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Evaluation of genetic homogeneity of Jatropha curcas L. hybrid at an early stage of shoot bud formation from petioles using ISSR marker

Genetic homogeneity is known to be the most important prerequisite in the micropropagation of Jatropha curcas L. to produce true-to-type plants. The detection of genetic homogeneity in clonal micropropagation for elite plants at an early stage is required, to avoid any increase in variation in the n...

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Main Authors: Md. Nasir, Nor Anis Nadhirah, Anuar, Nurina, Zainuddin, Zarina, Yaakob, Zahira
Format: Article
Language:English
English
English
Published: Taylor & Francis Online 2017
Subjects:
SB Plant culture
Online Access:http://irep.iium.edu.my/55216/1/2017%20Anis%20-%20Evaluation%20of%20genetic%20homogeneity%20of%20Jatropha%20curcas%20L%20hybrid%20at%20an%20early%20stage%20of%20shoot%20bud%20formation%20from%20petioles%20using%20ISSR%20marker.pdf
http://irep.iium.edu.my/55216/7/55216_Evaluation%20of%20genetic%20homogeneity_WOS.pdf
http://irep.iium.edu.my/55216/8/55216_Evaluation%20of%20genetic%20homogeneity%20of%20Jatropha%20curcas_scopus.pdf
http://irep.iium.edu.my/55216/
http://www.tandfonline.com/loi/tbeq20
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spelling my.iium.irep.552162018-04-25T01:45:39Z http://irep.iium.edu.my/55216/ Evaluation of genetic homogeneity of Jatropha curcas L. hybrid at an early stage of shoot bud formation from petioles using ISSR marker Md. Nasir, Nor Anis Nadhirah Anuar, Nurina Zainuddin, Zarina Yaakob, Zahira SB Plant culture Genetic homogeneity is known to be the most important prerequisite in the micropropagation of Jatropha curcas L. to produce true-to-type plants. The detection of genetic homogeneity in clonal micropropagation for elite plants at an early stage is required, to avoid any increase in variation in the next stage of micropropagation. The genetic homogeneity was assessed during shoot bud formation from petiole explants of J.curcas (P1 £ P3) hybrid with different concentrations of thidiazuron (TDZ) in a range of 0.5–4.0 mg/L using inter simple sequence repeat (ISSR) markers. Out of 23 ISSR primers, 16 primers produced clear, distinct and reproducible bands. A total of 96 bands, ranging in size from 100 to 1013 bp were generated. Based on the band data, a total of 94 bands were monomorphic (98%) and two bands were polymorphic (2%). All banding patterns from the shoot buds induced by 0.5, 1.0 and 2.0 mg/L TDZ were monomorphic, but 4.0 mg/L gave 2% polymorphism. These findings indicated that concentrations of 0.5, 1.0 and 2.0 mg/L TDZ did not trigger any somaclonal variation and could, therefore, be considered suitable for application in clonal micropropagation of J. curcas hybrid using petioles as explant material. Taylor & Francis Online 2017-01 Article REM application/pdf en http://irep.iium.edu.my/55216/1/2017%20Anis%20-%20Evaluation%20of%20genetic%20homogeneity%20of%20Jatropha%20curcas%20L%20hybrid%20at%20an%20early%20stage%20of%20shoot%20bud%20formation%20from%20petioles%20using%20ISSR%20marker.pdf application/pdf en http://irep.iium.edu.my/55216/7/55216_Evaluation%20of%20genetic%20homogeneity_WOS.pdf application/pdf en http://irep.iium.edu.my/55216/8/55216_Evaluation%20of%20genetic%20homogeneity%20of%20Jatropha%20curcas_scopus.pdf Md. Nasir, Nor Anis Nadhirah and Anuar, Nurina and Zainuddin, Zarina and Yaakob, Zahira (2017) Evaluation of genetic homogeneity of Jatropha curcas L. hybrid at an early stage of shoot bud formation from petioles using ISSR marker. Biotechnology & Biotechnological Equipment, 31 (1). pp. 45-50. ISSN 1310-2818 http://www.tandfonline.com/loi/tbeq20 10.1080/13102818.2016.1257923
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
English
topic SB Plant culture
spellingShingle SB Plant culture
Md. Nasir, Nor Anis Nadhirah
Anuar, Nurina
Zainuddin, Zarina
Yaakob, Zahira
Evaluation of genetic homogeneity of Jatropha curcas L. hybrid at an early stage of shoot bud formation from petioles using ISSR marker
description Genetic homogeneity is known to be the most important prerequisite in the micropropagation of Jatropha curcas L. to produce true-to-type plants. The detection of genetic homogeneity in clonal micropropagation for elite plants at an early stage is required, to avoid any increase in variation in the next stage of micropropagation. The genetic homogeneity was assessed during shoot bud formation from petiole explants of J.curcas (P1 £ P3) hybrid with different concentrations of thidiazuron (TDZ) in a range of 0.5–4.0 mg/L using inter simple sequence repeat (ISSR) markers. Out of 23 ISSR primers, 16 primers produced clear, distinct and reproducible bands. A total of 96 bands, ranging in size from 100 to 1013 bp were generated. Based on the band data, a total of 94 bands were monomorphic (98%) and two bands were polymorphic (2%). All banding patterns from the shoot buds induced by 0.5, 1.0 and 2.0 mg/L TDZ were monomorphic, but 4.0 mg/L gave 2% polymorphism. These findings indicated that concentrations of 0.5, 1.0 and 2.0 mg/L TDZ did not trigger any somaclonal variation and could, therefore, be considered suitable for application in clonal micropropagation of J. curcas hybrid using petioles as explant material.
format Article
author Md. Nasir, Nor Anis Nadhirah
Anuar, Nurina
Zainuddin, Zarina
Yaakob, Zahira
author_facet Md. Nasir, Nor Anis Nadhirah
Anuar, Nurina
Zainuddin, Zarina
Yaakob, Zahira
author_sort Md. Nasir, Nor Anis Nadhirah
title Evaluation of genetic homogeneity of Jatropha curcas L. hybrid at an early stage of shoot bud formation from petioles using ISSR marker
title_short Evaluation of genetic homogeneity of Jatropha curcas L. hybrid at an early stage of shoot bud formation from petioles using ISSR marker
title_full Evaluation of genetic homogeneity of Jatropha curcas L. hybrid at an early stage of shoot bud formation from petioles using ISSR marker
title_fullStr Evaluation of genetic homogeneity of Jatropha curcas L. hybrid at an early stage of shoot bud formation from petioles using ISSR marker
title_full_unstemmed Evaluation of genetic homogeneity of Jatropha curcas L. hybrid at an early stage of shoot bud formation from petioles using ISSR marker
title_sort evaluation of genetic homogeneity of jatropha curcas l. hybrid at an early stage of shoot bud formation from petioles using issr marker
publisher Taylor & Francis Online
publishDate 2017
url http://irep.iium.edu.my/55216/1/2017%20Anis%20-%20Evaluation%20of%20genetic%20homogeneity%20of%20Jatropha%20curcas%20L%20hybrid%20at%20an%20early%20stage%20of%20shoot%20bud%20formation%20from%20petioles%20using%20ISSR%20marker.pdf
http://irep.iium.edu.my/55216/7/55216_Evaluation%20of%20genetic%20homogeneity_WOS.pdf
http://irep.iium.edu.my/55216/8/55216_Evaluation%20of%20genetic%20homogeneity%20of%20Jatropha%20curcas_scopus.pdf
http://irep.iium.edu.my/55216/
http://www.tandfonline.com/loi/tbeq20
_version_ 1643614700991152128
score 13.211869

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