Cover Image

In vitro and in vivo wound healing studies of methanolic fraction of Centella Asiatica extract

Ethnopharmacological relevance: Asiaticoside is claimed as a bioactive compound capable of wound healing. In order to ensure that the pharmacological activity of the extract is traceable and measurable, the present study attempted to evaluate the bioactivity of rich fractionated extract of asiaticos...

Full description

Saved in:
Bibliographic Details
Main Authors: Abdul Aziz, Huwaida, Bakhtiar, M. Taher, Ahmed, Afnan Sh., Wan Sulaiman, Wan Mohd. Azizi, Darnis, Deny Susanti, Chowdhury, Shiplu Roy, Zakaria, Zainul Amiruddin
Format: Article
Language:English
English
Published: Elsevier 2017
Subjects:
RS Pharmacy and materia medica
Online Access:http://irep.iium.edu.my/52528/1/SAJB-1-s2.0-S0254629916302617-main.pdf
http://irep.iium.edu.my/52528/7/52528_In%20vitro%20and%20In%20vivo%20wound%20healing_scopus.pdf
http://irep.iium.edu.my/52528/
http://www.sciencedirect.com/science/article/pii/S0254629916302617
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.iium.irep.52528
record_format dspace
spelling my.iium.irep.525282016-12-04T17:40:43Z http://irep.iium.edu.my/52528/ In vitro and in vivo wound healing studies of methanolic fraction of Centella Asiatica extract Abdul Aziz, Huwaida Bakhtiar, M. Taher Ahmed, Afnan Sh. Wan Sulaiman, Wan Mohd. Azizi Darnis, Deny Susanti Chowdhury, Shiplu Roy Zakaria, Zainul Amiruddin RS Pharmacy and materia medica Ethnopharmacological relevance: Asiaticoside is claimed as a bioactive compound capable of wound healing. In order to ensure that the pharmacological activity of the extract is traceable and measurable, the present study attempted to evaluate the bioactivity of rich fractionated extract of asiaticoside. Aim of the study: The current study evaluates the wound healing efficacy via in vitro scratch assay and in vivo circular wound excision model. Materials and methods: The ethanol extract was fractionated into seven fractions via vacuum liquid chromatography. The compound of interest in the fractions was qualitatively identified using thin layer chromatography and the positive fraction containing asiaticoside was further quantified using reverse-phase HPLC. The asiaticoside-rich fraction was subjected to (i) colorimetric MTT (methylthiazoltetrazolium) cytotoxicity assay following incubation with human dermal fibroblast (HDF) and human dermal keratinocyte (HaCaT);(ii) in vitro 12-well plate scratch assay (using HDF and HaCaT cells) and (iii) topically apply (40%, 10% and 2.5%, w/w) on in vivo circular wound excision of rabbits. Data on wound contraction, epithelisation period, hydroxyproline content and histophatological analysis was collected from in vivo study. Results: The results showed that the methanol fraction of the extract contained about 2.4% asiaticoside. Based on the results of colorimetric MTT (methylthiazoltetrazolium) cytotoxicity assay, both HDF and HaCaT showed significant stimulation upon application of the methanolic fraction of extract at concentrations of 100 μg/mL and 0.19 μg/mL. The methanol fraction showed almost no toxicity effect at the concentrations tested since their IC50 could not be determined in concentrations ranging from 100 μg/mL to 0.19 μg/mL. Since all the concentrations tested allowed for more than 90% cell viability, the concentrations chosen for the scratch assay were randomly chosen and designated as highest (100 μg/mL), medium (6 μg/mL) and lowest (0.2 μg/mL) concentrations. In the scratch assay, methanol fraction of extract with concentration of 0.2 μg/mL and 100 μg/mL showed significant effect on HDF and HaCaT compared to the positive control (p b 0.05). In vivo, it was shown that the methanol fraction of the extract induced collagen synthesis. Histopathology data also concluded that dose-dependent effect of the tested extract as a wound healer was present. Conclusions: Taken together, recent findings suggest that methanol fraction of C. asiatica demonstrated remarkable polyvalent activity, and thus has potential as an effective wound healer. In conclusion, the claim of the presence of wound healing properties in C. asiatica had been well supported based on the results obtained in this study. Elsevier 2017 Article REM application/pdf en http://irep.iium.edu.my/52528/1/SAJB-1-s2.0-S0254629916302617-main.pdf application/pdf en http://irep.iium.edu.my/52528/7/52528_In%20vitro%20and%20In%20vivo%20wound%20healing_scopus.pdf Abdul Aziz, Huwaida and Bakhtiar, M. Taher and Ahmed, Afnan Sh. and Wan Sulaiman, Wan Mohd. Azizi and Darnis, Deny Susanti and Chowdhury, Shiplu Roy and Zakaria, Zainul Amiruddin (2017) In vitro and in vivo wound healing studies of methanolic fraction of Centella Asiatica extract. South African Journal of Botany, 108. pp. 163-174. ISSN 0254-6299 http://www.sciencedirect.com/science/article/pii/S0254629916302617 10.1016/j.sajb.2016.10.022
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic RS Pharmacy and materia medica
spellingShingle RS Pharmacy and materia medica
Abdul Aziz, Huwaida
Bakhtiar, M. Taher
Ahmed, Afnan Sh.
Wan Sulaiman, Wan Mohd. Azizi
Darnis, Deny Susanti
Chowdhury, Shiplu Roy
Zakaria, Zainul Amiruddin
In vitro and in vivo wound healing studies of methanolic fraction of Centella Asiatica extract
description Ethnopharmacological relevance: Asiaticoside is claimed as a bioactive compound capable of wound healing. In order to ensure that the pharmacological activity of the extract is traceable and measurable, the present study attempted to evaluate the bioactivity of rich fractionated extract of asiaticoside. Aim of the study: The current study evaluates the wound healing efficacy via in vitro scratch assay and in vivo circular wound excision model. Materials and methods: The ethanol extract was fractionated into seven fractions via vacuum liquid chromatography. The compound of interest in the fractions was qualitatively identified using thin layer chromatography and the positive fraction containing asiaticoside was further quantified using reverse-phase HPLC. The asiaticoside-rich fraction was subjected to (i) colorimetric MTT (methylthiazoltetrazolium) cytotoxicity assay following incubation with human dermal fibroblast (HDF) and human dermal keratinocyte (HaCaT);(ii) in vitro 12-well plate scratch assay (using HDF and HaCaT cells) and (iii) topically apply (40%, 10% and 2.5%, w/w) on in vivo circular wound excision of rabbits. Data on wound contraction, epithelisation period, hydroxyproline content and histophatological analysis was collected from in vivo study. Results: The results showed that the methanol fraction of the extract contained about 2.4% asiaticoside. Based on the results of colorimetric MTT (methylthiazoltetrazolium) cytotoxicity assay, both HDF and HaCaT showed significant stimulation upon application of the methanolic fraction of extract at concentrations of 100 μg/mL and 0.19 μg/mL. The methanol fraction showed almost no toxicity effect at the concentrations tested since their IC50 could not be determined in concentrations ranging from 100 μg/mL to 0.19 μg/mL. Since all the concentrations tested allowed for more than 90% cell viability, the concentrations chosen for the scratch assay were randomly chosen and designated as highest (100 μg/mL), medium (6 μg/mL) and lowest (0.2 μg/mL) concentrations. In the scratch assay, methanol fraction of extract with concentration of 0.2 μg/mL and 100 μg/mL showed significant effect on HDF and HaCaT compared to the positive control (p b 0.05). In vivo, it was shown that the methanol fraction of the extract induced collagen synthesis. Histopathology data also concluded that dose-dependent effect of the tested extract as a wound healer was present. Conclusions: Taken together, recent findings suggest that methanol fraction of C. asiatica demonstrated remarkable polyvalent activity, and thus has potential as an effective wound healer. In conclusion, the claim of the presence of wound healing properties in C. asiatica had been well supported based on the results obtained in this study.
format Article
author Abdul Aziz, Huwaida
Bakhtiar, M. Taher
Ahmed, Afnan Sh.
Wan Sulaiman, Wan Mohd. Azizi
Darnis, Deny Susanti
Chowdhury, Shiplu Roy
Zakaria, Zainul Amiruddin
author_facet Abdul Aziz, Huwaida
Bakhtiar, M. Taher
Ahmed, Afnan Sh.
Wan Sulaiman, Wan Mohd. Azizi
Darnis, Deny Susanti
Chowdhury, Shiplu Roy
Zakaria, Zainul Amiruddin
author_sort Abdul Aziz, Huwaida
title In vitro and in vivo wound healing studies of methanolic fraction of Centella Asiatica extract
title_short In vitro and in vivo wound healing studies of methanolic fraction of Centella Asiatica extract
title_full In vitro and in vivo wound healing studies of methanolic fraction of Centella Asiatica extract
title_fullStr In vitro and in vivo wound healing studies of methanolic fraction of Centella Asiatica extract
title_full_unstemmed In vitro and in vivo wound healing studies of methanolic fraction of Centella Asiatica extract
title_sort in vitro and in vivo wound healing studies of methanolic fraction of centella asiatica extract
publisher Elsevier
publishDate 2017
url http://irep.iium.edu.my/52528/1/SAJB-1-s2.0-S0254629916302617-main.pdf
http://irep.iium.edu.my/52528/7/52528_In%20vitro%20and%20In%20vivo%20wound%20healing_scopus.pdf
http://irep.iium.edu.my/52528/
http://www.sciencedirect.com/science/article/pii/S0254629916302617
_version_ 1643614182218661888
score 13.211869

Similar Items