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Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase

This procedure is following Gulati, Saxena, and Gupta (1997) protocol which is known as semi-quantitative plate assay for screening L-asparaginase- producing bacterial from local hot spring. The plate agar medium containing asparagine (as sole nitrogen sourse) is prepared with addition of phenol red...

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Main Authors:	Jimat, Dzun Noraini, Mohamed , Intan Baizura Firda
Other Authors:	Jamal, Parveen
Format:	Book Chapter
Language:	English
Published:	IIUM Press 2016
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/49762/1/49762_Isolation_and_characterisation_of_thermophilic_bacterial_isolates_producing_L-asparaginase.pdf http://irep.iium.edu.my/49762/ http://iiumpress.iium.edu.my/bookshop/experimental-methods-in-modern-biotechnology
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spelling	my.iium.irep.497622017-01-16T06:23:28Z http://irep.iium.edu.my/49762/ Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase Jimat, Dzun Noraini Mohamed , Intan Baizura Firda TP248.13 Biotechnology This procedure is following Gulati, Saxena, and Gupta (1997) protocol which is known as semi-quantitative plate assay for screening L-asparaginase- producing bacterial from local hot spring. The plate agar medium containing asparagine (as sole nitrogen sourse) is prepared with addition of phenol red which later shows an indication of L-asparaginase based on changes of colour. Phenol red at acidic is yellow which might change colour to pink when it is at alkaline. Thus, it indicates the formation of pink zone around microbial colonies producing L-asparaginase. The inoculated agar plates are incubated at 370C in an incubator for 24 hours. Thus, the isolation and screening of bacterial producing L-asparaginase are based on phenol-red zone. After purification, the isolates are characterised using several biochemical tests. IIUM Press Jamal, Parveen Azmi, Azlin Suhaida Ibrahim Ali , Noorbatcha 2016-01 Book Chapter REM application/pdf en http://irep.iium.edu.my/49762/1/49762_Isolation_and_characterisation_of_thermophilic_bacterial_isolates_producing_L-asparaginase.pdf Jimat, Dzun Noraini and Mohamed , Intan Baizura Firda (2016) Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase. In: Experimental Methods in Modern Biotechnology. IIUM Press Series of Textbook from the Islamic Perspectives, 2 . IIUM Press, Kuala Lumpur, pp. 72-80. ISBN 978-967-418-384-4 http://iiumpress.iium.edu.my/bookshop/experimental-methods-in-modern-biotechnology
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Jimat, Dzun Noraini Mohamed , Intan Baizura Firda Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase
description	This procedure is following Gulati, Saxena, and Gupta (1997) protocol which is known as semi-quantitative plate assay for screening L-asparaginase- producing bacterial from local hot spring. The plate agar medium containing asparagine (as sole nitrogen sourse) is prepared with addition of phenol red which later shows an indication of L-asparaginase based on changes of colour. Phenol red at acidic is yellow which might change colour to pink when it is at alkaline. Thus, it indicates the formation of pink zone around microbial colonies producing L-asparaginase. The inoculated agar plates are incubated at 370C in an incubator for 24 hours. Thus, the isolation and screening of bacterial producing L-asparaginase are based on phenol-red zone. After purification, the isolates are characterised using several biochemical tests.
author2	Jamal, Parveen
author_facet	Jamal, Parveen Jimat, Dzun Noraini Mohamed , Intan Baizura Firda
format	Book Chapter
author	Jimat, Dzun Noraini Mohamed , Intan Baizura Firda
author_sort	Jimat, Dzun Noraini
title	Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase
title_short	Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase
title_full	Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase
title_fullStr	Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase
title_full_unstemmed	Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase
title_sort	isolation and characterisation of thermophilic bacterial isolates producing l-asparaginase
publisher	IIUM Press
publishDate	2016
url	http://irep.iium.edu.my/49762/1/49762_Isolation_and_characterisation_of_thermophilic_bacterial_isolates_producing_L-asparaginase.pdf http://irep.iium.edu.my/49762/ http://iiumpress.iium.edu.my/bookshop/experimental-methods-in-modern-biotechnology
_version_	1643613594618691584
score	13.211869

Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase

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