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Bromelain enzyme assay using Casen as substrate

This procedure is following Sigma's non-specific protease activity. In this assay, casein acts as a substrate to the reaction. When protease digest casein, the amino acid tyrosine is liberated along with other amino acids and peptide fragments. Folin & Ciocalteus Phenol, or Folin's rea...

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Main Authors: Amid, Azura, Jamaluddin, Mohd Jamil Aizat
Format: Book Chapter
Language:English
Published: IIUM Press 2016
Subjects:
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/49849/1/49849_Bromelain_enzyme_assay_using_casein.pdf
http://irep.iium.edu.my/49849/
http://iiumpress.iium.edu.my/bookshop/experimental-methods-in-modern-biotechnology
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spelling my.iium.irep.498492017-02-21T02:05:49Z http://irep.iium.edu.my/49849/ Bromelain enzyme assay using Casen as substrate Amid, Azura Jamaluddin, Mohd Jamil Aizat TP248.13 Biotechnology This procedure is following Sigma's non-specific protease activity. In this assay, casein acts as a substrate to the reaction. When protease digest casein, the amino acid tyrosine is liberated along with other amino acids and peptide fragments. Folin & Ciocalteus Phenol, or Folin's reagent primarily reacts with free tyrosine to produce a blue colored chromophore, which is quantifiable and measured as an absorbance value on the spectrophotometer at 660 nm absorbance. The more tyrosine that is released from the casein, the more chromophores are generated and the stronger the activity of the protease. IIUM Press 2016 Book Chapter REM application/pdf en http://irep.iium.edu.my/49849/1/49849_Bromelain_enzyme_assay_using_casein.pdf Amid, Azura and Jamaluddin, Mohd Jamil Aizat (2016) Bromelain enzyme assay using Casen as substrate. In: Experimental methods in modern biotechnology Volume 2. IIUM Press Series of Textbook from the Islamic Perspectives, 2 . IIUM Press, Kuala Lumpur, pp. 100-150. ISBN 978-967-418-384-4 http://iiumpress.iium.edu.my/bookshop/experimental-methods-in-modern-biotechnology
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Amid, Azura
Jamaluddin, Mohd Jamil Aizat
Bromelain enzyme assay using Casen as substrate
description This procedure is following Sigma's non-specific protease activity. In this assay, casein acts as a substrate to the reaction. When protease digest casein, the amino acid tyrosine is liberated along with other amino acids and peptide fragments. Folin & Ciocalteus Phenol, or Folin's reagent primarily reacts with free tyrosine to produce a blue colored chromophore, which is quantifiable and measured as an absorbance value on the spectrophotometer at 660 nm absorbance. The more tyrosine that is released from the casein, the more chromophores are generated and the stronger the activity of the protease.
format Book Chapter
author Amid, Azura
Jamaluddin, Mohd Jamil Aizat
author_facet Amid, Azura
Jamaluddin, Mohd Jamil Aizat
author_sort Amid, Azura
title Bromelain enzyme assay using Casen as substrate
title_short Bromelain enzyme assay using Casen as substrate
title_full Bromelain enzyme assay using Casen as substrate
title_fullStr Bromelain enzyme assay using Casen as substrate
title_full_unstemmed Bromelain enzyme assay using Casen as substrate
title_sort bromelain enzyme assay using casen as substrate
publisher IIUM Press
publishDate 2016
url http://irep.iium.edu.my/49849/1/49849_Bromelain_enzyme_assay_using_casein.pdf
http://irep.iium.edu.my/49849/
http://iiumpress.iium.edu.my/bookshop/experimental-methods-in-modern-biotechnology
_version_ 1643613609954115584
score 13.211869

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