Staff View: Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition

Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition

Swelling capacity and degradation behaviour are important factors for biomaterial scaffold in tissue engineering (TE). Poly(lactic-co-glycolic acid) (PLGA) is a synthetic polymer used commonly as scaffolding material. Major concern with PLGA use is the trigger of inflammatory reaction damaging cells...

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Main Authors:	Mohamad, Mohd Yusof, Mohamed Amin, Muhammad Azri Ifwat, Md Nazir, Noorhidayah, Ahmad Radzi, Muhammad Aa’zamuddin, Hashim, Rosyafirah, Zulkifly, Ahmad Hafiz, Sha'ban, Munirah
Format:	Conference or Workshop Item
Language:	English
Published:	UTM 2016
Subjects:	TA164 Bioengineering
Online Access:	http://irep.iium.edu.my/54992/1/Yusof%20ISPC%20Proceeding.pdf http://irep.iium.edu.my/54992/
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spelling	my.iium.irep.549922017-03-06T00:41:27Z http://irep.iium.edu.my/54992/ Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa’zamuddin Hashim, Rosyafirah Zulkifly, Ahmad Hafiz Sha'ban, Munirah TA164 Bioengineering Swelling capacity and degradation behaviour are important factors for biomaterial scaffold in tissue engineering (TE). Poly(lactic-co-glycolic acid) (PLGA) is a synthetic polymer used commonly as scaffolding material. Major concern with PLGA use is the trigger of inflammatory reaction damaging cells in vivo. Incorporation of natural polymers i.e. atelocollagen and/or fibrin onto PLGA may compensate those effects. This present study aimed to evaluate the swelling capacity and degradation behaviour of PLGA when added with atelocollagen, fibrin or, combination of both atelocollagen and fibrin. Atelocollagen type II has no telopeptide region and forms gel like structure at body temperature. Plasma-derived fibrin is reported to reduce inflammation caused by PLGA. Combination of both atelocollagen and fibrin could enhance PLGA properties. Porous PLGA scaffolds were fabricated via solvent-casting/salt-leaching method using salt as porogen. Atelocollagen was then added and cross-linked with the PLGA using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-Hydroxysuccinimide (NHS) to form PLGA/atelocollagen (PA) scaffold. Plasma-derived fibrin was added to the PLGA and the prefabricated PLGA/atelocollagen scaffold to form PLGA/fibrin (PF) and PLGA/atelocollagen/fibrin scaffold (PAF) respectively. Calcium chloride was used to polymerize plasma-derived fibrin onto those scaffolds. Swelling test was performed by immersing the scaffolds in water for 24-hour. Hydrolytic degradation was performed by immersing the scaffolds in simulated body fluid (SBF) and the percentage of reduction was recorded at 28 days. Four scaffolds groups i.e. (1) PLGA alone, (2) PLGA/atelocollagen [PA], (3) PLGA/fibrin [PF], and (4) PLGA/atelocollagen/fibrin [PAF] were compared in this study. The swelling ratio of PA scaffold group (192.57±41.96%) had significantly increased compared to other groups (PLGA= 40.19±4.89%; PF=52.00±23.48%; PAF=66.59±13.38%). In terms of degradation, PA group exhibited significant weight loss (497.11±76.65%) over other groups after 28 days in SBF. These findings suggest PLGA/atelocollagen without fibrin is sufficient to increase swelling capacity and offer higher degradation properties. UTM 2016-02-24 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/54992/1/Yusof%20ISPC%20Proceeding.pdf Mohamad, Mohd Yusof and Mohamed Amin, Muhammad Azri Ifwat and Md Nazir, Noorhidayah and Ahmad Radzi, Muhammad Aa’zamuddin and Hashim, Rosyafirah and Zulkifly, Ahmad Hafiz and Sha'ban, Munirah (2016) Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition. In: 4th International Science Postgraduate Conference 2016, 22-24 Feb 2016, Johor Bahru.
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English
topic	TA164 Bioengineering
spellingShingle	TA164 Bioengineering Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa’zamuddin Hashim, Rosyafirah Zulkifly, Ahmad Hafiz Sha'ban, Munirah Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition
description	Swelling capacity and degradation behaviour are important factors for biomaterial scaffold in tissue engineering (TE). Poly(lactic-co-glycolic acid) (PLGA) is a synthetic polymer used commonly as scaffolding material. Major concern with PLGA use is the trigger of inflammatory reaction damaging cells in vivo. Incorporation of natural polymers i.e. atelocollagen and/or fibrin onto PLGA may compensate those effects. This present study aimed to evaluate the swelling capacity and degradation behaviour of PLGA when added with atelocollagen, fibrin or, combination of both atelocollagen and fibrin. Atelocollagen type II has no telopeptide region and forms gel like structure at body temperature. Plasma-derived fibrin is reported to reduce inflammation caused by PLGA. Combination of both atelocollagen and fibrin could enhance PLGA properties. Porous PLGA scaffolds were fabricated via solvent-casting/salt-leaching method using salt as porogen. Atelocollagen was then added and cross-linked with the PLGA using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-Hydroxysuccinimide (NHS) to form PLGA/atelocollagen (PA) scaffold. Plasma-derived fibrin was added to the PLGA and the prefabricated PLGA/atelocollagen scaffold to form PLGA/fibrin (PF) and PLGA/atelocollagen/fibrin scaffold (PAF) respectively. Calcium chloride was used to polymerize plasma-derived fibrin onto those scaffolds. Swelling test was performed by immersing the scaffolds in water for 24-hour. Hydrolytic degradation was performed by immersing the scaffolds in simulated body fluid (SBF) and the percentage of reduction was recorded at 28 days. Four scaffolds groups i.e. (1) PLGA alone, (2) PLGA/atelocollagen [PA], (3) PLGA/fibrin [PF], and (4) PLGA/atelocollagen/fibrin [PAF] were compared in this study. The swelling ratio of PA scaffold group (192.57±41.96%) had significantly increased compared to other groups (PLGA= 40.19±4.89%; PF=52.00±23.48%; PAF=66.59±13.38%). In terms of degradation, PA group exhibited significant weight loss (497.11±76.65%) over other groups after 28 days in SBF. These findings suggest PLGA/atelocollagen without fibrin is sufficient to increase swelling capacity and offer higher degradation properties.
format	Conference or Workshop Item
author	Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa’zamuddin Hashim, Rosyafirah Zulkifly, Ahmad Hafiz Sha'ban, Munirah
author_facet	Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa’zamuddin Hashim, Rosyafirah Zulkifly, Ahmad Hafiz Sha'ban, Munirah
author_sort	Mohamad, Mohd Yusof
title	Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition
title_short	Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition
title_full	Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition
title_fullStr	Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition
title_full_unstemmed	Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition
title_sort	swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition
publisher	UTM
publishDate	2016
url	http://irep.iium.edu.my/54992/1/Yusof%20ISPC%20Proceeding.pdf http://irep.iium.edu.my/54992/
_version_	1643614655429476352
score	13.211869

Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition

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