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Proliferative and genotoxic effect of Perivitelline fluid from horseshoe crab on human dental pulp stem cells

The aim of this study was to evaluate the effect of perivitelline fluid (PVF) on the proliferation, chromosome aberration and mutagenicity of dental pulp stem cells (DPSCs). Crude extracts were prepared from the PVF collected from horseshoe crabs. For cytotoxicity test (MTT assay), the DPSCs were...

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Main Authors: Thirumulu Ponnuraj, Kannan, Musa, Marahaini, Mohd Ali, Khadijah, Ahmad , Azlina, Omar, Nor Shamsuria, Chatterji, Anil, Mokhtar@Makhtar, Khairani Idah
Format: Conference or Workshop Item
Language:English
English
Published: 2015
Subjects:
RK Dentistry
Online Access:http://irep.iium.edu.my/47662/1/KIM-ISSCR_abstract_2015.pdf
http://irep.iium.edu.my/47662/2/ISSCR_poster_Kannan_2015_%281%29_%281%29.pdf
http://irep.iium.edu.my/47662/
http://www.isscr.org/home/annual-meeting/past-future-meetings/isscr2015
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spelling my.iium.irep.476622016-01-27T02:33:31Z http://irep.iium.edu.my/47662/ Proliferative and genotoxic effect of Perivitelline fluid from horseshoe crab on human dental pulp stem cells Thirumulu Ponnuraj, Kannan Musa, Marahaini Mohd Ali, Khadijah Ahmad , Azlina Omar, Nor Shamsuria Chatterji, Anil Mokhtar@Makhtar, Khairani Idah RK Dentistry The aim of this study was to evaluate the effect of perivitelline fluid (PVF) on the proliferation, chromosome aberration and mutagenicity of dental pulp stem cells (DPSCs). Crude extracts were prepared from the PVF collected from horseshoe crabs. For cytotoxicity test (MTT assay), the DPSCs were subjected to different concentrations of PVF crude extract. Two inhibitory concentrations (IC50=26.887mg/ ml and IC25=14.093mg/ml) and two other concentrations (0.278mg/ml and 0.019mg/ ml) which produced higher cell viability were selected for downstream experiments. The proliferative effect was assessed using AlamarBlue® assay for a period of 10 days and the population doubling time (PDT) were also calculated based on this assay. Chromosome aberration and Ames tests were employed for the evaluating the genotoxicity. Statistical analysis was carried out using independent t-test to assess the significant differences (p<0.05). AlamarBlue® assay showed that PVF groups produced comparable proliferation activity to negative (untreated) control. The PDTs between PVF groups and negative control were also insignificantly different (p>0.05). Both the chromosome aberration test and Ames test did not show any significant differences in the PVF treated groups. Hence, it can be concluded that PVF from horseshoe crabs produced insignificant proliferative activity and was non-genotoxic on DPSCs based on chromosome aberration and Ames tests. 2015-06-24 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/47662/1/KIM-ISSCR_abstract_2015.pdf application/pdf en http://irep.iium.edu.my/47662/2/ISSCR_poster_Kannan_2015_%281%29_%281%29.pdf Thirumulu Ponnuraj, Kannan and Musa, Marahaini and Mohd Ali, Khadijah and Ahmad , Azlina and Omar, Nor Shamsuria and Chatterji, Anil and Mokhtar@Makhtar, Khairani Idah (2015) Proliferative and genotoxic effect of Perivitelline fluid from horseshoe crab on human dental pulp stem cells. In: ISSCR 2015 Annual Meeting, 24th-27th June 2015, Stockholm, Sweden. (Unpublished) http://www.isscr.org/home/annual-meeting/past-future-meetings/isscr2015
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic RK Dentistry
spellingShingle RK Dentistry
Thirumulu Ponnuraj, Kannan
Musa, Marahaini
Mohd Ali, Khadijah
Ahmad , Azlina
Omar, Nor Shamsuria
Chatterji, Anil
Mokhtar@Makhtar, Khairani Idah
Proliferative and genotoxic effect of Perivitelline fluid from horseshoe crab on human dental pulp stem cells
description The aim of this study was to evaluate the effect of perivitelline fluid (PVF) on the proliferation, chromosome aberration and mutagenicity of dental pulp stem cells (DPSCs). Crude extracts were prepared from the PVF collected from horseshoe crabs. For cytotoxicity test (MTT assay), the DPSCs were subjected to different concentrations of PVF crude extract. Two inhibitory concentrations (IC50=26.887mg/ ml and IC25=14.093mg/ml) and two other concentrations (0.278mg/ml and 0.019mg/ ml) which produced higher cell viability were selected for downstream experiments. The proliferative effect was assessed using AlamarBlue® assay for a period of 10 days and the population doubling time (PDT) were also calculated based on this assay. Chromosome aberration and Ames tests were employed for the evaluating the genotoxicity. Statistical analysis was carried out using independent t-test to assess the significant differences (p<0.05). AlamarBlue® assay showed that PVF groups produced comparable proliferation activity to negative (untreated) control. The PDTs between PVF groups and negative control were also insignificantly different (p>0.05). Both the chromosome aberration test and Ames test did not show any significant differences in the PVF treated groups. Hence, it can be concluded that PVF from horseshoe crabs produced insignificant proliferative activity and was non-genotoxic on DPSCs based on chromosome aberration and Ames tests.
format Conference or Workshop Item
author Thirumulu Ponnuraj, Kannan
Musa, Marahaini
Mohd Ali, Khadijah
Ahmad , Azlina
Omar, Nor Shamsuria
Chatterji, Anil
Mokhtar@Makhtar, Khairani Idah
author_facet Thirumulu Ponnuraj, Kannan
Musa, Marahaini
Mohd Ali, Khadijah
Ahmad , Azlina
Omar, Nor Shamsuria
Chatterji, Anil
Mokhtar@Makhtar, Khairani Idah
author_sort Thirumulu Ponnuraj, Kannan
title Proliferative and genotoxic effect of Perivitelline fluid from horseshoe crab on human dental pulp stem cells
title_short Proliferative and genotoxic effect of Perivitelline fluid from horseshoe crab on human dental pulp stem cells
title_full Proliferative and genotoxic effect of Perivitelline fluid from horseshoe crab on human dental pulp stem cells
title_fullStr Proliferative and genotoxic effect of Perivitelline fluid from horseshoe crab on human dental pulp stem cells
title_full_unstemmed Proliferative and genotoxic effect of Perivitelline fluid from horseshoe crab on human dental pulp stem cells
title_sort proliferative and genotoxic effect of perivitelline fluid from horseshoe crab on human dental pulp stem cells
publishDate 2015
url http://irep.iium.edu.my/47662/1/KIM-ISSCR_abstract_2015.pdf
http://irep.iium.edu.my/47662/2/ISSCR_poster_Kannan_2015_%281%29_%281%29.pdf
http://irep.iium.edu.my/47662/
http://www.isscr.org/home/annual-meeting/past-future-meetings/isscr2015
_version_ 1643613235697418240
score 13.211869

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