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Proteomics profiling of crassostrea iredalei exposed to toxic alexandrium tamiyavanichii and non-toxic alexandrium leei

The black scar oyster, Crassostrea iredalei is part of filter feeder organism and found extremely responsive to alterations in the environment from any natural contamination such as harmful algal blooms (HABs). The commercial demand of oyster is therefore of utmost importance to monitor the presence...

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Main Authors: Hamdan, Nurul Ashima, Mamat, Siti Noor Hasmiza, Abu Hassan, Muhamad Shafiq, Mohammad Noor, Normawaty, Muhamad Bunnori, Noraslinda
Format: Conference or Workshop Item
Language:English
English
English
Published: Emirates Research Publishing Limited, Dignified Researchers Publications 2017
Subjects:
S Agriculture (General)
Online Access:http://irep.iium.edu.my/56713/1/56713_paper.pdf
http://irep.iium.edu.my/56713/12/56713%20Proteomics%20Profiling.pdf
http://irep.iium.edu.my/56713/2/56713_slide.pdf
http://irep.iium.edu.my/56713/
http://dirpub.org/images/proceedings_pdf/DIR0417285.pdf
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spelling my.iium.irep.567132018-11-15T02:25:28Z http://irep.iium.edu.my/56713/ Proteomics profiling of crassostrea iredalei exposed to toxic alexandrium tamiyavanichii and non-toxic alexandrium leei Hamdan, Nurul Ashima Mamat, Siti Noor Hasmiza Abu Hassan, Muhamad Shafiq Mohammad Noor, Normawaty Muhamad Bunnori, Noraslinda S Agriculture (General) The black scar oyster, Crassostrea iredalei is part of filter feeder organism and found extremely responsive to alterations in the environment from any natural contamination such as harmful algal blooms (HABs). The commercial demand of oyster is therefore of utmost importance to monitor the presence and effect on related toxins at protein level. Hence, the current study involved the comparison in protein profiles of C. iredalei after exposed to toxic A. tamiyavanichi and non-toxic A. leei for 24-hour. This aimed is achievable using two-dimensional gel electrophoresis (2-DE) that can identify the differential abundances of proteins spots for oyster’s protein profiling. There were 12 spots detected in a pH range of 3-10 that can be predicted via cross-referring of the protein molecular weight and isoelectric points, pI in Expasy databases. These predicted proteins were involved in biological activities such as energy metabolism, cell regulation and stress response. Besides that, there were two proteins spots that have been expressed in the toxic sample and further identified using LCMS analysis. Both proteins; arginine kinase and transgelin, were highly expressed in intoxicated oyster due to the oxidative stress induced by the toxic microenvironment. The presence of A. tamiyavanichi caused toxin accumulation in oysters and might induce the disorder of energy metabolism. The regulation mechanism thus explains on the oyster stability during HAB outbreak. Emirates Research Publishing Limited, Dignified Researchers Publications 2017 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/56713/1/56713_paper.pdf application/pdf en http://irep.iium.edu.my/56713/12/56713%20Proteomics%20Profiling.pdf application/pdf en http://irep.iium.edu.my/56713/2/56713_slide.pdf Hamdan, Nurul Ashima and Mamat, Siti Noor Hasmiza and Abu Hassan, Muhamad Shafiq and Mohammad Noor, Normawaty and Muhamad Bunnori, Noraslinda (2017) Proteomics profiling of crassostrea iredalei exposed to toxic alexandrium tamiyavanichii and non-toxic alexandrium leei. In: 5th International Conference on Chemical, Agricultural, Biological and Environmental Sciences (CAFES-17), 18th-21st April 2017, Kyoto, Japan. http://dirpub.org/images/proceedings_pdf/DIR0417285.pdf 10.15242/DIRPUB.DIR0417285
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
English
topic S Agriculture (General)
spellingShingle S Agriculture (General)
Hamdan, Nurul Ashima
Mamat, Siti Noor Hasmiza
Abu Hassan, Muhamad Shafiq
Mohammad Noor, Normawaty
Muhamad Bunnori, Noraslinda
Proteomics profiling of crassostrea iredalei exposed to toxic alexandrium tamiyavanichii and non-toxic alexandrium leei
description The black scar oyster, Crassostrea iredalei is part of filter feeder organism and found extremely responsive to alterations in the environment from any natural contamination such as harmful algal blooms (HABs). The commercial demand of oyster is therefore of utmost importance to monitor the presence and effect on related toxins at protein level. Hence, the current study involved the comparison in protein profiles of C. iredalei after exposed to toxic A. tamiyavanichi and non-toxic A. leei for 24-hour. This aimed is achievable using two-dimensional gel electrophoresis (2-DE) that can identify the differential abundances of proteins spots for oyster’s protein profiling. There were 12 spots detected in a pH range of 3-10 that can be predicted via cross-referring of the protein molecular weight and isoelectric points, pI in Expasy databases. These predicted proteins were involved in biological activities such as energy metabolism, cell regulation and stress response. Besides that, there were two proteins spots that have been expressed in the toxic sample and further identified using LCMS analysis. Both proteins; arginine kinase and transgelin, were highly expressed in intoxicated oyster due to the oxidative stress induced by the toxic microenvironment. The presence of A. tamiyavanichi caused toxin accumulation in oysters and might induce the disorder of energy metabolism. The regulation mechanism thus explains on the oyster stability during HAB outbreak.
format Conference or Workshop Item
author Hamdan, Nurul Ashima
Mamat, Siti Noor Hasmiza
Abu Hassan, Muhamad Shafiq
Mohammad Noor, Normawaty
Muhamad Bunnori, Noraslinda
author_facet Hamdan, Nurul Ashima
Mamat, Siti Noor Hasmiza
Abu Hassan, Muhamad Shafiq
Mohammad Noor, Normawaty
Muhamad Bunnori, Noraslinda
author_sort Hamdan, Nurul Ashima
title Proteomics profiling of crassostrea iredalei exposed to toxic alexandrium tamiyavanichii and non-toxic alexandrium leei
title_short Proteomics profiling of crassostrea iredalei exposed to toxic alexandrium tamiyavanichii and non-toxic alexandrium leei
title_full Proteomics profiling of crassostrea iredalei exposed to toxic alexandrium tamiyavanichii and non-toxic alexandrium leei
title_fullStr Proteomics profiling of crassostrea iredalei exposed to toxic alexandrium tamiyavanichii and non-toxic alexandrium leei
title_full_unstemmed Proteomics profiling of crassostrea iredalei exposed to toxic alexandrium tamiyavanichii and non-toxic alexandrium leei
title_sort proteomics profiling of crassostrea iredalei exposed to toxic alexandrium tamiyavanichii and non-toxic alexandrium leei
publisher Emirates Research Publishing Limited, Dignified Researchers Publications
publishDate 2017
url http://irep.iium.edu.my/56713/1/56713_paper.pdf
http://irep.iium.edu.my/56713/12/56713%20Proteomics%20Profiling.pdf
http://irep.iium.edu.my/56713/2/56713_slide.pdf
http://irep.iium.edu.my/56713/
http://dirpub.org/images/proceedings_pdf/DIR0417285.pdf
_version_ 1643617764254941184
score 13.211869

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