Ion-selective electrode biochip for applications in a liquid environment
Physiological sensing conducted in a liquid environment requires electrodes with long lifetime. The development of a robust ion-selective electrode–based biochip in a lab-on-a-chip platform is described. To compare electrode lifetime, which is driven by the transducer layer, electrochemical measurem...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Conference or Workshop Item |
| Language: | English English |
| Published: |
Springer
2016
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/47221/7/47221_Ion-selective%20electrode%20biochip%20for%20applications_SCOPUS.pdf http://irep.iium.edu.my/47221/13/47221_Ion-selective%20electrode%20biochip%20for%20applications%20in%20a%20liquid%20environment.pdf http://irep.iium.edu.my/47221/ http://www.springer.com/series/7403 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Physiological sensing conducted in a liquid environment requires electrodes with long lifetime. The development of a robust ion-selective electrode–based biochip in a lab-on-a-chip platform is described. To compare electrode lifetime, which is driven by the transducer layer, electrochemical measurements were performed in a custom-made flow-cell chamber. The results of potentiometric measurement of cationic analytes demonstrate the electrodes to have a near-Nernstian slope profile even after they are stored for almost a month in liquid medium. The electrodes also achieved H2O2 amperometric sensitivity (1.25 and 3.32 µAmM-1cm-2 for PEDOT:PSS and PEDOT:CaSO4 respectively) and lower detection limit (2.21 µM, 8.4 µM, 3.44 µM, for H+, NH4+, Ca2+ respectively) comparable to that of wire-type electrodes. Furthermore, the lifetime is dependent on the electrodeposition method of the conductive polymer, and the transducer layer must be modified to fit the analyte types. These results indicate that extended lifetime of microfabricated ion-selective electrodes in a multiplex format can be realized by optimizing the microfabricated electrode surface functionalization. |
|---|