Fast and simple gas chromatographic method for simultaneous estimation of camphor, menthol and methyl salicylate in analgesic ointment: application in stability study
A simple, rapid and sensitive gas chromatographic (GC) method with flame ionization detector (FID) has been developed and validated for simultaneous estimation of camphor, menthol and methyl salicylate (MS). Camphor, menthol and MS were separated at about 2.753, 3.206 and 3.995 min respectively...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English English |
| Published: |
SpringerLink
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/56687/1/GC%20JPI.pdf http://irep.iium.edu.my/56687/7/56687_Fast%20and%20simple%20gas%20chromatographic.pdf http://irep.iium.edu.my/56687/13/56687_Fast%20and%20simple%20gas%20chromatographic%20method_article.pdf http://irep.iium.edu.my/56687/ https://link.springer.com/article/10.1007%2Fs40005-017-0305-0 |
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| Summary: | A simple, rapid and sensitive gas chromatographic
(GC) method with flame ionization detector (FID)
has been developed and validated for simultaneous estimation
of camphor, menthol and methyl salicylate (MS).
Camphor, menthol and MS were separated at about 2.753,
3.206 and 3.995 min respectively on a capillary column
with helium (3.3 ml/min) as carrier gas within 11 min run
time. Noninterference of any peak with the peaks of interest
confirms the selectivity of method. Derived quantitation
limits (QL) were 0.847, 0.684 and 6.507 μg/ml for camphor,
menthol and MS respectively. The linear relationship
(
R2 > 0.999) between analyte concentration vs detector
response was established within a range of QL to 150%
of label claim concentration for each analyte. Recovery of
each analyte at 50, 100 and 150% of label claim concentration
levels were obtained within 99.67–101.53% establishing
high accuracy of the method. The method showed
acceptable precision with low relative standard deviation
or RSD (0.24–1.03%) between percent recoveries for each
analyte. RSD for intermediate precision (inter day analysis,
analyst variation) was less than 1%. The validated method
was successfully applied for quantitative determination of
camphor, menthol and MS in stability samples of an analgesic
ointment produced by IKOP Sdn. Bhd., Malaysia. |
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