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A preliminary study on the effects of radiation exposure on chondrocytes:: Sulphated Glycosaminoglycan (sGAG) content using cellular model

Radiation is known to be harmful to human health. However, the effect of radiation at cellular level has yet to be established. This study evaluated the possible effects of radiation on monolayer cultured chondrocytes. Rabbit’s chondrocytes were isolated, serially cultured and divided into two grou...

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Main Authors: Mat Nawi, Nur Farhana, Zainuddin, Zainul Ibrahim, Sha'ban, Munirah
Format: Conference or Workshop Item
Language:English
English
Published: 2016
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/53948/1/NurFarhana.PANASIAN2016.pdf
http://irep.iium.edu.my/53948/2/NurFarhana.PANASIAN2016_Poster.pdf
http://irep.iium.edu.my/53948/
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spelling my.iium.irep.539482016-12-26T04:45:32Z http://irep.iium.edu.my/53948/ A preliminary study on the effects of radiation exposure on chondrocytes:: Sulphated Glycosaminoglycan (sGAG) content using cellular model Mat Nawi, Nur Farhana Zainuddin, Zainul Ibrahim Sha'ban, Munirah Q Science (General) Radiation is known to be harmful to human health. However, the effect of radiation at cellular level has yet to be established. This study evaluated the possible effects of radiation on monolayer cultured chondrocytes. Rabbit’s chondrocytes were isolated, serially cultured and divided into two groups; (1) non-irradiated and (2) irradiated. Group 2 cells were subjected to irradiation after it reaches 80-90% confluency. Both groups 1 and 2 cells were taken out from the incubator, placed into a temperature-controlled container and were transported to a typical X-ray examination room. After irradiating group 2, both cell groups were returned into the incubator. Both groups were evaluated using morphological evaluation and sulphated glycosaminoglycan (sGAG) production at passages 0, 1, 2, and 3. All groups exhibited comparable morphological appearances throughout the passages. Both cell groups experienced gradual changes from chondrocytic to fibroblastic morphology. The non-irradiated group showed a decreasing trend of sGAG concentration after each successive passage. It is suggestive that when the optimum proliferating conditions of these cells were manipulated, stress was induced in the cells. Meanwhile, the irradiated group showed an increasing trend in relative sGAG concentration after each passage. This particular phenomenon is unexpected in view of the known harmful nature associated with radiation. This study suggested that the introduction of radiation exposure could have certain effects on the cartilaginous extracellular matrix (ECM) production. It is postulated that the induced radiation created a yet to be explained situation, where the cells tend to recover, regenerate and secrete extracellular matrix. Basically, this finding warrants further evaluation where gene expression and histological analysis will be conducted. The limitation of study includes the number of samples used (n=2). Future work involving more samples and subjecting the cells to irradiation up to passage 5 will be conducted. Keywords: Glycosaminoglycan; cartilage; chondrocyte; radiation; X-ray; cellular model 2016-12-07 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/53948/1/NurFarhana.PANASIAN2016.pdf application/pdf en http://irep.iium.edu.my/53948/2/NurFarhana.PANASIAN2016_Poster.pdf Mat Nawi, Nur Farhana and Zainuddin, Zainul Ibrahim and Sha'ban, Munirah (2016) A preliminary study on the effects of radiation exposure on chondrocytes:: Sulphated Glycosaminoglycan (sGAG) content using cellular model. In: 3rd Pan-Asian Biomedical Science Conference 2016 Kuala Lumpur, 7 – 8 Dec 2016, Kuala Lumpur. (Unpublished)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic Q Science (General)
spellingShingle Q Science (General)
Mat Nawi, Nur Farhana
Zainuddin, Zainul Ibrahim
Sha'ban, Munirah
A preliminary study on the effects of radiation exposure on chondrocytes:: Sulphated Glycosaminoglycan (sGAG) content using cellular model
description Radiation is known to be harmful to human health. However, the effect of radiation at cellular level has yet to be established. This study evaluated the possible effects of radiation on monolayer cultured chondrocytes. Rabbit’s chondrocytes were isolated, serially cultured and divided into two groups; (1) non-irradiated and (2) irradiated. Group 2 cells were subjected to irradiation after it reaches 80-90% confluency. Both groups 1 and 2 cells were taken out from the incubator, placed into a temperature-controlled container and were transported to a typical X-ray examination room. After irradiating group 2, both cell groups were returned into the incubator. Both groups were evaluated using morphological evaluation and sulphated glycosaminoglycan (sGAG) production at passages 0, 1, 2, and 3. All groups exhibited comparable morphological appearances throughout the passages. Both cell groups experienced gradual changes from chondrocytic to fibroblastic morphology. The non-irradiated group showed a decreasing trend of sGAG concentration after each successive passage. It is suggestive that when the optimum proliferating conditions of these cells were manipulated, stress was induced in the cells. Meanwhile, the irradiated group showed an increasing trend in relative sGAG concentration after each passage. This particular phenomenon is unexpected in view of the known harmful nature associated with radiation. This study suggested that the introduction of radiation exposure could have certain effects on the cartilaginous extracellular matrix (ECM) production. It is postulated that the induced radiation created a yet to be explained situation, where the cells tend to recover, regenerate and secrete extracellular matrix. Basically, this finding warrants further evaluation where gene expression and histological analysis will be conducted. The limitation of study includes the number of samples used (n=2). Future work involving more samples and subjecting the cells to irradiation up to passage 5 will be conducted. Keywords: Glycosaminoglycan; cartilage; chondrocyte; radiation; X-ray; cellular model
format Conference or Workshop Item
author Mat Nawi, Nur Farhana
Zainuddin, Zainul Ibrahim
Sha'ban, Munirah
author_facet Mat Nawi, Nur Farhana
Zainuddin, Zainul Ibrahim
Sha'ban, Munirah
author_sort Mat Nawi, Nur Farhana
title A preliminary study on the effects of radiation exposure on chondrocytes:: Sulphated Glycosaminoglycan (sGAG) content using cellular model
title_short A preliminary study on the effects of radiation exposure on chondrocytes:: Sulphated Glycosaminoglycan (sGAG) content using cellular model
title_full A preliminary study on the effects of radiation exposure on chondrocytes:: Sulphated Glycosaminoglycan (sGAG) content using cellular model
title_fullStr A preliminary study on the effects of radiation exposure on chondrocytes:: Sulphated Glycosaminoglycan (sGAG) content using cellular model
title_full_unstemmed A preliminary study on the effects of radiation exposure on chondrocytes:: Sulphated Glycosaminoglycan (sGAG) content using cellular model
title_sort preliminary study on the effects of radiation exposure on chondrocytes:: sulphated glycosaminoglycan (sgag) content using cellular model
publishDate 2016
url http://irep.iium.edu.my/53948/1/NurFarhana.PANASIAN2016.pdf
http://irep.iium.edu.my/53948/2/NurFarhana.PANASIAN2016_Poster.pdf
http://irep.iium.edu.my/53948/
_version_ 1643614451741491200
score 13.211869

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