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Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet

Introduction: Collagen can be basically extracted by chemical and enzymatic hydrolysis. In many industries, chemical hydrolysis method is more commonly used. However, the enzymatic hydrolysis is more effective when the high nutritional value product will be produced based on the extracted material....

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Main Authors:	Mokhtar, Nur Dhiya, Abdul Wahab, Widya, Ab. Hadi, Hazrina, Abu Hassan, Muhamad Shafiq, Haron, Normah, Muhamad Bunnori, Noraslinda
Format:	Conference or Workshop Item
Language:	English
Published:	IIUM Medical Centre, Kuantan 2016
Subjects:	S Agriculture (General) SB Plant culture
Online Access:	http://irep.iium.edu.my/52589/1/medical%20research_%20complete.pdf http://irep.iium.edu.my/52589/
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spelling	my.iium.irep.525892017-07-13T07:26:05Z http://irep.iium.edu.my/52589/ Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet Mokhtar, Nur Dhiya Abdul Wahab, Widya Ab. Hadi, Hazrina Abu Hassan, Muhamad Shafiq Haron, Normah Muhamad Bunnori, Noraslinda S Agriculture (General) SB Plant culture Introduction: Collagen can be basically extracted by chemical and enzymatic hydrolysis. In many industries, chemical hydrolysis method is more commonly used. However, the enzymatic hydrolysis is more effective when the high nutritional value product will be produced based on the extracted material. Chicken (Gallus gallus domesticus) feet were used as a source of bird collagen. Method: Optimization of collagen extraction was investigated by using three different methods which were soaked in 5% lactic acid for 36 hours, 0.1% papain in 0.5 mol/L acetic acid and 0.1% bromelain in 0.5 mol/L acetic acid. Various properties such as percentage of end yield, concentration of protein, pH, swelling percentage and SDS-PAGE patterns of collagen from chicken feet were evaluated in order to determine the best method of extraction. Result: Type I collagen was considered as major component of chicken feet collagen which indicated by β chain and two distinct α-chains which are α1 and α2 that observed from electrophoresis pattern of collagen extracted from all three methods. Lactic acid method showed the highest percentage of end yield and concentration of protein compared to another two methods. However, lactic acid method showed the lowest swelling percentage and highest pH values. Conclusion: According to these results, soaked in 5% lactic acid for 36 hours is possible as the best collagen extraction method from chicken feet. IIUM Medical Centre, Kuantan 2016-08-04 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/52589/1/medical%20research_%20complete.pdf Mokhtar, Nur Dhiya and Abdul Wahab, Widya and Ab. Hadi, Hazrina and Abu Hassan, Muhamad Shafiq and Haron, Normah and Muhamad Bunnori, Noraslinda (2016) Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet. In: Medical Research Symposium (MRS) and Pacific Partnership in Conjunction with Kuantan Research Day 2016, 4 August 2016, Kulliyyah of Medicine, IIUM Kuantan. (Unpublished)
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English
topic	S Agriculture (General) SB Plant culture
spellingShingle	S Agriculture (General) SB Plant culture Mokhtar, Nur Dhiya Abdul Wahab, Widya Ab. Hadi, Hazrina Abu Hassan, Muhamad Shafiq Haron, Normah Muhamad Bunnori, Noraslinda Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet
description	Introduction: Collagen can be basically extracted by chemical and enzymatic hydrolysis. In many industries, chemical hydrolysis method is more commonly used. However, the enzymatic hydrolysis is more effective when the high nutritional value product will be produced based on the extracted material. Chicken (Gallus gallus domesticus) feet were used as a source of bird collagen. Method: Optimization of collagen extraction was investigated by using three different methods which were soaked in 5% lactic acid for 36 hours, 0.1% papain in 0.5 mol/L acetic acid and 0.1% bromelain in 0.5 mol/L acetic acid. Various properties such as percentage of end yield, concentration of protein, pH, swelling percentage and SDS-PAGE patterns of collagen from chicken feet were evaluated in order to determine the best method of extraction. Result: Type I collagen was considered as major component of chicken feet collagen which indicated by β chain and two distinct α-chains which are α1 and α2 that observed from electrophoresis pattern of collagen extracted from all three methods. Lactic acid method showed the highest percentage of end yield and concentration of protein compared to another two methods. However, lactic acid method showed the lowest swelling percentage and highest pH values. Conclusion: According to these results, soaked in 5% lactic acid for 36 hours is possible as the best collagen extraction method from chicken feet.
format	Conference or Workshop Item
author	Mokhtar, Nur Dhiya Abdul Wahab, Widya Ab. Hadi, Hazrina Abu Hassan, Muhamad Shafiq Haron, Normah Muhamad Bunnori, Noraslinda
author_facet	Mokhtar, Nur Dhiya Abdul Wahab, Widya Ab. Hadi, Hazrina Abu Hassan, Muhamad Shafiq Haron, Normah Muhamad Bunnori, Noraslinda
author_sort	Mokhtar, Nur Dhiya
title	Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet
title_short	Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet
title_full	Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet
title_fullStr	Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet
title_full_unstemmed	Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet
title_sort	extraction optimization and properties of collagen from chicken (gallus gallus domesticus) feet
publisher	IIUM Medical Centre, Kuantan
publishDate	2016
url	http://irep.iium.edu.my/52589/1/medical%20research_%20complete.pdf http://irep.iium.edu.my/52589/
_version_	1643614190352465920
score	13.211869

Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet

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