Production of bioethanol by direct bioconversion of oil-palm industrial effluent in a stirred-tank bioreactor
The purpose of this study was to evaluate the feasibility of producing bioethanol from palm-oil mill effluent generated by the oil-palm industries through direct bioconversion process. The bioethanol production was carried out through the treatment of compatible mixed cultures such as Thrichode...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer Science
2009
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/5062/1/Paper-bioethanol-JIMB.pdf http://irep.iium.edu.my/5062/ http://dx.doi.org/10.1007/s10295-009-0554-7 |
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| Summary: | The purpose of this study was to evaluate the
feasibility of producing bioethanol from palm-oil mill
effluent generated by the oil-palm industries through direct
bioconversion process. The bioethanol production was
carried out through the treatment of compatible mixed
cultures such as Thrichoderma harzianum, Phanerochaete
chrysosporium, Mucor hiemalis, and yeast, Saccharomyces
cerevisiae. Simultaneous inoculation of T. harzianum and
S. cerevisiae was found to be the mixed culture that yielded
the highest ethanol production (4% v/v or 31.6 g/l).
Statistical optimization was carried out to determine the
operating conditions of the stirred-tank bioreactor for
maximum bioethanol production by a two-level fractional
factorial design with a single central point. The factors
involved were oxygen saturation level (pO2%), temperature,
and pH. A polynomial regression model was developed using the experimental data including the linear,quadratic, and interaction effects. Statistical analysis showed that the maximum ethanol production of 4.6% (v/v) or 36.3 g/l was achieved at a temperature of 32�C, pH of 6, and pO2 of 30%. The results of the model validation test under the developed optimum process conditions indicated that the maximum production was increased from 4.6% (v/v) to 6.5% (v/v) or 51.3 g/l with 89.1% chemicaloxygen-demand removal. |
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