Staff View: Highly sensitive Escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures

Highly sensitive Escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures

Surface acoustic wave mediated transductions have been widely used in the sensors and actuators applications. In this study, a shear horizontal surface acoustic wave (SHSAW) was used for the detection of food pathogenic Escherichia coli O157:H7 (E.coli O157:H7), a dangerous strain among 225 E. coli...

Full description

Saved in:

Bibliographic Details
Main Authors:	Ten, Seng Teik, Hashim, Uda, Gopinath, Subash C.B, Liu, W.W., Foo, K.L., Sam, S.T, Ab Rahman, Siti Fatimah, Voon, Chun Hong, Nordin, Anis Nurashikin
Format:	Article
Language:	English English English
Published:	Elsevier Limited 2017
Subjects:	TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices
Online Access:	http://irep.iium.edu.my/52752/7/52752_highly%20sensitive%20Eschherichia_text.pdf http://irep.iium.edu.my/52752/8/52752_highly%20sensitive%20Eschherichia_SCOPUS.pdf http://irep.iium.edu.my/52752/19/52752_highly%20sensitive%20Eschherichia_WOS.pdf http://irep.iium.edu.my/52752/ http://www.sciencedirect.com/science/article/pii/S0956566316309253
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.iium.irep.52752
record_format	dspace
spelling	my.iium.irep.527522018-05-16T08:42:41Z http://irep.iium.edu.my/52752/ Highly sensitive Escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures Ten, Seng Teik Hashim, Uda Gopinath, Subash C.B Liu, W.W. Foo, K.L. Sam, S.T Ab Rahman, Siti Fatimah Voon, Chun Hong Nordin, Anis Nurashikin TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices Surface acoustic wave mediated transductions have been widely used in the sensors and actuators applications. In this study, a shear horizontal surface acoustic wave (SHSAW) was used for the detection of food pathogenic Escherichia coli O157:H7 (E.coli O157:H7), a dangerous strain among 225 E. coli unique serotypes. A few cells of this bacterium are able to cause young children to be most vulnerable to serious complications. Presence of higher than 1 cfu E.coli O157:H7 in 25 g of food has been considered as a dangerous level. The SHSAW biosensor was fabricated on 64° YX LiNbO3 substrate. Its sensitivity was enhanced by depositing 130 nm thin layer of SiO2 nanostructures with particle size lesser than 70 nm. The nanostructures act both as a waveguide as well as a physical surface modification of the sensor prior to biomolecular immobilization. A specific DNA sequence from E. coli O157:H7 having 22 mers as an amine-terminated probe ssDNA was immobilized on the thin film sensing area through chemical functionalization [(CHO-(CH2)3-CHO) and APTES; NH2-(CH2)3-Si(OC2H5)3]. The high-performance of sensor was shown with the specific oligonucleotide tar- get and attained the sensitivity of 0.6439 nM/0.1 kHz and detection limit was down to 1.8 femto-molar (1.8×10−15 M). Further evidence was provided by specificity analysis using single mismatched and complementary oligonucleotide sequences. Elsevier Limited 2017-07-15 Article REM application/pdf en http://irep.iium.edu.my/52752/7/52752_highly%20sensitive%20Eschherichia_text.pdf application/pdf en http://irep.iium.edu.my/52752/8/52752_highly%20sensitive%20Eschherichia_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/52752/19/52752_highly%20sensitive%20Eschherichia_WOS.pdf Ten, Seng Teik and Hashim, Uda and Gopinath, Subash C.B and Liu, W.W. and Foo, K.L. and Sam, S.T and Ab Rahman, Siti Fatimah and Voon, Chun Hong and Nordin, Anis Nurashikin (2017) Highly sensitive Escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures. Biosensors and Bioelectronics, 93. pp. 146-154. ISSN 0956-5663 http://www.sciencedirect.com/science/article/pii/S0956566316309253 10.1016/j.bios.2016.09.035
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English English English
topic	TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices
spellingShingle	TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices Ten, Seng Teik Hashim, Uda Gopinath, Subash C.B Liu, W.W. Foo, K.L. Sam, S.T Ab Rahman, Siti Fatimah Voon, Chun Hong Nordin, Anis Nurashikin Highly sensitive Escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures
description	Surface acoustic wave mediated transductions have been widely used in the sensors and actuators applications. In this study, a shear horizontal surface acoustic wave (SHSAW) was used for the detection of food pathogenic Escherichia coli O157:H7 (E.coli O157:H7), a dangerous strain among 225 E. coli unique serotypes. A few cells of this bacterium are able to cause young children to be most vulnerable to serious complications. Presence of higher than 1 cfu E.coli O157:H7 in 25 g of food has been considered as a dangerous level. The SHSAW biosensor was fabricated on 64° YX LiNbO3 substrate. Its sensitivity was enhanced by depositing 130 nm thin layer of SiO2 nanostructures with particle size lesser than 70 nm. The nanostructures act both as a waveguide as well as a physical surface modification of the sensor prior to biomolecular immobilization. A specific DNA sequence from E. coli O157:H7 having 22 mers as an amine-terminated probe ssDNA was immobilized on the thin film sensing area through chemical functionalization [(CHO-(CH2)3-CHO) and APTES; NH2-(CH2)3-Si(OC2H5)3]. The high-performance of sensor was shown with the specific oligonucleotide tar- get and attained the sensitivity of 0.6439 nM/0.1 kHz and detection limit was down to 1.8 femto-molar (1.8×10−15 M). Further evidence was provided by specificity analysis using single mismatched and complementary oligonucleotide sequences.
format	Article
author	Ten, Seng Teik Hashim, Uda Gopinath, Subash C.B Liu, W.W. Foo, K.L. Sam, S.T Ab Rahman, Siti Fatimah Voon, Chun Hong Nordin, Anis Nurashikin
author_facet	Ten, Seng Teik Hashim, Uda Gopinath, Subash C.B Liu, W.W. Foo, K.L. Sam, S.T Ab Rahman, Siti Fatimah Voon, Chun Hong Nordin, Anis Nurashikin
author_sort	Ten, Seng Teik
title	Highly sensitive Escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures
title_short	Highly sensitive Escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures
title_full	Highly sensitive Escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures
title_fullStr	Highly sensitive Escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures
title_full_unstemmed	Highly sensitive Escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures
title_sort	highly sensitive escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures
publisher	Elsevier Limited
publishDate	2017
url	http://irep.iium.edu.my/52752/7/52752_highly%20sensitive%20Eschherichia_text.pdf http://irep.iium.edu.my/52752/8/52752_highly%20sensitive%20Eschherichia_SCOPUS.pdf http://irep.iium.edu.my/52752/19/52752_highly%20sensitive%20Eschherichia_WOS.pdf http://irep.iium.edu.my/52752/ http://www.sciencedirect.com/science/article/pii/S0956566316309253
_version_	1643614225045651456
score	13.211869

Highly sensitive Escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures

Similar Items