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Mimosa pudica L.: A comparative study via in vitro analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol

The present study compared conventional (maceration) extraction (EtOHconv) and supercritical fluid extraction (SFE) methods as a mean of comparing conventional and green process for a weed namely Mimosa pudica L. to obtain a safe antidiabetic natural agent. In vitro analysis comprised of two differe...

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Main Authors: Tasnuva, S. T., Ferdosh, Sahena, Ahmed, Qamar Uddin, Kashif, G, Jafri, Juliana, Sarker, Md. Zaidul Islam
Format: Article
Language:English
English
English
Published: National Institute of Science Communication and Information Resources (NISCAIR) 2017
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/55726/1/55726-Mimosa%20pudica.pdf
http://irep.iium.edu.my/55726/2/55726-Mimosa%20pudica_SCOPUS.pdf
http://irep.iium.edu.my/55726/3/55726-Mimosa%20pudica_WOS.pdf
http://irep.iium.edu.my/55726/
http://nopr.niscair.res.in/bitstream/123456789/41452/1/IJNPR%208%281%29%2054-62.pdf
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spelling my.iium.irep.557262017-07-03T03:35:48Z http://irep.iium.edu.my/55726/ Mimosa pudica L.: A comparative study via in vitro analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol Tasnuva, S. T. Ferdosh, Sahena Ahmed, Qamar Uddin Kashif, G Jafri, Juliana Sarker, Md. Zaidul Islam Q Science (General) The present study compared conventional (maceration) extraction (EtOHconv) and supercritical fluid extraction (SFE) methods as a mean of comparing conventional and green process for a weed namely Mimosa pudica L. to obtain a safe antidiabetic natural agent. In vitro analysis comprised of two different assays, antioxidant assay (determination of total phenolic content, total flavonoid content, and 2,2-diphenyl-1-picrylhydrazyl assay) and antidiabetic assay (inhibition of α-amylase and α-glucosidase). GC Q-TOF MS profiling for both extracts was done after derivetisation to confirm the presence of bioactive compounds. SFE was performed at 40 MPa pressure, 60 °C temperature and 5 mL/min CO2 flow rate using 30 % ethanol (co-solvent) for 2 h. EtOHconv prepared using 95 % ethanol through conventional method (maceration) showed a good in vitro antioxidant potential and digestive enzymes inhibitory effect compared to supercritical fluid extract. α-amylase and α-glucosidase inhibitory activities for EtOHconv at 1 mg/mL were 30.08 % (±5.23) and 38.29 % (±2.52), whereas for standard acarbose it was 28.24 % (±13.66) and 36.93 % (±2.70), respectively. Supercritical fluid extract showed less potent in vitro antioxidant and digestive enzymes inhibitory effects (15.67±4.03- α-amylase, 28.36±2.01- α-glucosidase). GC Q-TOF MS analysis was done to confirm the presence of bioactive compounds in both the extracts. Although EtOHconv showed better results, SFE was found to contain more bioactive compounds associated with various pharmacological effects especially antioxidative as per GC Q-TOF MS results. SFE being a clean and green technology could be employed in future with more focus on method development and optimization to reproduce better and safe bioactive products from the neglected weed M. pudica. National Institute of Science Communication and Information Resources (NISCAIR) 2017-03 Article REM application/pdf en http://irep.iium.edu.my/55726/1/55726-Mimosa%20pudica.pdf application/pdf en http://irep.iium.edu.my/55726/2/55726-Mimosa%20pudica_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/55726/3/55726-Mimosa%20pudica_WOS.pdf Tasnuva, S. T. and Ferdosh, Sahena and Ahmed, Qamar Uddin and Kashif, G and Jafri, Juliana and Sarker, Md. Zaidul Islam (2017) Mimosa pudica L.: A comparative study via in vitro analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol. Indian Journal of Natural Products and Resources (IJNPR), 8 (1). pp. 54-62. ISSN 0976-0504 http://nopr.niscair.res.in/bitstream/123456789/41452/1/IJNPR%208%281%29%2054-62.pdf
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
English
topic Q Science (General)
spellingShingle Q Science (General)
Tasnuva, S. T.
Ferdosh, Sahena
Ahmed, Qamar Uddin
Kashif, G
Jafri, Juliana
Sarker, Md. Zaidul Islam
Mimosa pudica L.: A comparative study via in vitro analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol
description The present study compared conventional (maceration) extraction (EtOHconv) and supercritical fluid extraction (SFE) methods as a mean of comparing conventional and green process for a weed namely Mimosa pudica L. to obtain a safe antidiabetic natural agent. In vitro analysis comprised of two different assays, antioxidant assay (determination of total phenolic content, total flavonoid content, and 2,2-diphenyl-1-picrylhydrazyl assay) and antidiabetic assay (inhibition of α-amylase and α-glucosidase). GC Q-TOF MS profiling for both extracts was done after derivetisation to confirm the presence of bioactive compounds. SFE was performed at 40 MPa pressure, 60 °C temperature and 5 mL/min CO2 flow rate using 30 % ethanol (co-solvent) for 2 h. EtOHconv prepared using 95 % ethanol through conventional method (maceration) showed a good in vitro antioxidant potential and digestive enzymes inhibitory effect compared to supercritical fluid extract. α-amylase and α-glucosidase inhibitory activities for EtOHconv at 1 mg/mL were 30.08 % (±5.23) and 38.29 % (±2.52), whereas for standard acarbose it was 28.24 % (±13.66) and 36.93 % (±2.70), respectively. Supercritical fluid extract showed less potent in vitro antioxidant and digestive enzymes inhibitory effects (15.67±4.03- α-amylase, 28.36±2.01- α-glucosidase). GC Q-TOF MS analysis was done to confirm the presence of bioactive compounds in both the extracts. Although EtOHconv showed better results, SFE was found to contain more bioactive compounds associated with various pharmacological effects especially antioxidative as per GC Q-TOF MS results. SFE being a clean and green technology could be employed in future with more focus on method development and optimization to reproduce better and safe bioactive products from the neglected weed M. pudica.
format Article
author Tasnuva, S. T.
Ferdosh, Sahena
Ahmed, Qamar Uddin
Kashif, G
Jafri, Juliana
Sarker, Md. Zaidul Islam
author_facet Tasnuva, S. T.
Ferdosh, Sahena
Ahmed, Qamar Uddin
Kashif, G
Jafri, Juliana
Sarker, Md. Zaidul Islam
author_sort Tasnuva, S. T.
title Mimosa pudica L.: A comparative study via in vitro analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol
title_short Mimosa pudica L.: A comparative study via in vitro analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol
title_full Mimosa pudica L.: A comparative study via in vitro analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol
title_fullStr Mimosa pudica L.: A comparative study via in vitro analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol
title_full_unstemmed Mimosa pudica L.: A comparative study via in vitro analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol
title_sort mimosa pudica l.: a comparative study via in vitro analysis and gc q-tof ms profiling on conventional and supercritical fluid extraction using food grade ethanol
publisher National Institute of Science Communication and Information Resources (NISCAIR)
publishDate 2017
url http://irep.iium.edu.my/55726/1/55726-Mimosa%20pudica.pdf
http://irep.iium.edu.my/55726/2/55726-Mimosa%20pudica_SCOPUS.pdf
http://irep.iium.edu.my/55726/3/55726-Mimosa%20pudica_WOS.pdf
http://irep.iium.edu.my/55726/
http://nopr.niscair.res.in/bitstream/123456789/41452/1/IJNPR%208%281%29%2054-62.pdf
_version_ 1643614790008963072
score 13.211869

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