Simulation of absorption spectrum of photosynthetic pigments of chlorella vulgaris B algae
Absorption spectrum of Chlorella vulgaris B algae was reconstructed by simulated the absorption spectrum of the mixture under examination by a linear combination of the absorption spectra of all its constituents. A C30 reversed high performance liquid chromatography equipped with photodiode arra...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Institute for Mathematical Research (INSPEM), Universiti Putra Malaysia
2016
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/50717/6/50717-article.pdf http://irep.iium.edu.my/50717/7/50717-Simulation%20of%20absorption%20spectrum%20of%20photosynthetic%20pigments%20of%20chlorella%20vulgaris%20B%20algae_SCOPUS.pdf http://irep.iium.edu.my/50717/ http://einspem.upm.edu.my/journal/fullpaper/vol10sfeb/No11.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Absorption spectrum of Chlorella vulgaris B algae was reconstructed by
simulated the absorption spectrum of the mixture under examination by a
linear combination of the absorption spectra of all its constituents. A C30
reversed high performance liquid chromatography equipped with photodiode
array detector (HPLC-PAD) and absorption spectrophotometer
were used to separate and identify photosynthetic pigments of Chlorella
vulgaris B. Spectrum-reconstruction method (SRCM) was used to calculate
concentrations of total carotenoids, chlorophylls a and b. Absorption
spectrum of the total pigments extracted of Chlorella vulgaris B.
was reconstructed from the sum of the spectrum of individual extracted
pigments taken from (HPLC-PAD), and compared using normalization
with absorption spectrum of the total pigments recorded by spectrophotometer.
A total of seven Pigments were separated and identified |
|---|