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Assessment of sulfide concentration effects in a microoxygenated condition on the growth and removal by pseudomonas putida (ATCC 49128)

Micro-oxygenation has recently being gaining popularity as a successful biological sulfide oxidation technique from inflicted wastewater. In this study the application of orbital shaker as an alternative digester for sulfide bioxidation was experimented using Pseudomonas putida (ATCC 49128), unde...

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Main Authors: Ahmad, Mani Malam, Azoddein, Abd.Aziz Mohd, Mohd Zahari, Mior Ahmad Khusairi, Abu Seman, Mazrul Nizam, Jami, Mohammed Saedi, Mohd Azli, Faten Ahada
Format: Article
Language:English
Published: The New Academia Press 2016
Subjects:
TP155 Chemical engineering
Online Access:http://irep.iium.edu.my/53780/1/Assessment%20of%20sulfide%20concentration%20effects%20in%20a%20microoxygenated%20%28Mani%2Cet%20al.%2C%202016%29.pdf
http://irep.iium.edu.my/53780/
http://www.akademiabaru.com/doc/ARMSV27_N1_P1_10.pdf
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spelling my.iium.irep.537802016-12-20T03:47:27Z http://irep.iium.edu.my/53780/ Assessment of sulfide concentration effects in a microoxygenated condition on the growth and removal by pseudomonas putida (ATCC 49128) Ahmad, Mani Malam Azoddein, Abd.Aziz Mohd Mohd Zahari, Mior Ahmad Khusairi Abu Seman, Mazrul Nizam Jami, Mohammed Saedi Mohd Azli, Faten Ahada TP155 Chemical engineering Micro-oxygenation has recently being gaining popularity as a successful biological sulfide oxidation technique from inflicted wastewater. In this study the application of orbital shaker as an alternative digester for sulfide bioxidation was experimented using Pseudomonas putida (ATCC 49128), under an oxygen tension or micro-oxygenated condition. Growth and sulfide reduction efficiency was measured spectrophotometrically under optimum physical conditions of pH, temperature, acclimatization time and agitation. Sulfide reduction was overwhelmingly recorded at three different sulfide loading rates of 200mM S2- L-1 d-1, 300mM S2- L-1 d-1 and 500mM S 2- L-1 d-1 with corresponding appreciable cell growth measured at OD600nm. The obtained results indicated that it was possible to realized sulfide removal efficiency of 96% to 100% within 24hrs, as well 45% to 70% within the first 6hrs of inoculation, with an overwhelming removal of 100% after 18hrs in 200mM and 300mM. On the other hand, the corresponding growth was 3.00 OD600nm, 2.824 OD600nm and 2.456 OD600nm in 500pmm, 200ppm and 300ppm concentration respectively. Based on this finding, it was clear that this inoculum can be employed to treat sulfide contaminated wastewater even at higher range under micro-oxygenated environmental condition. The New Academia Press 2016-12 Article REM application/pdf en http://irep.iium.edu.my/53780/1/Assessment%20of%20sulfide%20concentration%20effects%20in%20a%20microoxygenated%20%28Mani%2Cet%20al.%2C%202016%29.pdf Ahmad, Mani Malam and Azoddein, Abd.Aziz Mohd and Mohd Zahari, Mior Ahmad Khusairi and Abu Seman, Mazrul Nizam and Jami, Mohammed Saedi and Mohd Azli, Faten Ahada (2016) Assessment of sulfide concentration effects in a microoxygenated condition on the growth and removal by pseudomonas putida (ATCC 49128). Journal of Advanced Research in Materials Science, 27 (1). pp. 1-10. ISSN 2289-7992 http://www.akademiabaru.com/doc/ARMSV27_N1_P1_10.pdf
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP155 Chemical engineering
spellingShingle TP155 Chemical engineering
Ahmad, Mani Malam
Azoddein, Abd.Aziz Mohd
Mohd Zahari, Mior Ahmad Khusairi
Abu Seman, Mazrul Nizam
Jami, Mohammed Saedi
Mohd Azli, Faten Ahada
Assessment of sulfide concentration effects in a microoxygenated condition on the growth and removal by pseudomonas putida (ATCC 49128)
description Micro-oxygenation has recently being gaining popularity as a successful biological sulfide oxidation technique from inflicted wastewater. In this study the application of orbital shaker as an alternative digester for sulfide bioxidation was experimented using Pseudomonas putida (ATCC 49128), under an oxygen tension or micro-oxygenated condition. Growth and sulfide reduction efficiency was measured spectrophotometrically under optimum physical conditions of pH, temperature, acclimatization time and agitation. Sulfide reduction was overwhelmingly recorded at three different sulfide loading rates of 200mM S2- L-1 d-1, 300mM S2- L-1 d-1 and 500mM S 2- L-1 d-1 with corresponding appreciable cell growth measured at OD600nm. The obtained results indicated that it was possible to realized sulfide removal efficiency of 96% to 100% within 24hrs, as well 45% to 70% within the first 6hrs of inoculation, with an overwhelming removal of 100% after 18hrs in 200mM and 300mM. On the other hand, the corresponding growth was 3.00 OD600nm, 2.824 OD600nm and 2.456 OD600nm in 500pmm, 200ppm and 300ppm concentration respectively. Based on this finding, it was clear that this inoculum can be employed to treat sulfide contaminated wastewater even at higher range under micro-oxygenated environmental condition.
format Article
author Ahmad, Mani Malam
Azoddein, Abd.Aziz Mohd
Mohd Zahari, Mior Ahmad Khusairi
Abu Seman, Mazrul Nizam
Jami, Mohammed Saedi
Mohd Azli, Faten Ahada
author_facet Ahmad, Mani Malam
Azoddein, Abd.Aziz Mohd
Mohd Zahari, Mior Ahmad Khusairi
Abu Seman, Mazrul Nizam
Jami, Mohammed Saedi
Mohd Azli, Faten Ahada
author_sort Ahmad, Mani Malam
title Assessment of sulfide concentration effects in a microoxygenated condition on the growth and removal by pseudomonas putida (ATCC 49128)
title_short Assessment of sulfide concentration effects in a microoxygenated condition on the growth and removal by pseudomonas putida (ATCC 49128)
title_full Assessment of sulfide concentration effects in a microoxygenated condition on the growth and removal by pseudomonas putida (ATCC 49128)
title_fullStr Assessment of sulfide concentration effects in a microoxygenated condition on the growth and removal by pseudomonas putida (ATCC 49128)
title_full_unstemmed Assessment of sulfide concentration effects in a microoxygenated condition on the growth and removal by pseudomonas putida (ATCC 49128)
title_sort assessment of sulfide concentration effects in a microoxygenated condition on the growth and removal by pseudomonas putida (atcc 49128)
publisher The New Academia Press
publishDate 2016
url http://irep.iium.edu.my/53780/1/Assessment%20of%20sulfide%20concentration%20effects%20in%20a%20microoxygenated%20%28Mani%2Cet%20al.%2C%202016%29.pdf
http://irep.iium.edu.my/53780/
http://www.akademiabaru.com/doc/ARMSV27_N1_P1_10.pdf
_version_ 1643614415297183744
score 13.211869

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