti-radical property of the sample affecting DPPH activity within the sample. 2016-08-04 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/56714/12/THE%20EFFECT%20OF%20PARTICLE%20SIZE_%20new.pdf Muhamad Bunnori, Noraslinda and Darnis, Deny Susanti and Yusof, Muhamad Najib and Abd Halim, Khairul Bariyyah (2016) The effect of particle size, methods and time of extraction on the natural antioxidant content and radical scavenging activity of Phyllanthus niruri. In: Medical Research Symposium & Pacific Partnership in conjunction with Kuantan Research Day 2016, 4th April 2016, Kuantan, Pahang. (Unpublished)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Muhamad Bunnori, Noraslinda
Darnis, Deny Susanti
Yusof, Muhamad Najib
Abd Halim, Khairul Bariyyah
The effect of particle size, methods and time of extraction on the natural antioxidant content and radical scavenging activity of Phyllanthus niruri
description Introduction: Phyllanthus niruri has high antioxidant capacity due to high phenolic and flavonoid content. This study is to determine and evaluate the effect of particle size, methods and time of extraction on its antioxidant activities. Methods: P. niruri was dried overnight in drier at 40˚C and grounded to different particle sizes. The extraction methods involved were soxhlet and maceration. Three types of biochemical assays were conducted: total phenolic content (TPC), total flavonoids content (TFC) and 1,1-Diphenyl-2 picrylhydrazyl (DPPH) radical scaveging activity assay. Result: The best TPC and DPPH value is recorded for the sample of less than 0.7 mm, treated by soxhlet extraction method at 8 hours extraction time with TPC valued at 4.95 ± 0.803 mg GAE/g of dried extract and IC50 value of 0.403 ± 0.03790 mg/mL respectively. The highest TFC amount is recorded for the sample with less than 0.7 mm particle size, by maceration method of extraction at 8 hours extraction time with the total flavonoid content of 56.43 ± 0.594 mg QE/g of dried extract. Conclusion: It can be concluded that there are a synergistic effect between TPC and TFC on the quality of the anti-radical property of the sample affecting DPPH activity within the sample.
format Conference or Workshop Item
author Muhamad Bunnori, Noraslinda
Darnis, Deny Susanti
Yusof, Muhamad Najib
Abd Halim, Khairul Bariyyah
author_facet Muhamad Bunnori, Noraslinda
Darnis, Deny Susanti
Yusof, Muhamad Najib
Abd Halim, Khairul Bariyyah
author_sort Muhamad Bunnori, Noraslinda
title The effect of particle size, methods and time of extraction on the natural antioxidant content and radical scavenging activity of Phyllanthus niruri
title_short The effect of particle size, methods and time of extraction on the natural antioxidant content and radical scavenging activity of Phyllanthus niruri
title_full The effect of particle size, methods and time of extraction on the natural antioxidant content and radical scavenging activity of Phyllanthus niruri
title_fullStr The effect of particle size, methods and time of extraction on the natural antioxidant content and radical scavenging activity of Phyllanthus niruri
title_full_unstemmed The effect of particle size, methods and time of extraction on the natural antioxidant content and radical scavenging activity of Phyllanthus niruri
title_sort effect of particle size, methods and time of extraction on the natural antioxidant content and radical scavenging activity of phyllanthus niruri
publishDate 2016
url http://irep.iium.edu.my/56714/12/THE%20EFFECT%20OF%20PARTICLE%20SIZE_%20new.pdf
http://irep.iium.edu.my/56714/
_version_ 1643614982938558464
score 13.211869

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