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Purification of bioactive phenolics from Phanerochaete chysosporium biomass extract on selected macroporous resins

In this study, two different types of macroporous resins known as XAD-7HP and HP-20 were evaluated for the adsorption and desorption properties against bioactive phenolics extracted from Phanerochaete chrysosporium. For static sorption, the study were conducted at different phase contact time, solut...

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主要な著者: Idris, Zulkarnain Mohamed, Mohamed Dzahir, Mohd Irfan Hatim, Jamal, Parveen, Ahmad Barkat, Anumsima, Xian, R. L.W.
フォーマット: Conference or Workshop Item
言語:English
English
出版事項: IOP Publishing 2017
主題:
TP Chemical technology
TP248.13 Biotechnology
オンライン･アクセス:http://irep.iium.edu.my/54431/21/54431-Purification%20of%20bioactive%20phenolics.pdf
http://irep.iium.edu.my/54431/20/54431-Purification%20of%20bioactive%20phenolics-SCOPUS.pdf
http://irep.iium.edu.my/54431/
http://iopscience.iop.org/article/10.1088/1757-899X/206/1/012070/meta
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spelling my.iium.irep.54431 http://irep.iium.edu.my/54431/ Purification of bioactive phenolics from Phanerochaete chysosporium biomass extract on selected macroporous resins Idris, Zulkarnain Mohamed Mohamed Dzahir, Mohd Irfan Hatim Jamal, Parveen Ahmad Barkat, Anumsima Xian, R. L.W. TP Chemical technology TP248.13 Biotechnology In this study, two different types of macroporous resins known as XAD-7HP and HP-20 were evaluated for the adsorption and desorption properties against bioactive phenolics extracted from Phanerochaete chrysosporium. For static sorption, the study were conducted at different phase contact time, solution pH and ethanol concentration by static adsorption/ desorption method. From the results, it was found that the adsorption capacity for both resins had has no significant difference. Then, the kinetic adsorption data were analyzed with both pseudo-first-order and pseudo-second-order equations and the later performed better. The adsorption isotherm data were fitted well by both Langmuir and Freundlich models. Meanwhile in desorption study, HP-20 and XAD-7HP gave 90.52% and 88.28% recoveries, respectively. Considering the desorption results of the macroporous resins, HP-20 and XAD-7HP were packed in chromatography column to further purify the phenolics. For dynamic adsorption, breakthrough capacity of HP-20 (0.522) was found to be higher than XAD7HP (0.131). Different ethanol concentrations (30% to 50% (v/v)) were investigated at fixed flowrate (1 ml/min) on phenolics recovery from both types of resins. The highest recovery of bioactive phenolics was 94.3% using XAD-7HP resins at 50% (v/v) of ethanol. Only 77.1% of bioactive phenolics were recovered using HP-20 resin at the same experimental conditions. The purified extract subsequently was analyzed using HPLC. The results showed that three phenolics (gallic acid 3,4-dihydroxybenzoic acid and 4-hydroxybenzoic acid) were identified with higher concentrations as compared to non-purified extract. Finally, the purified extract was tested for scavenging activity against DPPH, and it showed that the activity increased significantly to 90.80% from 59.94% in non-purified extract. IOP Publishing 2017-06-21 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/54431/21/54431-Purification%20of%20bioactive%20phenolics.pdf application/pdf en http://irep.iium.edu.my/54431/20/54431-Purification%20of%20bioactive%20phenolics-SCOPUS.pdf Idris, Zulkarnain Mohamed and Mohamed Dzahir, Mohd Irfan Hatim and Jamal, Parveen and Ahmad Barkat, Anumsima and Xian, R. L.W. (2017) Purification of bioactive phenolics from Phanerochaete chysosporium biomass extract on selected macroporous resins. In: 29th Symposium of Malaysian Chemical Engineers (SOMChE 2016), 1st-3rd December 2016, Sarawak, Malaysia. http://iopscience.iop.org/article/10.1088/1757-899X/206/1/012070/meta 10.1088/1757-899X/206/1/012070
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic TP Chemical technology
TP248.13 Biotechnology
spellingShingle TP Chemical technology
TP248.13 Biotechnology
Idris, Zulkarnain Mohamed
Mohamed Dzahir, Mohd Irfan Hatim
Jamal, Parveen
Ahmad Barkat, Anumsima
Xian, R. L.W.
Purification of bioactive phenolics from Phanerochaete chysosporium biomass extract on selected macroporous resins
description In this study, two different types of macroporous resins known as XAD-7HP and HP-20 were evaluated for the adsorption and desorption properties against bioactive phenolics extracted from Phanerochaete chrysosporium. For static sorption, the study were conducted at different phase contact time, solution pH and ethanol concentration by static adsorption/ desorption method. From the results, it was found that the adsorption capacity for both resins had has no significant difference. Then, the kinetic adsorption data were analyzed with both pseudo-first-order and pseudo-second-order equations and the later performed better. The adsorption isotherm data were fitted well by both Langmuir and Freundlich models. Meanwhile in desorption study, HP-20 and XAD-7HP gave 90.52% and 88.28% recoveries, respectively. Considering the desorption results of the macroporous resins, HP-20 and XAD-7HP were packed in chromatography column to further purify the phenolics. For dynamic adsorption, breakthrough capacity of HP-20 (0.522) was found to be higher than XAD7HP (0.131). Different ethanol concentrations (30% to 50% (v/v)) were investigated at fixed flowrate (1 ml/min) on phenolics recovery from both types of resins. The highest recovery of bioactive phenolics was 94.3% using XAD-7HP resins at 50% (v/v) of ethanol. Only 77.1% of bioactive phenolics were recovered using HP-20 resin at the same experimental conditions. The purified extract subsequently was analyzed using HPLC. The results showed that three phenolics (gallic acid 3,4-dihydroxybenzoic acid and 4-hydroxybenzoic acid) were identified with higher concentrations as compared to non-purified extract. Finally, the purified extract was tested for scavenging activity against DPPH, and it showed that the activity increased significantly to 90.80% from 59.94% in non-purified extract.
format Conference or Workshop Item
author Idris, Zulkarnain Mohamed
Mohamed Dzahir, Mohd Irfan Hatim
Jamal, Parveen
Ahmad Barkat, Anumsima
Xian, R. L.W.
author_facet Idris, Zulkarnain Mohamed
Mohamed Dzahir, Mohd Irfan Hatim
Jamal, Parveen
Ahmad Barkat, Anumsima
Xian, R. L.W.
author_sort Idris, Zulkarnain Mohamed
title Purification of bioactive phenolics from Phanerochaete chysosporium biomass extract on selected macroporous resins
title_short Purification of bioactive phenolics from Phanerochaete chysosporium biomass extract on selected macroporous resins
title_full Purification of bioactive phenolics from Phanerochaete chysosporium biomass extract on selected macroporous resins
title_fullStr Purification of bioactive phenolics from Phanerochaete chysosporium biomass extract on selected macroporous resins
title_full_unstemmed Purification of bioactive phenolics from Phanerochaete chysosporium biomass extract on selected macroporous resins
title_sort purification of bioactive phenolics from phanerochaete chysosporium biomass extract on selected macroporous resins
publisher IOP Publishing
publishDate 2017
url http://irep.iium.edu.my/54431/21/54431-Purification%20of%20bioactive%20phenolics.pdf
http://irep.iium.edu.my/54431/20/54431-Purification%20of%20bioactive%20phenolics-SCOPUS.pdf
http://irep.iium.edu.my/54431/
http://iopscience.iop.org/article/10.1088/1757-899X/206/1/012070/meta
_version_ 1643616931973955584
score 13.250246

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